GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31

Margaret Caroline MacHin Smith, C J INGHAM, C E OWEN, N T WOOD

Research output: Contribution to journalArticle

Abstract

The repressor gene, c, of the temperate Streptomyces phage, phi-C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein, The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi-C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi-C31 terminators display sequence conservation and may be regulated. The phi-C31 gene 'k' may encode a nucleotide kinase-encoding gene.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalGene
Volume115
Issue number1-2
Publication statusPublished - 15 Jun 1992

Keywords

  • REPRESSOR
  • TRANSCRIPTION
  • PROMOTER
  • TERMINATOR
  • REGULATION
  • OPERATOR
  • TRANSCRIPTIONAL ANALYSIS
  • NUCLEOTIDE-SEQUENCE
  • PHAGE PHI-C31
  • PROMOTERS

Cite this

Smith, M. C. M., INGHAM, C. J., OWEN, C. E., & WOOD, N. T. (1992). GENE-EXPRESSION IN THE STREPTOMYCES TEMPERATE PHAGE-PHI-C31. Gene, 115(1-2), 43-48.