Gene expression profiling in naïve and vaccinated rainbow trout after Yersinia ruckeri infection

insights into the mechanisms of protection seen in vaccinated fish

Nor Omaima Harun, Tiehui Wang, Chris J Secombes

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Despite the importance and success of vaccination against bacterial diseases in fish, little is known about the mechanisms of vaccine-induced disease resistance. In this study a known efficacious bacterial vaccine, to Enteric Redmouth Disease (ERM), was used to vaccinate rainbow trout, and sixty days later the fish were challenged with the causative agent of the disease, Yersinia ruckeri. The bacterial burden in the spleen, the spleen index, and the expression profiles of pro- and anti-inflammatory cytokines and marker genes for T helper (Th) cells in the spleen and gills were analyzed, in comparison to the profiles in naïve/challenged fish. As expected, the bacterial burden in the spleen of naïve fish increased over time and was correlated with the spleen index after Y. ruckeri challenge. The gene expression data showed that pro-inflammatory cytokines were upregulated post-infection in the spleen of both naïve and vaccinated fish after Y. ruckeri challenge although the pro-inflammatory cytokine expression was much lower in vaccinated fish compared to the naïve fish. A correlated expression between pro-inflammatory cytokines and anti-inflammatory cytokines was only seen in spleen of ERM vaccinated fish, where a Th1-like response was indicated by the correlated gene expression of IFN-γ, T-bet and IL-2. In contrast, in the gills, the inflammatory gene response was enhanced in vaccinated fish compared to naïve fish, but perhaps more importantly there was a strong upregulation of IL-22 which was negatively correlated with IFN-γ gene expression at this site. Thus, it is possible that different types of adaptive responses are on-going within the vaccinated fish during infection with Y. ruckeri, potentially affected by the site and stage of infection.
Original languageEnglish
Pages (from-to)4388-4399
Number of pages12
JournalVaccine
Volume29
Issue number26
DOIs
Publication statusPublished - 10 Jun 2011

Fingerprint

Yersinia ruckeri
Yersinia Infections
Oncorhynchus mykiss
Gene Expression Profiling
Fishes
gene expression
Spleen
spleen
fish
infection
cytokines
Cytokines
Fish Diseases
Gene Expression
Anti-Inflammatory Agents
Infection
gills
Bacterial Vaccines
Disease Resistance
fish diseases

Keywords

  • animals
  • bacterial vaccines
  • cytokines
  • fish diseases
  • gene expression profiling
  • gene expression regulation
  • gills
  • Oncorhynchus mykiss
  • spleen
  • vaccination
  • yersinia infections
  • Yersinia ruckeri

Cite this

Gene expression profiling in naïve and vaccinated rainbow trout after Yersinia ruckeri infection : insights into the mechanisms of protection seen in vaccinated fish. / Harun, Nor Omaima; Wang, Tiehui; Secombes, Chris J.

In: Vaccine, Vol. 29, No. 26, 10.06.2011, p. 4388-4399.

Research output: Contribution to journalArticle

@article{b0c90a5ece474767b2718f02346cfde1,
title = "Gene expression profiling in na{\"i}ve and vaccinated rainbow trout after Yersinia ruckeri infection: insights into the mechanisms of protection seen in vaccinated fish",
abstract = "Despite the importance and success of vaccination against bacterial diseases in fish, little is known about the mechanisms of vaccine-induced disease resistance. In this study a known efficacious bacterial vaccine, to Enteric Redmouth Disease (ERM), was used to vaccinate rainbow trout, and sixty days later the fish were challenged with the causative agent of the disease, Yersinia ruckeri. The bacterial burden in the spleen, the spleen index, and the expression profiles of pro- and anti-inflammatory cytokines and marker genes for T helper (Th) cells in the spleen and gills were analyzed, in comparison to the profiles in na{\"i}ve/challenged fish. As expected, the bacterial burden in the spleen of na{\"i}ve fish increased over time and was correlated with the spleen index after Y. ruckeri challenge. The gene expression data showed that pro-inflammatory cytokines were upregulated post-infection in the spleen of both na{\"i}ve and vaccinated fish after Y. ruckeri challenge although the pro-inflammatory cytokine expression was much lower in vaccinated fish compared to the na{\"i}ve fish. A correlated expression between pro-inflammatory cytokines and anti-inflammatory cytokines was only seen in spleen of ERM vaccinated fish, where a Th1-like response was indicated by the correlated gene expression of IFN-γ, T-bet and IL-2. In contrast, in the gills, the inflammatory gene response was enhanced in vaccinated fish compared to na{\"i}ve fish, but perhaps more importantly there was a strong upregulation of IL-22 which was negatively correlated with IFN-γ gene expression at this site. Thus, it is possible that different types of adaptive responses are on-going within the vaccinated fish during infection with Y. ruckeri, potentially affected by the site and stage of infection.",
keywords = "animals, bacterial vaccines, cytokines, fish diseases, gene expression profiling, gene expression regulation, gills, Oncorhynchus mykiss, spleen, vaccination, yersinia infections, Yersinia ruckeri",
author = "Harun, {Nor Omaima} and Tiehui Wang and Secombes, {Chris J}",
note = "Copyright {\circledC} 2011 Elsevier Ltd. All rights reserved.",
year = "2011",
month = "6",
day = "10",
doi = "10.1016/j.vaccine.2011.04.003",
language = "English",
volume = "29",
pages = "4388--4399",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "26",

}

TY - JOUR

T1 - Gene expression profiling in naïve and vaccinated rainbow trout after Yersinia ruckeri infection

T2 - insights into the mechanisms of protection seen in vaccinated fish

AU - Harun, Nor Omaima

AU - Wang, Tiehui

AU - Secombes, Chris J

N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.

PY - 2011/6/10

Y1 - 2011/6/10

N2 - Despite the importance and success of vaccination against bacterial diseases in fish, little is known about the mechanisms of vaccine-induced disease resistance. In this study a known efficacious bacterial vaccine, to Enteric Redmouth Disease (ERM), was used to vaccinate rainbow trout, and sixty days later the fish were challenged with the causative agent of the disease, Yersinia ruckeri. The bacterial burden in the spleen, the spleen index, and the expression profiles of pro- and anti-inflammatory cytokines and marker genes for T helper (Th) cells in the spleen and gills were analyzed, in comparison to the profiles in naïve/challenged fish. As expected, the bacterial burden in the spleen of naïve fish increased over time and was correlated with the spleen index after Y. ruckeri challenge. The gene expression data showed that pro-inflammatory cytokines were upregulated post-infection in the spleen of both naïve and vaccinated fish after Y. ruckeri challenge although the pro-inflammatory cytokine expression was much lower in vaccinated fish compared to the naïve fish. A correlated expression between pro-inflammatory cytokines and anti-inflammatory cytokines was only seen in spleen of ERM vaccinated fish, where a Th1-like response was indicated by the correlated gene expression of IFN-γ, T-bet and IL-2. In contrast, in the gills, the inflammatory gene response was enhanced in vaccinated fish compared to naïve fish, but perhaps more importantly there was a strong upregulation of IL-22 which was negatively correlated with IFN-γ gene expression at this site. Thus, it is possible that different types of adaptive responses are on-going within the vaccinated fish during infection with Y. ruckeri, potentially affected by the site and stage of infection.

AB - Despite the importance and success of vaccination against bacterial diseases in fish, little is known about the mechanisms of vaccine-induced disease resistance. In this study a known efficacious bacterial vaccine, to Enteric Redmouth Disease (ERM), was used to vaccinate rainbow trout, and sixty days later the fish were challenged with the causative agent of the disease, Yersinia ruckeri. The bacterial burden in the spleen, the spleen index, and the expression profiles of pro- and anti-inflammatory cytokines and marker genes for T helper (Th) cells in the spleen and gills were analyzed, in comparison to the profiles in naïve/challenged fish. As expected, the bacterial burden in the spleen of naïve fish increased over time and was correlated with the spleen index after Y. ruckeri challenge. The gene expression data showed that pro-inflammatory cytokines were upregulated post-infection in the spleen of both naïve and vaccinated fish after Y. ruckeri challenge although the pro-inflammatory cytokine expression was much lower in vaccinated fish compared to the naïve fish. A correlated expression between pro-inflammatory cytokines and anti-inflammatory cytokines was only seen in spleen of ERM vaccinated fish, where a Th1-like response was indicated by the correlated gene expression of IFN-γ, T-bet and IL-2. In contrast, in the gills, the inflammatory gene response was enhanced in vaccinated fish compared to naïve fish, but perhaps more importantly there was a strong upregulation of IL-22 which was negatively correlated with IFN-γ gene expression at this site. Thus, it is possible that different types of adaptive responses are on-going within the vaccinated fish during infection with Y. ruckeri, potentially affected by the site and stage of infection.

KW - animals

KW - bacterial vaccines

KW - cytokines

KW - fish diseases

KW - gene expression profiling

KW - gene expression regulation

KW - gills

KW - Oncorhynchus mykiss

KW - spleen

KW - vaccination

KW - yersinia infections

KW - Yersinia ruckeri

U2 - 10.1016/j.vaccine.2011.04.003

DO - 10.1016/j.vaccine.2011.04.003

M3 - Article

VL - 29

SP - 4388

EP - 4399

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 26

ER -