Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens

J Machell, A H M Van den Broek, A J Nisbet, H R P Miller, R E Isaac, J F Huntley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Infestation of sheep with the ectoparasitic mite Psoroptes ovis, results in a severe allergic dermatitis. Currently, little is known about the allergens/antigens that stimulate the allergic response. We have isolated an 836-bp cDNA from a P. ovis cDNA library which displays strong homology to cysteine proteases and, in particular, to the group I house dust mite allergens Der p 1, Der f 1 and Eur m 1. The cDNA was expressed in Escherchia coli, fused to a hexahistidine tag and the recombinant protein (Pso o 1) purified using a nickel-affinity column. The recombinant Pso o 1 was tested for recognition by immunoglobulin (Ig) G and IgE in serum from P. ovis naive and P. ovis infested sheep. Using Western blots, both classes of antibody to Pso o 1 were detected in postinfestation serum. In enzyme-linked immunosorbent assays, a pronounced IgG-antibody response to Pso o 1 was detected in five of five sheep 3 weeks postinfestation. The IgE-antibody response to whole mite extract was poor in four of five animals. However, a marked IgE response occurred in the fifth animal, and IgE anti Pso o 1 was detected in the serum.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalParasite Immunology
Volume24
Publication statusPublished - 2002

Keywords

  • Psoroptes ovis
  • sheep-arthropoda
  • sheep scab
  • cysteine protease
  • allergen
  • antigen
  • P-I
  • MOLECULAR CHARACTERIZATION
  • MAJOR ALLERGEN
  • IGE ANTIBODIES
  • BINDING
  • DERMATOPHAGOIDES
  • PTERONYSSINUS
  • PURIFICATION
  • EXPRESSION
  • PROTEINS

Cite this

Machell, J., Van den Broek, A. H. M., Nisbet, A. J., Miller, H. R. P., Isaac, R. E., & Huntley, J. F. (2002). Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens. Parasite Immunology, 24, 413-422.

Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens. / Machell, J ; Van den Broek, A H M ; Nisbet, A J ; Miller, H R P ; Isaac, R E ; Huntley, J F .

In: Parasite Immunology, Vol. 24, 2002, p. 413-422.

Research output: Contribution to journalArticle

Machell, J, Van den Broek, AHM, Nisbet, AJ, Miller, HRP, Isaac, RE & Huntley, JF 2002, 'Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens', Parasite Immunology, vol. 24, pp. 413-422.
Machell J, Van den Broek AHM, Nisbet AJ, Miller HRP, Isaac RE, Huntley JF. Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens. Parasite Immunology. 2002;24:413-422.
Machell, J ; Van den Broek, A H M ; Nisbet, A J ; Miller, H R P ; Isaac, R E ; Huntley, J F . / Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens. In: Parasite Immunology. 2002 ; Vol. 24. pp. 413-422.
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