Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

K Fliegerova, S Pazoutova, P Pristas, H J Flint

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Abstract

Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalPlasmid
Volume44
Issue number1
Publication statusPublished - Jul 2000

Keywords

  • COMPLETE NUCLEOTIDE-SEQUENCE
  • SMALL CRYPTIC PLASMIDS
  • ESCHERICHIA-COLI
  • REPLICATION
  • ALIGNMENT
  • REGION

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