Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection

Vishal Gupta; Ravi S. Baghel; Manoj Kumar; Puja Kumari; Vaibhav A. Mantri; C. R.K. Reddy; Bhavnath Jha

doi:10.1016/j.aquaculture.2011.06.009

Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection

Vishal Gupta, Ravi S. Baghel, Manoj Kumar, Puja Kumari, Vaibhav A. Mantri, C. R.K. Reddy^*, Bhavnath Jha

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The characteristics of agarose and growth for three isomorphic life phases of G. dura with their bio-molecular marker assisted selection have been described in this study. The tetrasporophyte showed superior quality of agarose over gametophytes and recorded growth rate was highest for females. The genetic relatedness studied with ISSR markers showed quadratic line of correlation between these phases (R²=1). Their genetic diversity determinants as percentage of polymorphic loci (PPL), average heterozygosity (He) and Shannon's Weaver index (I) were 55.55%, 0.5±0.07 and 0.33 respectively. The cytological analysis for chromosome count revealed 8 chromosomes in haploid gametophytic thallus (N) and 16 for diploid tetrasporophyte (2N) together with genetic structure analysis confirmed to their sexual mating behaviour. Their marker assisted selection based on ISSR generated characteristic band of 430bp specific to male, 860bp for female and two bands of 800 and 1600bp for tetrasporophytic thallus from primer 'A'. Similarly ISSR primer 'E' also generated bands specific to male, female and tetrasporophytes while others gave bands specific to either of life phase. Interestingly, endogenic ABA content was significantly higher for haploid gametophytes (female more than male) than diploid tetrasporophyte while no significant difference was observed in IBA content. Thus the study described not only the features of three life phases of G. dura but also reliable biomarkers for differentiating such isomorphic life phases which could be beneficial for the selection of cultivar and in breeding programmes.

Original language	English
Pages (from-to)	389-396
Number of pages	8
Journal	Aquaculture
Volume	318
Issue number	3-4
DOIs	https://doi.org/10.1016/j.aquaculture.2011.06.009
Publication status	Published - 8 Aug 2011

Bibliographical note

Funding Information:
We would like to thank Dr. Kamlesh Prasad for the characterisation of agarose. The financial support received from CSIR ( NWP-018 ) is gratefully acknowledged. Mr Manoj and Miss Puja would like to thank CSIR, New Delhi for awarding the Senior and Junior Research Fellowships respectively. We would like to thank the section editor of Aquaculture and reviewers for their valuable comments and suggestions while reviewing this manuscript.

Keywords

Agarose
Chromosome
Gracilaria dura
Life cycle stage
Marker assisted selection

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10.1016/j.aquaculture.2011.06.009

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@article{cde92096e9de4d15888c2d20c917d3f3,

title = "Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection",

abstract = "The characteristics of agarose and growth for three isomorphic life phases of G. dura with their bio-molecular marker assisted selection have been described in this study. The tetrasporophyte showed superior quality of agarose over gametophytes and recorded growth rate was highest for females. The genetic relatedness studied with ISSR markers showed quadratic line of correlation between these phases (R2=1). Their genetic diversity determinants as percentage of polymorphic loci (PPL), average heterozygosity (He) and Shannon's Weaver index (I) were 55.55%, 0.5±0.07 and 0.33 respectively. The cytological analysis for chromosome count revealed 8 chromosomes in haploid gametophytic thallus (N) and 16 for diploid tetrasporophyte (2N) together with genetic structure analysis confirmed to their sexual mating behaviour. Their marker assisted selection based on ISSR generated characteristic band of 430bp specific to male, 860bp for female and two bands of 800 and 1600bp for tetrasporophytic thallus from primer 'A'. Similarly ISSR primer 'E' also generated bands specific to male, female and tetrasporophytes while others gave bands specific to either of life phase. Interestingly, endogenic ABA content was significantly higher for haploid gametophytes (female more than male) than diploid tetrasporophyte while no significant difference was observed in IBA content. Thus the study described not only the features of three life phases of G. dura but also reliable biomarkers for differentiating such isomorphic life phases which could be beneficial for the selection of cultivar and in breeding programmes.",

keywords = "Agarose, Chromosome, Gracilaria dura, Life cycle stage, Marker assisted selection",

author = "Vishal Gupta and Baghel, {Ravi S.} and Manoj Kumar and Puja Kumari and Mantri, {Vaibhav A.} and Reddy, {C. R.K.} and Bhavnath Jha",

note = "Funding Information: We would like to thank Dr. Kamlesh Prasad for the characterisation of agarose. The financial support received from CSIR ( NWP-018 ) is gratefully acknowledged. Mr Manoj and Miss Puja would like to thank CSIR, New Delhi for awarding the Senior and Junior Research Fellowships respectively. We would like to thank the section editor of Aquaculture and reviewers for their valuable comments and suggestions while reviewing this manuscript.",

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doi = "10.1016/j.aquaculture.2011.06.009",

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journal = "Aquaculture",

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AU - Kumari, Puja

AU - Mantri, Vaibhav A.

AU - Reddy, C. R.K.

AU - Jha, Bhavnath

N1 - Funding Information: We would like to thank Dr. Kamlesh Prasad for the characterisation of agarose. The financial support received from CSIR ( NWP-018 ) is gratefully acknowledged. Mr Manoj and Miss Puja would like to thank CSIR, New Delhi for awarding the Senior and Junior Research Fellowships respectively. We would like to thank the section editor of Aquaculture and reviewers for their valuable comments and suggestions while reviewing this manuscript.

PY - 2011/8/8

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N2 - The characteristics of agarose and growth for three isomorphic life phases of G. dura with their bio-molecular marker assisted selection have been described in this study. The tetrasporophyte showed superior quality of agarose over gametophytes and recorded growth rate was highest for females. The genetic relatedness studied with ISSR markers showed quadratic line of correlation between these phases (R2=1). Their genetic diversity determinants as percentage of polymorphic loci (PPL), average heterozygosity (He) and Shannon's Weaver index (I) were 55.55%, 0.5±0.07 and 0.33 respectively. The cytological analysis for chromosome count revealed 8 chromosomes in haploid gametophytic thallus (N) and 16 for diploid tetrasporophyte (2N) together with genetic structure analysis confirmed to their sexual mating behaviour. Their marker assisted selection based on ISSR generated characteristic band of 430bp specific to male, 860bp for female and two bands of 800 and 1600bp for tetrasporophytic thallus from primer 'A'. Similarly ISSR primer 'E' also generated bands specific to male, female and tetrasporophytes while others gave bands specific to either of life phase. Interestingly, endogenic ABA content was significantly higher for haploid gametophytes (female more than male) than diploid tetrasporophyte while no significant difference was observed in IBA content. Thus the study described not only the features of three life phases of G. dura but also reliable biomarkers for differentiating such isomorphic life phases which could be beneficial for the selection of cultivar and in breeding programmes.

AB - The characteristics of agarose and growth for three isomorphic life phases of G. dura with their bio-molecular marker assisted selection have been described in this study. The tetrasporophyte showed superior quality of agarose over gametophytes and recorded growth rate was highest for females. The genetic relatedness studied with ISSR markers showed quadratic line of correlation between these phases (R2=1). Their genetic diversity determinants as percentage of polymorphic loci (PPL), average heterozygosity (He) and Shannon's Weaver index (I) were 55.55%, 0.5±0.07 and 0.33 respectively. The cytological analysis for chromosome count revealed 8 chromosomes in haploid gametophytic thallus (N) and 16 for diploid tetrasporophyte (2N) together with genetic structure analysis confirmed to their sexual mating behaviour. Their marker assisted selection based on ISSR generated characteristic band of 430bp specific to male, 860bp for female and two bands of 800 and 1600bp for tetrasporophytic thallus from primer 'A'. Similarly ISSR primer 'E' also generated bands specific to male, female and tetrasporophytes while others gave bands specific to either of life phase. Interestingly, endogenic ABA content was significantly higher for haploid gametophytes (female more than male) than diploid tetrasporophyte while no significant difference was observed in IBA content. Thus the study described not only the features of three life phases of G. dura but also reliable biomarkers for differentiating such isomorphic life phases which could be beneficial for the selection of cultivar and in breeding programmes.

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KW - Chromosome

KW - Gracilaria dura

KW - Life cycle stage

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DO - 10.1016/j.aquaculture.2011.06.009

M3 - Article

AN - SCOPUS:79960139060

SN - 0044-8486

VL - 318

SP - 389

EP - 396

JO - Aquaculture

JF - Aquaculture

IS - 3-4

ER -

Growth and agarose characteristics of isomorphic gametophyte (male and female) and sporophyte of Gracilaria dura and their marker assisted selection

Abstract

Bibliographical note

Keywords

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