Growth inhibition of Escherichia coli by dichloromethane in cells expressing dichloromethane dehalogenase/glutathione S-transferase

G J Evans, G P Ferguson, I R Booth, S Vuilleumier

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Dichloromethane (DCM) dehalogenase converts DCM to formaldehyde via the formation of glutathione metabolites and generates 2 mol HCI per mol DCM formation or glutathione metabolites and generates 2 mol HCI per mol DCM metabolized. Growth of Escherichia coli expressing DCM dehalogenase was immediately and severely inhibited during conversion of 0.3 mM DCM. Intracellular pH (pH(i)) rapidly decreased and chloride ions were steadily released into the medium. Bacterial growth resumed after completion of DCM conversion and cell viability was unaffected. At 0.6 mM DCM there was no recovery from growth inhibition in liquid culture due to the build-up of inhibitory concentrations of formaldehyde. DCM turnover stimulated potassium efflux from cells, which was suppressed by glucose. The potassium efflux, therefore, did not contribute to growth inhibition. It was concluded that initial growth inhibition results from lowering of the cytoplasmic pH. but severity of growth inhibition was greater than expected for the change in pH(i). Possible contributors to growth inhibition are discussed.

Original languageEnglish
Pages (from-to)2967-2975
Number of pages9
JournalMicrobiology
Volume146
Publication statusPublished - 2000

Keywords

  • glutathione
  • dichloromethane
  • intracellular pH
  • chloride
  • formaldehyde
  • DEPENDENT FORMALDEHYDE DEHYDROGENASE
  • POTASSIUM CHANNELS
  • GLUTATHIONE
  • ACTIVATION
  • BACTERIAL
  • SURVIVAL
  • K+
  • METHYLGLYOXAL
  • SALMONELLA
  • EFFLUX

Cite this

Growth inhibition of Escherichia coli by dichloromethane in cells expressing dichloromethane dehalogenase/glutathione S-transferase. / Evans, G J ; Ferguson, G P ; Booth, I R ; Vuilleumier, S .

In: Microbiology , Vol. 146, 2000, p. 2967-2975.

Research output: Contribution to journalArticle

Evans, G J ; Ferguson, G P ; Booth, I R ; Vuilleumier, S . / Growth inhibition of Escherichia coli by dichloromethane in cells expressing dichloromethane dehalogenase/glutathione S-transferase. In: Microbiology . 2000 ; Vol. 146. pp. 2967-2975.
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T1 - Growth inhibition of Escherichia coli by dichloromethane in cells expressing dichloromethane dehalogenase/glutathione S-transferase

AU - Evans, G J

AU - Ferguson, G P

AU - Booth, I R

AU - Vuilleumier, S

PY - 2000

Y1 - 2000

N2 - Dichloromethane (DCM) dehalogenase converts DCM to formaldehyde via the formation of glutathione metabolites and generates 2 mol HCI per mol DCM formation or glutathione metabolites and generates 2 mol HCI per mol DCM metabolized. Growth of Escherichia coli expressing DCM dehalogenase was immediately and severely inhibited during conversion of 0.3 mM DCM. Intracellular pH (pH(i)) rapidly decreased and chloride ions were steadily released into the medium. Bacterial growth resumed after completion of DCM conversion and cell viability was unaffected. At 0.6 mM DCM there was no recovery from growth inhibition in liquid culture due to the build-up of inhibitory concentrations of formaldehyde. DCM turnover stimulated potassium efflux from cells, which was suppressed by glucose. The potassium efflux, therefore, did not contribute to growth inhibition. It was concluded that initial growth inhibition results from lowering of the cytoplasmic pH. but severity of growth inhibition was greater than expected for the change in pH(i). Possible contributors to growth inhibition are discussed.

AB - Dichloromethane (DCM) dehalogenase converts DCM to formaldehyde via the formation of glutathione metabolites and generates 2 mol HCI per mol DCM formation or glutathione metabolites and generates 2 mol HCI per mol DCM metabolized. Growth of Escherichia coli expressing DCM dehalogenase was immediately and severely inhibited during conversion of 0.3 mM DCM. Intracellular pH (pH(i)) rapidly decreased and chloride ions were steadily released into the medium. Bacterial growth resumed after completion of DCM conversion and cell viability was unaffected. At 0.6 mM DCM there was no recovery from growth inhibition in liquid culture due to the build-up of inhibitory concentrations of formaldehyde. DCM turnover stimulated potassium efflux from cells, which was suppressed by glucose. The potassium efflux, therefore, did not contribute to growth inhibition. It was concluded that initial growth inhibition results from lowering of the cytoplasmic pH. but severity of growth inhibition was greater than expected for the change in pH(i). Possible contributors to growth inhibition are discussed.

KW - glutathione

KW - dichloromethane

KW - intracellular pH

KW - chloride

KW - formaldehyde

KW - DEPENDENT FORMALDEHYDE DEHYDROGENASE

KW - POTASSIUM CHANNELS

KW - GLUTATHIONE

KW - ACTIVATION

KW - BACTERIAL

KW - SURVIVAL

KW - K+

KW - METHYLGLYOXAL

KW - SALMONELLA

KW - EFFLUX

M3 - Article

VL - 146

SP - 2967

EP - 2975

JO - Microbiology

JF - Microbiology

SN - 1350-0872

ER -