Haplotypes defined by promoter and intron 1 polymorphisms of the COLIA1 gene regulate bone mineral density in women

Tracy L. Stewart, Huilin Jin, Fiona E. A. McGuigan, Omar M. E. Albagha, Natalia Garcia-Giralt, Amelia Bassiti, Daniel Grinberg, Susana Balcells, David M Reid, Stuart H. Ralston

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Abstract

Context: The COLIA1 gene is a strong candidate for susceptibility to osteoporosis. The causal genetic variants are currently unclear, but the most likely are functional polymorphisms in the promoter and intron 1 of COLIA1.
Objective: The objective of the study was to determine whether promoter and intron 1 polymorphisms of COLIA1 or haplotypes defined by these polymorphisms regulate bone mineral density (BMD) in women.
Design: This was a population-based association study involving 3270 women from the United Kingdom who took part in a regional osteoporosis screening program.
Main Outcome Measures: BMD at the lumbar spine (LS-BMD) and femoral neck (FN-BMD) was measured on two occasions approximately 6 yr apart, in relation to polymorphisms and haplotypes defined by polymorphisms within the COLIA1 intron 1 (+ 1245G/T; rs1800012) and promoter (-1997G/T; rs1107946; -1663IndelT; rs2412298).
Results: The polymorphisms were in strong linkage disequilibrium, and three haplotypes accounted for more than 95% of alleles at the COLIA1 locus. The individual polymorphisms were associated with BMD, but the most consistent associations were with haplotypes defined by all three polymorphisms. Homozygote carriers of haplotype 2 (-1997G/-1663delT/+1245T) had reduced BMD at baseline (P = 0.007 for LSBMD; P = 0.008 for FN-BMD), whereas homozygotes for haplotype 3 (-1997T/-1663insT/-1245G) had increased BMD (P = 0.007 for LSBMD). Similar associations were observed at follow-up for haplotype 3, but the association with haplotype 2 was weaker due to increased uptake of hormone replacement therapy in homozygotes for this haplotype.
Conclusions: Two haplotypes defined by polymorphisms in the 5' flank of the COLIA1 regulate BMD in a bidirectional manner in women.

Original languageEnglish
Pages (from-to)3575-3583
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume91
Issue number9
Early online date27 Jun 2006
DOIs
Publication statusPublished - 1 Sep 2006

Keywords

  • hormone replacement therapy
  • SP1 binding site
  • osteoporotic fractures
  • postmenopausal women
  • in vitro
  • alleles
  • association
  • risk
  • perimenopausal
  • susceptibility

Cite this

Stewart, T. L., Jin, H., McGuigan, F. E. A., Albagha, O. M. E., Garcia-Giralt, N., Bassiti, A., Grinberg, D., Balcells, S., Reid, D. M., & Ralston, S. H. (2006). Haplotypes defined by promoter and intron 1 polymorphisms of the COLIA1 gene regulate bone mineral density in women. Journal of Clinical Endocrinology and Metabolism, 91(9), 3575-3583. https://doi.org/10.1210/jc.2005-2651