Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

Andrea Faedo, Jane C Quinn, Patrick Niall Stoney, Jason E Long, Catherine Dye, Massimo Zollo, John L R Rubenstein, David J Price, Alessandro Bulfone

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

Original languageEnglish
Pages (from-to)614-20
Number of pages7
JournalDevelopmental Dynamics
Volume231
Issue number3
DOIs
Publication statusPublished - Nov 2004

    Fingerprint

Keywords

  • Animals
  • Body Patterning
  • DNA, Complementary
  • Embryonic Development
  • Eye Proteins
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genome
  • Homeodomain Proteins
  • In Situ Hybridization
  • Mice
  • Mice, Knockout
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Paired Box Transcription Factors
  • Repressor Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Telencephalon
  • Time Factors
  • Transcription Factors

Cite this

Faedo, A., Quinn, J. C., Stoney, P. N., Long, J. E., Dye, C., Zollo, M., Rubenstein, J. L. R., Price, D. J., & Bulfone, A. (2004). Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon. Developmental Dynamics, 231(3), 614-20. https://doi.org/10.1002/dvdy.20152