Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors

Georgia Schäfer, Gordon Douglas Brown

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Macrophages, tissue-based phagocytic cells derived from blood monocytes, play important roles in immunity and homeostasis. As professional scavengers, macrophages phagocytose microbes, apoptotic and necrotic cells and take up modified lipoprotein particles. However, many of their complex interactions with other immune cells and/or various ligands are not yet clearly understood. To identify and isolate macrophage cell surface molecules, particularly macrophage receptors, for which ligands are known, a powerful generalized screening method has been established. As discussed in this chapter, this technique based on function has been successfully applied for the identification of dectin-1, the major macrophage receptor involved in the binding and recognition of beta-glucans (Nature 413:36-37, 2001).
Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalMethods in Molecular Biology
Volume531
DOIs
Publication statusPublished - 2009

Fingerprint

Gene Library
Macrophages
Ligands
beta-Glucans
Phagocytes
Phagocytosis
Lipoproteins
Monocytes
Immunity
Homeostasis

Keywords

  • animals
  • fluorescein-5-isothiocyanate
  • gene library
  • macrophages
  • mice
  • molecular biology
  • NIH 3T3 cells
  • receptors, cell surface
  • retroviridae

Cite this

Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors. / Schäfer, Georgia; Brown, Gordon Douglas.

In: Methods in Molecular Biology, Vol. 531, 2009, p. 1-15.

Research output: Contribution to journalArticle

@article{d75f5824b1f140ff9677e4253202ddb0,
title = "Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors",
abstract = "Macrophages, tissue-based phagocytic cells derived from blood monocytes, play important roles in immunity and homeostasis. As professional scavengers, macrophages phagocytose microbes, apoptotic and necrotic cells and take up modified lipoprotein particles. However, many of their complex interactions with other immune cells and/or various ligands are not yet clearly understood. To identify and isolate macrophage cell surface molecules, particularly macrophage receptors, for which ligands are known, a powerful generalized screening method has been established. As discussed in this chapter, this technique based on function has been successfully applied for the identification of dectin-1, the major macrophage receptor involved in the binding and recognition of beta-glucans (Nature 413:36-37, 2001).",
keywords = "animals, fluorescein-5-isothiocyanate, gene library, macrophages, mice, molecular biology, NIH 3T3 cells, receptors, cell surface, retroviridae",
author = "Georgia Sch{\"a}fer and Brown, {Gordon Douglas}",
year = "2009",
doi = "10.1007/978-1-59745-396-7_1",
language = "English",
volume = "531",
pages = "1--15",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors

AU - Schäfer, Georgia

AU - Brown, Gordon Douglas

PY - 2009

Y1 - 2009

N2 - Macrophages, tissue-based phagocytic cells derived from blood monocytes, play important roles in immunity and homeostasis. As professional scavengers, macrophages phagocytose microbes, apoptotic and necrotic cells and take up modified lipoprotein particles. However, many of their complex interactions with other immune cells and/or various ligands are not yet clearly understood. To identify and isolate macrophage cell surface molecules, particularly macrophage receptors, for which ligands are known, a powerful generalized screening method has been established. As discussed in this chapter, this technique based on function has been successfully applied for the identification of dectin-1, the major macrophage receptor involved in the binding and recognition of beta-glucans (Nature 413:36-37, 2001).

AB - Macrophages, tissue-based phagocytic cells derived from blood monocytes, play important roles in immunity and homeostasis. As professional scavengers, macrophages phagocytose microbes, apoptotic and necrotic cells and take up modified lipoprotein particles. However, many of their complex interactions with other immune cells and/or various ligands are not yet clearly understood. To identify and isolate macrophage cell surface molecules, particularly macrophage receptors, for which ligands are known, a powerful generalized screening method has been established. As discussed in this chapter, this technique based on function has been successfully applied for the identification of dectin-1, the major macrophage receptor involved in the binding and recognition of beta-glucans (Nature 413:36-37, 2001).

KW - animals

KW - fluorescein-5-isothiocyanate

KW - gene library

KW - macrophages

KW - mice

KW - molecular biology

KW - NIH 3T3 cells

KW - receptors, cell surface

KW - retroviridae

U2 - 10.1007/978-1-59745-396-7_1

DO - 10.1007/978-1-59745-396-7_1

M3 - Article

C2 - 19347307

VL - 531

SP - 1

EP - 15

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -