Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

Andrea Faedo, Jane C Quinn, Patrick Niall Stoney, Jason E Long, Catherine Dye, Massimo Zollo, John L R Rubenstein, David J Price, Alessandro Bulfone

Research output: Contribution to journalArticle

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Abstract

By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

Original languageEnglish
Pages (from-to)614-20
Number of pages7
JournalDevelopmental Dynamics
Volume231
Issue number3
DOIs
Publication statusPublished - Nov 2004

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Telencephalon
Median Eminence
Prosencephalon
Brain
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Thalamus
Transcriptome
Hypothalamus
In Situ Hybridization
RNA

Keywords

  • Animals
  • Body Patterning
  • DNA, Complementary
  • Embryonic Development
  • Eye Proteins
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genome
  • Homeodomain Proteins
  • In Situ Hybridization
  • Mice
  • Mice, Knockout
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Paired Box Transcription Factors
  • Repressor Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Telencephalon
  • Time Factors
  • Transcription Factors

Cite this

Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon. / Faedo, Andrea; Quinn, Jane C; Stoney, Patrick Niall; Long, Jason E; Dye, Catherine; Zollo, Massimo; Rubenstein, John L R; Price, David J; Bulfone, Alessandro.

In: Developmental Dynamics, Vol. 231, No. 3, 11.2004, p. 614-20.

Research output: Contribution to journalArticle

Faedo, A, Quinn, JC, Stoney, PN, Long, JE, Dye, C, Zollo, M, Rubenstein, JLR, Price, DJ & Bulfone, A 2004, 'Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon', Developmental Dynamics, vol. 231, no. 3, pp. 614-20. https://doi.org/10.1002/dvdy.20152
Faedo, Andrea ; Quinn, Jane C ; Stoney, Patrick Niall ; Long, Jason E ; Dye, Catherine ; Zollo, Massimo ; Rubenstein, John L R ; Price, David J ; Bulfone, Alessandro. / Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon. In: Developmental Dynamics. 2004 ; Vol. 231, No. 3. pp. 614-20.
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AU - Faedo, Andrea

AU - Quinn, Jane C

AU - Stoney, Patrick Niall

AU - Long, Jason E

AU - Dye, Catherine

AU - Zollo, Massimo

AU - Rubenstein, John L R

AU - Price, David J

AU - Bulfone, Alessandro

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AB - By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

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KW - Body Patterning

KW - DNA, Complementary

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KW - Eye Proteins

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Genome

KW - Homeodomain Proteins

KW - In Situ Hybridization

KW - Mice

KW - Mice, Knockout

KW - Mutation

KW - Oligonucleotide Array Sequence Analysis

KW - Paired Box Transcription Factors

KW - Repressor Proteins

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Telencephalon

KW - Time Factors

KW - Transcription Factors

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DO - 10.1002/dvdy.20152

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VL - 231

SP - 614

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JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 3

ER -