Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method

Leo M.Y. Lee, Selina T.K. Tam, Peter J. McCaffery, Alisa S.W. Shum*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The effect of all-trans retinoic acid (RA) on embryogenesis is tissue specific and highly concentration dependent. Using a liquid chromatography/mass spectrometry-based method to quantify trace amounts of RA in embryonic tissue requires expensive specialist facilities. Here, we describe the use of a RA response element (RARE)-lacZ reporter cell-based method, which is simple and cost effective, to measure RA levels in small pieces of tissue from the embryo. We further apply this method to quantitatively assay activities of RA-synthesizing and RA-catabolizing enzymes, the key regulators of RA bioavailability in tissues and developing organs of the embryo.

Original languageEnglish
Title of host publicationRetinoid and Rexinoid Signaling
EditorsSwapan K. Ray
PublisherHUMANA PRESS INC
Pages181-192
Number of pages12
Volume2019
ISBN (Electronic)978-1-4939-9585-1
ISBN (Print)978-1-4939-9584-4
DOIs
Publication statusPublished - 30 Jul 2019

Publication series

NameMethods in Molecular Biology
Volume2019
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Tretinoin
Embryonic Structures
Response Elements
Liquid Chromatography
Biological Availability
Embryonic Development
Mass Spectrometry
Costs and Cost Analysis
Enzymes

Keywords

  • Cell culture
  • CYP26
  • Embryo
  • RALDH
  • Retinoic acid
  • Retinoic acid metabolism
  • Retinoic acid reporter cell line
  • Retinoic acid synthesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lee, L. M. Y., Tam, S. T. K., McCaffery, P. J., & Shum, A. S. W. (2019). Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method. In S. K. Ray (Ed.), Retinoid and Rexinoid Signaling (Vol. 2019, pp. 181-192). (Methods in Molecular Biology; Vol. 2019). HUMANA PRESS INC. https://doi.org/10.1007/978-1-4939-9585-1_13

Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method. / Lee, Leo M.Y.; Tam, Selina T.K.; McCaffery, Peter J.; Shum, Alisa S.W.

Retinoid and Rexinoid Signaling. ed. / Swapan K. Ray. Vol. 2019 HUMANA PRESS INC, 2019. p. 181-192 (Methods in Molecular Biology; Vol. 2019).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lee, LMY, Tam, STK, McCaffery, PJ & Shum, ASW 2019, Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method. in SK Ray (ed.), Retinoid and Rexinoid Signaling. vol. 2019, Methods in Molecular Biology, vol. 2019, HUMANA PRESS INC, pp. 181-192. https://doi.org/10.1007/978-1-4939-9585-1_13
Lee LMY, Tam STK, McCaffery PJ, Shum ASW. Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method. In Ray SK, editor, Retinoid and Rexinoid Signaling. Vol. 2019. HUMANA PRESS INC. 2019. p. 181-192. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9585-1_13
Lee, Leo M.Y. ; Tam, Selina T.K. ; McCaffery, Peter J. ; Shum, Alisa S.W. / Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method. Retinoid and Rexinoid Signaling. editor / Swapan K. Ray. Vol. 2019 HUMANA PRESS INC, 2019. pp. 181-192 (Methods in Molecular Biology).
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