Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation

Andrew S J Marshall, Janet A Willment, Elwira Pyz, Kevin M Dennehy, Delyth M Reid, Pietro Dri, Siamon Gordon, Simon Y C Wong, Gordon D Brown

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

C-type lectins are the most diverse and prevalent lectin family in immunity. Particular interest has recently been attracted by the C-type lectin-like receptors on NK cells, which appear to regulate the activation/inhibitory balance of these cells, controlling cytotoxicity and cytokine production. We previously identified a human C-type lectin-like receptor, closely related to both the beta-glucan receptor and the lectin-like receptor for oxidized-LDL, named MICL (myeloid inhibitory C-type lectin-like receptor), which we had shown using chimeric analysis to function as an inhibitory receptor. Using a novel MICL-specific monoclonal antibody, we show here that human MICL is expressed primarily on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. Although MICL was highly N-glycosylated in primary cells, the level of glycosylation was found to vary between cell types. MICL surface expression was down-regulated during inflammatory/activation conditions in vitro, as well as during an in vivo model of acute inflammation, which we characterize here. This suggests that human MICL may be involved in the control of myeloid cell activation during inflammation.

Original languageEnglish
Pages (from-to)2159-2169
Number of pages11
JournalEuropean Journal of Immunology
Volume36
Issue number8
Early online date12 Jul 2006
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Cells, Cultured
  • Dendritic Cells
  • Down-Regulation
  • Glycosylation
  • Humans
  • Inflammation
  • Lectins, C-Type
  • Macrophages
  • Mice
  • Receptors, Mitogen

Cite this

Marshall, A. S. J., Willment, J. A., Pyz, E., Dennehy, K. M., Reid, D. M., Dri, P., Gordon, S., Wong, S. Y. C., & Brown, G. D. (2006). Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation. European Journal of Immunology, 36(8), 2159-2169. https://doi.org/10.1002/eji.200535628