Hypothalamic neuropeptide gene expression during recovery from food restriction superimposed on short-day photoperiod-induced weight loss in the Siberian hamster

Zoë A. Archer, Kim-Marie Moar, Tracy Logie, Laura Reilly, Valerie Joan Stevens, Peter John Morgan, Julian Mercer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Previously, 40% food restriction of male Siberian hamsters over 21 days in short-day (SD) photoperiod induced characteristic changes in expression of hypothalamic arcuate nucleus energy balance genes; mRNAs for neuropeptide Y, agouti-related peptide, and leptin receptor were upregulated, and those of proopiomelanocortin and cocaine- and amphetamine-regulated transcript were depressed. The present study examined the effect of refeeding hamsters for 6 days (approximately 50% recovery of weight differential) or 19 days (resumption of appropriate weight trajectory). Hyperphagia continued throughout refeeding, but differences in fat pad weights and leptin levels had disappeared after 19 days. Cocaine- and amphetamine-regulated transcript gene expression was depressed by prior restriction in both refed groups. The depressive effect of prior restriction on proopiomelanocortin gene expression had disappeared after 19 days of refeeding. There was no effect of prior food restriction on neuropeptide Y or agouti-related peptide gene expression. Expression of the anorexigenic brain-derived neurotrophic factor was downregulated in the ventromedial nucleus after SD exposure for 12 wk. In the SD food restriction study, there were effects of photoperiod on brain-derived neurotrophic factor gene expression but not of prior food restriction. Hypothalamic energy balance genes in the hamster respond asynchronously to return to a seasonally appropriate body weight. The achievement of this weight rather than the weight at which caloric restriction was imposed is the critical factor. The differential responses of hypothalamic energy balance genes to food restriction and refeeding are poorly characterized in any species, a critical issue given their potential relevance to human weight loss strategies that involve caloric restriction.
Original languageEnglish
Pages (from-to)R1094-R1101
Number of pages8
JournalAmerican Journal of Physiology-Regulatory Integrative and Comparative Physiology
Volume293
Issue number3
DOIs
Publication statusPublished - Sep 2007

Fingerprint

Phodopus
Photoperiod
Neuropeptides
Weight Loss
Gene Expression
Weights and Measures
Food
Caloric Restriction
Pro-Opiomelanocortin
Neuropeptide Y
Brain-Derived Neurotrophic Factor
Amphetamine
Cocaine
Cricetinae
Genes
Leptin Receptors
Hyperphagia
Arcuate Nucleus of Hypothalamus
Peptide Receptors
Leptin

Keywords

  • animals
  • body weight
  • brain-derived neurotrophic factor
  • caloric restriction
  • cricetinae
  • eating
  • gene expression
  • hypothalamus
  • leptin
  • male
  • neuropeptides
  • organ size
  • phodopus
  • photoperiod
  • radioimmunoassay
  • weight loss

Cite this

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title = "Hypothalamic neuropeptide gene expression during recovery from food restriction superimposed on short-day photoperiod-induced weight loss in the Siberian hamster",
abstract = "Previously, 40{\%} food restriction of male Siberian hamsters over 21 days in short-day (SD) photoperiod induced characteristic changes in expression of hypothalamic arcuate nucleus energy balance genes; mRNAs for neuropeptide Y, agouti-related peptide, and leptin receptor were upregulated, and those of proopiomelanocortin and cocaine- and amphetamine-regulated transcript were depressed. The present study examined the effect of refeeding hamsters for 6 days (approximately 50{\%} recovery of weight differential) or 19 days (resumption of appropriate weight trajectory). Hyperphagia continued throughout refeeding, but differences in fat pad weights and leptin levels had disappeared after 19 days. Cocaine- and amphetamine-regulated transcript gene expression was depressed by prior restriction in both refed groups. The depressive effect of prior restriction on proopiomelanocortin gene expression had disappeared after 19 days of refeeding. There was no effect of prior food restriction on neuropeptide Y or agouti-related peptide gene expression. Expression of the anorexigenic brain-derived neurotrophic factor was downregulated in the ventromedial nucleus after SD exposure for 12 wk. In the SD food restriction study, there were effects of photoperiod on brain-derived neurotrophic factor gene expression but not of prior food restriction. Hypothalamic energy balance genes in the hamster respond asynchronously to return to a seasonally appropriate body weight. The achievement of this weight rather than the weight at which caloric restriction was imposed is the critical factor. The differential responses of hypothalamic energy balance genes to food restriction and refeeding are poorly characterized in any species, a critical issue given their potential relevance to human weight loss strategies that involve caloric restriction.",
keywords = "animals, body weight, brain-derived neurotrophic factor, caloric restriction, cricetinae, eating, gene expression, hypothalamus, leptin, male, neuropeptides, organ size, phodopus, photoperiod, radioimmunoassay, weight loss",
author = "Archer, {Zo{\"e} A.} and Kim-Marie Moar and Tracy Logie and Laura Reilly and Stevens, {Valerie Joan} and Morgan, {Peter John} and Julian Mercer",
year = "2007",
month = "9",
doi = "10.1152/ajpregu.00345.2007",
language = "English",
volume = "293",
pages = "R1094--R1101",
journal = "American Journal of Physiology-Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
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TY - JOUR

T1 - Hypothalamic neuropeptide gene expression during recovery from food restriction superimposed on short-day photoperiod-induced weight loss in the Siberian hamster

AU - Archer, Zoë A.

AU - Moar, Kim-Marie

AU - Logie, Tracy

AU - Reilly, Laura

AU - Stevens, Valerie Joan

AU - Morgan, Peter John

AU - Mercer, Julian

PY - 2007/9

Y1 - 2007/9

N2 - Previously, 40% food restriction of male Siberian hamsters over 21 days in short-day (SD) photoperiod induced characteristic changes in expression of hypothalamic arcuate nucleus energy balance genes; mRNAs for neuropeptide Y, agouti-related peptide, and leptin receptor were upregulated, and those of proopiomelanocortin and cocaine- and amphetamine-regulated transcript were depressed. The present study examined the effect of refeeding hamsters for 6 days (approximately 50% recovery of weight differential) or 19 days (resumption of appropriate weight trajectory). Hyperphagia continued throughout refeeding, but differences in fat pad weights and leptin levels had disappeared after 19 days. Cocaine- and amphetamine-regulated transcript gene expression was depressed by prior restriction in both refed groups. The depressive effect of prior restriction on proopiomelanocortin gene expression had disappeared after 19 days of refeeding. There was no effect of prior food restriction on neuropeptide Y or agouti-related peptide gene expression. Expression of the anorexigenic brain-derived neurotrophic factor was downregulated in the ventromedial nucleus after SD exposure for 12 wk. In the SD food restriction study, there were effects of photoperiod on brain-derived neurotrophic factor gene expression but not of prior food restriction. Hypothalamic energy balance genes in the hamster respond asynchronously to return to a seasonally appropriate body weight. The achievement of this weight rather than the weight at which caloric restriction was imposed is the critical factor. The differential responses of hypothalamic energy balance genes to food restriction and refeeding are poorly characterized in any species, a critical issue given their potential relevance to human weight loss strategies that involve caloric restriction.

AB - Previously, 40% food restriction of male Siberian hamsters over 21 days in short-day (SD) photoperiod induced characteristic changes in expression of hypothalamic arcuate nucleus energy balance genes; mRNAs for neuropeptide Y, agouti-related peptide, and leptin receptor were upregulated, and those of proopiomelanocortin and cocaine- and amphetamine-regulated transcript were depressed. The present study examined the effect of refeeding hamsters for 6 days (approximately 50% recovery of weight differential) or 19 days (resumption of appropriate weight trajectory). Hyperphagia continued throughout refeeding, but differences in fat pad weights and leptin levels had disappeared after 19 days. Cocaine- and amphetamine-regulated transcript gene expression was depressed by prior restriction in both refed groups. The depressive effect of prior restriction on proopiomelanocortin gene expression had disappeared after 19 days of refeeding. There was no effect of prior food restriction on neuropeptide Y or agouti-related peptide gene expression. Expression of the anorexigenic brain-derived neurotrophic factor was downregulated in the ventromedial nucleus after SD exposure for 12 wk. In the SD food restriction study, there were effects of photoperiod on brain-derived neurotrophic factor gene expression but not of prior food restriction. Hypothalamic energy balance genes in the hamster respond asynchronously to return to a seasonally appropriate body weight. The achievement of this weight rather than the weight at which caloric restriction was imposed is the critical factor. The differential responses of hypothalamic energy balance genes to food restriction and refeeding are poorly characterized in any species, a critical issue given their potential relevance to human weight loss strategies that involve caloric restriction.

KW - animals

KW - body weight

KW - brain-derived neurotrophic factor

KW - caloric restriction

KW - cricetinae

KW - eating

KW - gene expression

KW - hypothalamus

KW - leptin

KW - male

KW - neuropeptides

KW - organ size

KW - phodopus

KW - photoperiod

KW - radioimmunoassay

KW - weight loss

U2 - 10.1152/ajpregu.00345.2007

DO - 10.1152/ajpregu.00345.2007

M3 - Article

VL - 293

SP - R1094-R1101

JO - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 3

ER -