Identification and expression analysis of an atypical chemokine receptor-2 (ACKR2)/CC chemokine binding protein-2 (CCBP2) in rainbow trout (Oncorhynchus mykiss)

Zhitao Qi, Yousheng Jiang, Jason W Holland, Pin Nie, Christopher J Secombes, Tiehui Wang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Atypical chemokine receptors (ACKRs) have emerged as key components of the chemokine system, with an essential regulatory function in innate and adaptive immune responses and inflammation. In mammals ACKR2 is a 'scavenging' receptor for inflammatory CC chemokines and plays a central role in the resolution of in vivo inflammatory responses. An ACKR2 like gene has been identified and cloned in rainbow trout (Teleostei) in the present study, enabling the further identification of this molecule in another group of ray-finned teleost fish (Holostei), in a lobe-finned fish (Sarcopterygii-coelacanth), and in reptiles. The identity of these ACKR2 molecules is supported by their conserved structure, and by phylogenetic tree and synteny analysis. Trout ACKR2 is highly expressed in spleen and head kidney, suggesting a homeostatic role of this receptor in limiting the availability of its potential ligands. Trout ACKR2 expression can be modulated in vivo by bacterial and parasitic infections, and in vitro by PAMPs (poly I:C and peptidoglycan) and cytokines (IL-6, TNF-α, IFN-γ and IL-21) in a time dependent manner. These patterns of expression and modulation suggest that trout ACKR2 is regulated in a complex way and has an important role in control of the chemokine network in fish as in mammals.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalFish & Shellfish Immunology
Volume44
Issue number2
Early online date3 Mar 2015
DOIs
Publication statusPublished - Jun 2015

Fingerprint

CC Chemokines
Chemokine Receptors
rainbow
binding proteins
Oncorhynchus mykiss
protein
mammal
fish
Fish
trout
Mammals
immune response
teleost
reptile
ligand
chemokines
Chemokines
phylogenetics
inflammation
mammals

Keywords

  • Rainbow trout Oncorhynchus mykiss
  • Atypical chemokine receptor-2 (ACKR2)
  • CC chemokine binding protein-2 (CCBP2)
  • expression
  • infection

Cite this

@article{93f11ab879f149009df811d65948c955,
title = "Identification and expression analysis of an atypical chemokine receptor-2 (ACKR2)/CC chemokine binding protein-2 (CCBP2) in rainbow trout (Oncorhynchus mykiss)",
abstract = "Atypical chemokine receptors (ACKRs) have emerged as key components of the chemokine system, with an essential regulatory function in innate and adaptive immune responses and inflammation. In mammals ACKR2 is a 'scavenging' receptor for inflammatory CC chemokines and plays a central role in the resolution of in vivo inflammatory responses. An ACKR2 like gene has been identified and cloned in rainbow trout (Teleostei) in the present study, enabling the further identification of this molecule in another group of ray-finned teleost fish (Holostei), in a lobe-finned fish (Sarcopterygii-coelacanth), and in reptiles. The identity of these ACKR2 molecules is supported by their conserved structure, and by phylogenetic tree and synteny analysis. Trout ACKR2 is highly expressed in spleen and head kidney, suggesting a homeostatic role of this receptor in limiting the availability of its potential ligands. Trout ACKR2 expression can be modulated in vivo by bacterial and parasitic infections, and in vitro by PAMPs (poly I:C and peptidoglycan) and cytokines (IL-6, TNF-α, IFN-γ and IL-21) in a time dependent manner. These patterns of expression and modulation suggest that trout ACKR2 is regulated in a complex way and has an important role in control of the chemokine network in fish as in mammals.",
keywords = "Rainbow trout Oncorhynchus mykiss, Atypical chemokine receptor-2 (ACKR2), CC chemokine binding protein-2 (CCBP2), expression, infection",
author = "Zhitao Qi and Yousheng Jiang and Holland, {Jason W} and Pin Nie and Secombes, {Christopher J} and Tiehui Wang",
note = "Copyright {\circledC} 2015 Elsevier Ltd. All rights reserved. This work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. ZQ was supported by the Overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. YJ was supported financially by the National Scholarship Council of Shanghai City, China and JWH by the Biotechnology and Biological Sciences Research Council (BB/K009125/1).",
year = "2015",
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T1 - Identification and expression analysis of an atypical chemokine receptor-2 (ACKR2)/CC chemokine binding protein-2 (CCBP2) in rainbow trout (Oncorhynchus mykiss)

AU - Qi, Zhitao

AU - Jiang, Yousheng

AU - Holland, Jason W

AU - Nie, Pin

AU - Secombes, Christopher J

AU - Wang, Tiehui

N1 - Copyright © 2015 Elsevier Ltd. All rights reserved. This work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. ZQ was supported by the Overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. YJ was supported financially by the National Scholarship Council of Shanghai City, China and JWH by the Biotechnology and Biological Sciences Research Council (BB/K009125/1).

PY - 2015/6

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N2 - Atypical chemokine receptors (ACKRs) have emerged as key components of the chemokine system, with an essential regulatory function in innate and adaptive immune responses and inflammation. In mammals ACKR2 is a 'scavenging' receptor for inflammatory CC chemokines and plays a central role in the resolution of in vivo inflammatory responses. An ACKR2 like gene has been identified and cloned in rainbow trout (Teleostei) in the present study, enabling the further identification of this molecule in another group of ray-finned teleost fish (Holostei), in a lobe-finned fish (Sarcopterygii-coelacanth), and in reptiles. The identity of these ACKR2 molecules is supported by their conserved structure, and by phylogenetic tree and synteny analysis. Trout ACKR2 is highly expressed in spleen and head kidney, suggesting a homeostatic role of this receptor in limiting the availability of its potential ligands. Trout ACKR2 expression can be modulated in vivo by bacterial and parasitic infections, and in vitro by PAMPs (poly I:C and peptidoglycan) and cytokines (IL-6, TNF-α, IFN-γ and IL-21) in a time dependent manner. These patterns of expression and modulation suggest that trout ACKR2 is regulated in a complex way and has an important role in control of the chemokine network in fish as in mammals.

AB - Atypical chemokine receptors (ACKRs) have emerged as key components of the chemokine system, with an essential regulatory function in innate and adaptive immune responses and inflammation. In mammals ACKR2 is a 'scavenging' receptor for inflammatory CC chemokines and plays a central role in the resolution of in vivo inflammatory responses. An ACKR2 like gene has been identified and cloned in rainbow trout (Teleostei) in the present study, enabling the further identification of this molecule in another group of ray-finned teleost fish (Holostei), in a lobe-finned fish (Sarcopterygii-coelacanth), and in reptiles. The identity of these ACKR2 molecules is supported by their conserved structure, and by phylogenetic tree and synteny analysis. Trout ACKR2 is highly expressed in spleen and head kidney, suggesting a homeostatic role of this receptor in limiting the availability of its potential ligands. Trout ACKR2 expression can be modulated in vivo by bacterial and parasitic infections, and in vitro by PAMPs (poly I:C and peptidoglycan) and cytokines (IL-6, TNF-α, IFN-γ and IL-21) in a time dependent manner. These patterns of expression and modulation suggest that trout ACKR2 is regulated in a complex way and has an important role in control of the chemokine network in fish as in mammals.

KW - Rainbow trout Oncorhynchus mykiss

KW - Atypical chemokine receptor-2 (ACKR2)

KW - CC chemokine binding protein-2 (CCBP2)

KW - expression

KW - infection

U2 - 10.1016/j.fsi.2015.02.038

DO - 10.1016/j.fsi.2015.02.038

M3 - Article

VL - 44

SP - 389

EP - 398

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

IS - 2

ER -