High-performance liquid chromatographic determination of quercetin and isorhamnetin in rat tissues using beta-glucuronidase and acid hydrolysis

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Abstract

Quercetin is a plant polyphenol which is present in the diet as an aglycone and as sugar conjugates. Despite potent vasodilatory and antioxidant effects in vitro, destruction by intestinal organisms has been assumed to Limit its nutritional relevance in the rat. However, we have refined extraction techniques using P-glucuronidase followed by acid hydrolysis. Following this with HPLC methodology with post-column derivatisation, we have detected significant concentrations of quercetin and its metabolite, isorhamnetin, in tissues of rats maintained on quercetin-rich diets. Percentage recoveries are greater than 95% and intra-batch variation does not exceed 7% suggesting that the method may be useful in further studies of the biological role of this flavonoid. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)413-417
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume738
Issue number2
DOIs
Publication statusPublished - 11 Feb 2000

Keywords

  • quercetin
  • isorhamnetin
  • beta-glucuronidase
  • flavonols
  • plasma

Cite this

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title = "High-performance liquid chromatographic determination of quercetin and isorhamnetin in rat tissues using beta-glucuronidase and acid hydrolysis",
abstract = "Quercetin is a plant polyphenol which is present in the diet as an aglycone and as sugar conjugates. Despite potent vasodilatory and antioxidant effects in vitro, destruction by intestinal organisms has been assumed to Limit its nutritional relevance in the rat. However, we have refined extraction techniques using P-glucuronidase followed by acid hydrolysis. Following this with HPLC methodology with post-column derivatisation, we have detected significant concentrations of quercetin and its metabolite, isorhamnetin, in tissues of rats maintained on quercetin-rich diets. Percentage recoveries are greater than 95{\%} and intra-batch variation does not exceed 7{\%} suggesting that the method may be useful in further studies of the biological role of this flavonoid. (C) 2000 Elsevier Science B.V. All rights reserved.",
keywords = "quercetin, isorhamnetin, beta-glucuronidase, flavonols, plasma",
author = "Morrice, {Philip C.} and Sharon Wood and Duthie, {Garry G.}",
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T1 - High-performance liquid chromatographic determination of quercetin and isorhamnetin in rat tissues using beta-glucuronidase and acid hydrolysis

AU - Morrice, Philip C.

AU - Wood, Sharon

AU - Duthie, Garry G.

PY - 2000/2/11

Y1 - 2000/2/11

N2 - Quercetin is a plant polyphenol which is present in the diet as an aglycone and as sugar conjugates. Despite potent vasodilatory and antioxidant effects in vitro, destruction by intestinal organisms has been assumed to Limit its nutritional relevance in the rat. However, we have refined extraction techniques using P-glucuronidase followed by acid hydrolysis. Following this with HPLC methodology with post-column derivatisation, we have detected significant concentrations of quercetin and its metabolite, isorhamnetin, in tissues of rats maintained on quercetin-rich diets. Percentage recoveries are greater than 95% and intra-batch variation does not exceed 7% suggesting that the method may be useful in further studies of the biological role of this flavonoid. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - Quercetin is a plant polyphenol which is present in the diet as an aglycone and as sugar conjugates. Despite potent vasodilatory and antioxidant effects in vitro, destruction by intestinal organisms has been assumed to Limit its nutritional relevance in the rat. However, we have refined extraction techniques using P-glucuronidase followed by acid hydrolysis. Following this with HPLC methodology with post-column derivatisation, we have detected significant concentrations of quercetin and its metabolite, isorhamnetin, in tissues of rats maintained on quercetin-rich diets. Percentage recoveries are greater than 95% and intra-batch variation does not exceed 7% suggesting that the method may be useful in further studies of the biological role of this flavonoid. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - quercetin

KW - isorhamnetin

KW - beta-glucuronidase

KW - flavonols

KW - plasma

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DO - 10.1016/S0378-4347(99)00520-4

M3 - Article

VL - 738

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EP - 417

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JF - Journal of Chromatography B

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