Identification and characterisation of 2-[125I]iodomelatonin binding and mella melatonin receptor expression in the human fetal leptomeninges

Janice Drew*, Lynda Williams, L T Hannah, Perry Barrett, David Abramovich, Peter John Morgan

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Melatonin binding sites were identified over the leptomeninges surrounding the human fetal brain using quantitative in vitro autoradiography and the melatonin agonist, 2-[125I]iodomelatonin. Binding was found to be saturable and of high affinity (dissociation constant (K(d))=54 pM and maximal theoretical binding (B(max)) = 13 fmol/mg protein), and inhibited by guanosine-5'-o-(3-thiotriphosphate) (GTPγS) suggesting that these binding sites represent G protein-coupled melatonin receptors. REIT-PCR performed on mRNA isolated from the human fetal leptomeninges detected expression of the G protein-coupled melatonin receptor Mella, but not Mellb. In situ hybridisation confirmed the localisation of Mella mRNA transcripts over the leptomeninges of the fetal brain. The identification of 2- [125I]iodomelatonin and Mella melatonin receptor expression in the fetal leptomeninges implies that melatonin may play a role in the early growth and development of the human brain.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalBrain Research
Volume761
Issue number1
DOIs
Publication statusPublished - 27 Jun 1997

Keywords

  • melatonin
  • melatonin receptor
  • leptomeninges
  • arachnoid
  • pia mater
  • human fetus
  • meningeal cells
  • sites
  • brain
  • rat
  • responses
  • neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Identification and characterisation of 2-[125I]iodomelatonin binding and mella melatonin receptor expression in the human fetal leptomeninges",
abstract = "Melatonin binding sites were identified over the leptomeninges surrounding the human fetal brain using quantitative in vitro autoradiography and the melatonin agonist, 2-[125I]iodomelatonin. Binding was found to be saturable and of high affinity (dissociation constant (K(d))=54 pM and maximal theoretical binding (B(max)) = 13 fmol/mg protein), and inhibited by guanosine-5'-o-(3-thiotriphosphate) (GTPγS) suggesting that these binding sites represent G protein-coupled melatonin receptors. REIT-PCR performed on mRNA isolated from the human fetal leptomeninges detected expression of the G protein-coupled melatonin receptor Mella, but not Mellb. In situ hybridisation confirmed the localisation of Mella mRNA transcripts over the leptomeninges of the fetal brain. The identification of 2- [125I]iodomelatonin and Mella melatonin receptor expression in the fetal leptomeninges implies that melatonin may play a role in the early growth and development of the human brain.",
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author = "Janice Drew and Lynda Williams and Hannah, {L T} and Perry Barrett and David Abramovich and Morgan, {Peter John}",
year = "1997",
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T1 - Identification and characterisation of 2-[125I]iodomelatonin binding and mella melatonin receptor expression in the human fetal leptomeninges

AU - Drew, Janice

AU - Williams, Lynda

AU - Hannah, L T

AU - Barrett, Perry

AU - Abramovich, David

AU - Morgan, Peter John

PY - 1997/6/27

Y1 - 1997/6/27

N2 - Melatonin binding sites were identified over the leptomeninges surrounding the human fetal brain using quantitative in vitro autoradiography and the melatonin agonist, 2-[125I]iodomelatonin. Binding was found to be saturable and of high affinity (dissociation constant (K(d))=54 pM and maximal theoretical binding (B(max)) = 13 fmol/mg protein), and inhibited by guanosine-5'-o-(3-thiotriphosphate) (GTPγS) suggesting that these binding sites represent G protein-coupled melatonin receptors. REIT-PCR performed on mRNA isolated from the human fetal leptomeninges detected expression of the G protein-coupled melatonin receptor Mella, but not Mellb. In situ hybridisation confirmed the localisation of Mella mRNA transcripts over the leptomeninges of the fetal brain. The identification of 2- [125I]iodomelatonin and Mella melatonin receptor expression in the fetal leptomeninges implies that melatonin may play a role in the early growth and development of the human brain.

AB - Melatonin binding sites were identified over the leptomeninges surrounding the human fetal brain using quantitative in vitro autoradiography and the melatonin agonist, 2-[125I]iodomelatonin. Binding was found to be saturable and of high affinity (dissociation constant (K(d))=54 pM and maximal theoretical binding (B(max)) = 13 fmol/mg protein), and inhibited by guanosine-5'-o-(3-thiotriphosphate) (GTPγS) suggesting that these binding sites represent G protein-coupled melatonin receptors. REIT-PCR performed on mRNA isolated from the human fetal leptomeninges detected expression of the G protein-coupled melatonin receptor Mella, but not Mellb. In situ hybridisation confirmed the localisation of Mella mRNA transcripts over the leptomeninges of the fetal brain. The identification of 2- [125I]iodomelatonin and Mella melatonin receptor expression in the fetal leptomeninges implies that melatonin may play a role in the early growth and development of the human brain.

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KW - rat

KW - responses

KW - neurons

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