Genome-wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β-catenin paradigm

Yukio Nakamura, Stefan Hoppler

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)
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Wnt/β-catenin signaling is an important cell-to-cell signaling mechanism that
controls gene expression during embryonic development and is critically
implicated in human diseases. Developmental, cellular, and transcriptional
responses to Wnt signaling are remarkably context-specific in different
biological processes. While nuclear localization of β-catenin is the key to
activation of the Wnt/β-catenin pathway and target gene expression, the
molecular mechanisms of how the same Wnt/β-catenin signaling pathway
induces specific responses remain undetermined. Recent advances in highthroughput
sequencing technologies and the availability of genome
information for Xenopus tropicalis have enabled us to uncover a genome-wide
view of Wnt/β-catenin signaling in early vertebrate embryos, which challenges
previous concepts about molecular mechanisms of Wnt target gene regulation.
In this review, we summarize our experimental approaches, introduce the
technologies we employed and focus on recent findings about Wnt target
gene regulation from Xenopus research. We will also discuss potential
functions of widespread β-catenin binding in the genome that we discovered
in this species.
Original languageEnglish
Article numbere22991
Issue number1-2
Early online date29 Jan 2017
Publication statusPublished - Jan 2017


  • Wnt signaling
  • β-Catenin
  • Xenopus
  • gastrula
  • ChIP-seq
  • RNA-seq
  • Amphibian
  • Organism, early development
  • Process, signalling
  • Process, transcription
  • Process, mesoderm
  • Tissue


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