Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

K Fliegerova, S Pazoutova, P Pristas, H J Flint

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalPlasmid
Volume44
Issue number1
Publication statusPublished - Jul 2000

Keywords

  • COMPLETE NUCLEOTIDE-SEQUENCE
  • SMALL CRYPTIC PLASMIDS
  • ESCHERICHIA-COLI
  • REPLICATION
  • ALIGNMENT
  • REGION

Cite this

Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium. / Fliegerova, K ; Pazoutova, S ; Pristas, P ; Flint, H J .

In: Plasmid, Vol. 44, No. 1, 07.2000, p. 94-99.

Research output: Contribution to journalArticle

Fliegerova, K, Pazoutova, S, Pristas, P & Flint, HJ 2000, 'Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium', Plasmid, vol. 44, no. 1, pp. 94-99.
Fliegerova, K ; Pazoutova, S ; Pristas, P ; Flint, H J . / Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium. In: Plasmid. 2000 ; Vol. 44, No. 1. pp. 94-99.
@article{e9406ba018b54558ad1fb238fb2e9c66,
title = "Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium",
abstract = "Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2{\%}. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75{\%}) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.",
keywords = "COMPLETE NUCLEOTIDE-SEQUENCE, SMALL CRYPTIC PLASMIDS, ESCHERICHIA-COLI, REPLICATION, ALIGNMENT, REGION",
author = "K Fliegerova and S Pazoutova and P Pristas and Flint, {H J}",
year = "2000",
month = "7",
language = "English",
volume = "44",
pages = "94--99",
journal = "Plasmid",
issn = "0147-619X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

AU - Fliegerova, K

AU - Pazoutova, S

AU - Pristas, P

AU - Flint, H J

PY - 2000/7

Y1 - 2000/7

N2 - Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

AB - Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

KW - COMPLETE NUCLEOTIDE-SEQUENCE

KW - SMALL CRYPTIC PLASMIDS

KW - ESCHERICHIA-COLI

KW - REPLICATION

KW - ALIGNMENT

KW - REGION

M3 - Article

VL - 44

SP - 94

EP - 99

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 1

ER -