Genetic determinants of weight of fast- and slow-twitch skeletal muscle in 500-day-old mice of the C57BL/6J and DBA/2J lineage

Arimantas Lionikas, D A Blizard, G S Gerhard, D J Vandenbergh, J T Stout, G P Vogler, G E McClearn, L Larsson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

C57BL/6J (B6) and DBA/2J (D2) strains and two derivative populations, BXD recombinant inbred strains (BXD RIs) and B6D2F2, were used to explore genetic basis for variation in muscle weight at 500 days of age. In parallel with findings in 200-day-old mice (Lionikas A, Blizard DA, Vandenbergh DJ, Glover MG, Stout JT, Vogler GP, McClearn GE, and Larsson L. Physiol Genomics 16: 141-152, 2003), weight of slow-twitch soleus, mixed gastrocnemius, and fast-twitch tibialis anterior (TA) and extensor digitorum longus (EDL) muscles was 13-22% greater (P <0.001) in B6 than in D2. Distribution of BXD RI strain means indicated that genetic influence on muscle weight (strain effect P <0.001, all muscles) was of polygenic origin, and effect of genetic factors differed between males and females (strain-by-sex interaction: P <0.01 for soleus, EDL; P <0.05 for TA, gastrocnemius). Linkage analyses in B6D2F2 population identified QTL affecting muscle weight on Chr 1, 2, 6, and 9. Pleiotropic influences were observed for QTL on Chr 1 (soleus, TA), 2 (TA, EDL, gastrocnemius), and 9 (soleus, TA, EDL) and were not related to muscle type (fast/slow-twitch) or function (flexor/extensor). Effect of QTL on Chr 9 on soleus muscle was male specific. QTL on Chr 2 and 6 were previously observed at 200 days of age, whereas QTL on Chr 1 and 9 are novel muscle weight QTL. In summary, muscle weight in B6/D2 lineage is affected by a polygenic system that has variable influences at different ages, between males and females, and across muscles in a manner independent of muscle type.
Original languageEnglish
Pages (from-to)184-192
Number of pages9
JournalPhysiological Genomics
Volume21
Issue number2
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Inbred DBA Mouse
Inbred C57BL Mouse
Skeletal Muscle
Weights and Measures
Muscles
Genomics
Population

Keywords

  • Aging
  • Animals
  • Epistasis, Genetic
  • Female
  • Lod Score
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Muscle Fibers, Fast-Twitch
  • Muscle Fibers, Slow-Twitch
  • Muscle, Skeletal
  • Organ Size
  • Phenotype
  • Quantitative Trait Loci

Cite this

Genetic determinants of weight of fast- and slow-twitch skeletal muscle in 500-day-old mice of the C57BL/6J and DBA/2J lineage. / Lionikas, Arimantas; Blizard, D A; Gerhard, G S; Vandenbergh, D J; Stout, J T; Vogler, G P; McClearn, G E; Larsson, L.

In: Physiological Genomics, Vol. 21, No. 2, 04.2005, p. 184-192.

Research output: Contribution to journalArticle

Lionikas, Arimantas ; Blizard, D A ; Gerhard, G S ; Vandenbergh, D J ; Stout, J T ; Vogler, G P ; McClearn, G E ; Larsson, L. / Genetic determinants of weight of fast- and slow-twitch skeletal muscle in 500-day-old mice of the C57BL/6J and DBA/2J lineage. In: Physiological Genomics. 2005 ; Vol. 21, No. 2. pp. 184-192.
@article{c383a52e58db4fb28cba5c494ccf5112,
title = "Genetic determinants of weight of fast- and slow-twitch skeletal muscle in 500-day-old mice of the C57BL/6J and DBA/2J lineage",
abstract = "C57BL/6J (B6) and DBA/2J (D2) strains and two derivative populations, BXD recombinant inbred strains (BXD RIs) and B6D2F2, were used to explore genetic basis for variation in muscle weight at 500 days of age. In parallel with findings in 200-day-old mice (Lionikas A, Blizard DA, Vandenbergh DJ, Glover MG, Stout JT, Vogler GP, McClearn GE, and Larsson L. Physiol Genomics 16: 141-152, 2003), weight of slow-twitch soleus, mixed gastrocnemius, and fast-twitch tibialis anterior (TA) and extensor digitorum longus (EDL) muscles was 13-22{\%} greater (P <0.001) in B6 than in D2. Distribution of BXD RI strain means indicated that genetic influence on muscle weight (strain effect P <0.001, all muscles) was of polygenic origin, and effect of genetic factors differed between males and females (strain-by-sex interaction: P <0.01 for soleus, EDL; P <0.05 for TA, gastrocnemius). Linkage analyses in B6D2F2 population identified QTL affecting muscle weight on Chr 1, 2, 6, and 9. Pleiotropic influences were observed for QTL on Chr 1 (soleus, TA), 2 (TA, EDL, gastrocnemius), and 9 (soleus, TA, EDL) and were not related to muscle type (fast/slow-twitch) or function (flexor/extensor). Effect of QTL on Chr 9 on soleus muscle was male specific. QTL on Chr 2 and 6 were previously observed at 200 days of age, whereas QTL on Chr 1 and 9 are novel muscle weight QTL. In summary, muscle weight in B6/D2 lineage is affected by a polygenic system that has variable influences at different ages, between males and females, and across muscles in a manner independent of muscle type.",
keywords = "Aging, Animals, Epistasis, Genetic, Female, Lod Score, Male, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Muscle, Skeletal, Organ Size, Phenotype, Quantitative Trait Loci",
author = "Arimantas Lionikas and Blizard, {D A} and Gerhard, {G S} and Vandenbergh, {D J} and Stout, {J T} and Vogler, {G P} and McClearn, {G E} and L Larsson",
year = "2005",
month = "4",
doi = "10.1152/physiolgenomics.00209.2004",
language = "English",
volume = "21",
pages = "184--192",
journal = "Physiological Genomics",
issn = "1094-8341",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Genetic determinants of weight of fast- and slow-twitch skeletal muscle in 500-day-old mice of the C57BL/6J and DBA/2J lineage

AU - Lionikas, Arimantas

AU - Blizard, D A

AU - Gerhard, G S

AU - Vandenbergh, D J

AU - Stout, J T

AU - Vogler, G P

AU - McClearn, G E

AU - Larsson, L

PY - 2005/4

Y1 - 2005/4

N2 - C57BL/6J (B6) and DBA/2J (D2) strains and two derivative populations, BXD recombinant inbred strains (BXD RIs) and B6D2F2, were used to explore genetic basis for variation in muscle weight at 500 days of age. In parallel with findings in 200-day-old mice (Lionikas A, Blizard DA, Vandenbergh DJ, Glover MG, Stout JT, Vogler GP, McClearn GE, and Larsson L. Physiol Genomics 16: 141-152, 2003), weight of slow-twitch soleus, mixed gastrocnemius, and fast-twitch tibialis anterior (TA) and extensor digitorum longus (EDL) muscles was 13-22% greater (P <0.001) in B6 than in D2. Distribution of BXD RI strain means indicated that genetic influence on muscle weight (strain effect P <0.001, all muscles) was of polygenic origin, and effect of genetic factors differed between males and females (strain-by-sex interaction: P <0.01 for soleus, EDL; P <0.05 for TA, gastrocnemius). Linkage analyses in B6D2F2 population identified QTL affecting muscle weight on Chr 1, 2, 6, and 9. Pleiotropic influences were observed for QTL on Chr 1 (soleus, TA), 2 (TA, EDL, gastrocnemius), and 9 (soleus, TA, EDL) and were not related to muscle type (fast/slow-twitch) or function (flexor/extensor). Effect of QTL on Chr 9 on soleus muscle was male specific. QTL on Chr 2 and 6 were previously observed at 200 days of age, whereas QTL on Chr 1 and 9 are novel muscle weight QTL. In summary, muscle weight in B6/D2 lineage is affected by a polygenic system that has variable influences at different ages, between males and females, and across muscles in a manner independent of muscle type.

AB - C57BL/6J (B6) and DBA/2J (D2) strains and two derivative populations, BXD recombinant inbred strains (BXD RIs) and B6D2F2, were used to explore genetic basis for variation in muscle weight at 500 days of age. In parallel with findings in 200-day-old mice (Lionikas A, Blizard DA, Vandenbergh DJ, Glover MG, Stout JT, Vogler GP, McClearn GE, and Larsson L. Physiol Genomics 16: 141-152, 2003), weight of slow-twitch soleus, mixed gastrocnemius, and fast-twitch tibialis anterior (TA) and extensor digitorum longus (EDL) muscles was 13-22% greater (P <0.001) in B6 than in D2. Distribution of BXD RI strain means indicated that genetic influence on muscle weight (strain effect P <0.001, all muscles) was of polygenic origin, and effect of genetic factors differed between males and females (strain-by-sex interaction: P <0.01 for soleus, EDL; P <0.05 for TA, gastrocnemius). Linkage analyses in B6D2F2 population identified QTL affecting muscle weight on Chr 1, 2, 6, and 9. Pleiotropic influences were observed for QTL on Chr 1 (soleus, TA), 2 (TA, EDL, gastrocnemius), and 9 (soleus, TA, EDL) and were not related to muscle type (fast/slow-twitch) or function (flexor/extensor). Effect of QTL on Chr 9 on soleus muscle was male specific. QTL on Chr 2 and 6 were previously observed at 200 days of age, whereas QTL on Chr 1 and 9 are novel muscle weight QTL. In summary, muscle weight in B6/D2 lineage is affected by a polygenic system that has variable influences at different ages, between males and females, and across muscles in a manner independent of muscle type.

KW - Aging

KW - Animals

KW - Epistasis, Genetic

KW - Female

KW - Lod Score

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Inbred DBA

KW - Muscle Fibers, Fast-Twitch

KW - Muscle Fibers, Slow-Twitch

KW - Muscle, Skeletal

KW - Organ Size

KW - Phenotype

KW - Quantitative Trait Loci

U2 - 10.1152/physiolgenomics.00209.2004

DO - 10.1152/physiolgenomics.00209.2004

M3 - Article

VL - 21

SP - 184

EP - 192

JO - Physiological Genomics

JF - Physiological Genomics

SN - 1094-8341

IS - 2

ER -