HETEROLOGOUS EXPRESSION OF AN ENDOGLUCANASE GENE (ENDA) FROM THE RUMINAL ANAEROBE RUMINOCOCCUS-FLAVEFACIENS-17 IN STREPTOCOCCUS-BOVIS AND STREPTOCOCCUS-SANGUIS

T R WHITEHEAD, H J FLINT

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The heterologous expression of a cloned endoglucanase gene (endA) from the ruminal bacterium Ruminococcus flavefaciens 17 was demonstrated in the Streptococcus species S. bovis JB1 and S. sanguis DL1. The endA gene was introduced into S. bovis and S. sanguis using the Escherichia coli/Streptococcus shuttle vector pVA838. Expression of the gene was detected by clearing zones around the recombinant colonies on agar plates containing carboxymethylcellulose stained with Congo red. S. bovis JB1 containing the endA gene was capable of utilizing cellotetraose at a faster rate than the parent strain. This is the first demonstration that Streptococcus species can express a gene from a Ruminococcus flavefaciens strain.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalFEMS Microbiology Letters
Volume126
Issue number2
Publication statusPublished - 15 Feb 1995

Keywords

  • RUMINOCOCCUS FLAVEFACIENS
  • STREPTOCOCCUS BOVIS
  • ENDOGLUCANASE
  • STREPTOCOCCUS SANGUIS
  • BUTYRIVIBRIO-FIBRISOLVENS
  • CLONING
  • BACTERIA
  • ELECTROPORATION
  • FERMENTATION
  • SEQUENCE
  • DOMAINS
  • ENZYME
  • SYSTEM
  • ALBUS

Cite this

@article{32728bac4a7e47d3a449697f30d458c3,
title = "HETEROLOGOUS EXPRESSION OF AN ENDOGLUCANASE GENE (ENDA) FROM THE RUMINAL ANAEROBE RUMINOCOCCUS-FLAVEFACIENS-17 IN STREPTOCOCCUS-BOVIS AND STREPTOCOCCUS-SANGUIS",
abstract = "The heterologous expression of a cloned endoglucanase gene (endA) from the ruminal bacterium Ruminococcus flavefaciens 17 was demonstrated in the Streptococcus species S. bovis JB1 and S. sanguis DL1. The endA gene was introduced into S. bovis and S. sanguis using the Escherichia coli/Streptococcus shuttle vector pVA838. Expression of the gene was detected by clearing zones around the recombinant colonies on agar plates containing carboxymethylcellulose stained with Congo red. S. bovis JB1 containing the endA gene was capable of utilizing cellotetraose at a faster rate than the parent strain. This is the first demonstration that Streptococcus species can express a gene from a Ruminococcus flavefaciens strain.",
keywords = "RUMINOCOCCUS FLAVEFACIENS, STREPTOCOCCUS BOVIS, ENDOGLUCANASE, STREPTOCOCCUS SANGUIS, BUTYRIVIBRIO-FIBRISOLVENS, CLONING, BACTERIA, ELECTROPORATION, FERMENTATION, SEQUENCE, DOMAINS, ENZYME, SYSTEM, ALBUS",
author = "WHITEHEAD, {T R} and FLINT, {H J}",
year = "1995",
month = "2",
day = "15",
language = "English",
volume = "126",
pages = "165--169",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - HETEROLOGOUS EXPRESSION OF AN ENDOGLUCANASE GENE (ENDA) FROM THE RUMINAL ANAEROBE RUMINOCOCCUS-FLAVEFACIENS-17 IN STREPTOCOCCUS-BOVIS AND STREPTOCOCCUS-SANGUIS

AU - WHITEHEAD, T R

AU - FLINT, H J

PY - 1995/2/15

Y1 - 1995/2/15

N2 - The heterologous expression of a cloned endoglucanase gene (endA) from the ruminal bacterium Ruminococcus flavefaciens 17 was demonstrated in the Streptococcus species S. bovis JB1 and S. sanguis DL1. The endA gene was introduced into S. bovis and S. sanguis using the Escherichia coli/Streptococcus shuttle vector pVA838. Expression of the gene was detected by clearing zones around the recombinant colonies on agar plates containing carboxymethylcellulose stained with Congo red. S. bovis JB1 containing the endA gene was capable of utilizing cellotetraose at a faster rate than the parent strain. This is the first demonstration that Streptococcus species can express a gene from a Ruminococcus flavefaciens strain.

AB - The heterologous expression of a cloned endoglucanase gene (endA) from the ruminal bacterium Ruminococcus flavefaciens 17 was demonstrated in the Streptococcus species S. bovis JB1 and S. sanguis DL1. The endA gene was introduced into S. bovis and S. sanguis using the Escherichia coli/Streptococcus shuttle vector pVA838. Expression of the gene was detected by clearing zones around the recombinant colonies on agar plates containing carboxymethylcellulose stained with Congo red. S. bovis JB1 containing the endA gene was capable of utilizing cellotetraose at a faster rate than the parent strain. This is the first demonstration that Streptococcus species can express a gene from a Ruminococcus flavefaciens strain.

KW - RUMINOCOCCUS FLAVEFACIENS

KW - STREPTOCOCCUS BOVIS

KW - ENDOGLUCANASE

KW - STREPTOCOCCUS SANGUIS

KW - BUTYRIVIBRIO-FIBRISOLVENS

KW - CLONING

KW - BACTERIA

KW - ELECTROPORATION

KW - FERMENTATION

KW - SEQUENCE

KW - DOMAINS

KW - ENZYME

KW - SYSTEM

KW - ALBUS

M3 - Article

VL - 126

SP - 165

EP - 169

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -