Abstract
The repressor gene, c, of the temperate Streptomyces phage, phi-C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein, The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi-C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi-C31 terminators display sequence conservation and may be regulated. The phi-C31 gene 'k' may encode a nucleotide kinase-encoding gene.
Original language | English |
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Pages (from-to) | 43-48 |
Number of pages | 6 |
Journal | Gene |
Volume | 115 |
Issue number | 1-2 |
Publication status | Published - 15 Jun 1992 |
Keywords
- REPRESSOR
- TRANSCRIPTION
- PROMOTER
- TERMINATOR
- REGULATION
- OPERATOR
- TRANSCRIPTIONAL ANALYSIS
- NUCLEOTIDE-SEQUENCE
- PHAGE PHI-C31
- PROMOTERS