Abstract
By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.
| Original language | English |
|---|---|
| Pages (from-to) | 614-20 |
| Number of pages | 7 |
| Journal | Developmental Dynamics |
| Volume | 231 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 2004 |
Bibliographical note
(c) 2004 Wiley-Liss, Inc.Keywords
- Animals
- Body Patterning
- DNA, Complementary
- Embryonic Development
- Eye Proteins
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Genome
- Homeodomain Proteins
- In Situ Hybridization
- Mice
- Mice, Knockout
- Mutation
- Oligonucleotide Array Sequence Analysis
- Paired Box Transcription Factors
- Repressor Proteins
- Reverse Transcriptase Polymerase Chain Reaction
- Telencephalon
- Time Factors
- Transcription Factors