GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31

Margaret Caroline MacHin Smith, C J INGHAM, C E OWEN, N T WOOD

Research output: Contribution to journalArticle

Abstract

The repressor gene, c, of the temperate Streptomyces phage, phi-C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein, The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi-C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi-C31 terminators display sequence conservation and may be regulated. The phi-C31 gene 'k' may encode a nucleotide kinase-encoding gene.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalGene
Volume115
Issue number1-2
Publication statusPublished - 15 Jun 1992

Keywords

  • REPRESSOR
  • TRANSCRIPTION
  • PROMOTER
  • TERMINATOR
  • REGULATION
  • OPERATOR
  • TRANSCRIPTIONAL ANALYSIS
  • NUCLEOTIDE-SEQUENCE
  • PHAGE PHI-C31
  • PROMOTERS

Cite this

Smith, M. C. M., INGHAM, C. J., OWEN, C. E., & WOOD, N. T. (1992). GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31. Gene, 115(1-2), 43-48.

GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31. / Smith, Margaret Caroline MacHin; INGHAM, C J ; OWEN, C E ; WOOD, N T .

In: Gene, Vol. 115, No. 1-2, 15.06.1992, p. 43-48.

Research output: Contribution to journalArticle

Smith, MCM, INGHAM, CJ, OWEN, CE & WOOD, NT 1992, 'GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31', Gene, vol. 115, no. 1-2, pp. 43-48.
Smith MCM, INGHAM CJ, OWEN CE, WOOD NT. GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31. Gene. 1992 Jun 15;115(1-2):43-48.
Smith, Margaret Caroline MacHin ; INGHAM, C J ; OWEN, C E ; WOOD, N T . / GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31. In: Gene. 1992 ; Vol. 115, No. 1-2. pp. 43-48.
@article{e1e2661786514784a8b23ba41ee561dc,
title = "GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31",
abstract = "The repressor gene, c, of the temperate Streptomyces phage, phi-C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein, The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi-C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi-C31 terminators display sequence conservation and may be regulated. The phi-C31 gene 'k' may encode a nucleotide kinase-encoding gene.",
keywords = "REPRESSOR, TRANSCRIPTION, PROMOTER, TERMINATOR, REGULATION, OPERATOR, TRANSCRIPTIONAL ANALYSIS, NUCLEOTIDE-SEQUENCE, PHAGE PHI-C31, PROMOTERS",
author = "Smith, {Margaret Caroline MacHin} and INGHAM, {C J} and OWEN, {C E} and WOOD, {N T}",
year = "1992",
month = "6",
day = "15",
language = "English",
volume = "115",
pages = "43--48",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31

AU - Smith, Margaret Caroline MacHin

AU - INGHAM, C J

AU - OWEN, C E

AU - WOOD, N T

PY - 1992/6/15

Y1 - 1992/6/15

N2 - The repressor gene, c, of the temperate Streptomyces phage, phi-C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein, The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi-C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi-C31 terminators display sequence conservation and may be regulated. The phi-C31 gene 'k' may encode a nucleotide kinase-encoding gene.

AB - The repressor gene, c, of the temperate Streptomyces phage, phi-C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein, The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi-C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi-C31 terminators display sequence conservation and may be regulated. The phi-C31 gene 'k' may encode a nucleotide kinase-encoding gene.

KW - REPRESSOR

KW - TRANSCRIPTION

KW - PROMOTER

KW - TERMINATOR

KW - REGULATION

KW - OPERATOR

KW - TRANSCRIPTIONAL ANALYSIS

KW - NUCLEOTIDE-SEQUENCE

KW - PHAGE PHI-C31

KW - PROMOTERS

M3 - Article

VL - 115

SP - 43

EP - 48

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -