Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens

J Machell, A H M Van den Broek, A J Nisbet, H R P Miller, R E Isaac, J F Huntley

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Abstract

Infestation of sheep with the ectoparasitic mite Psoroptes ovis, results in a severe allergic dermatitis. Currently, little is known about the allergens/antigens that stimulate the allergic response. We have isolated an 836-bp cDNA from a P. ovis cDNA library which displays strong homology to cysteine proteases and, in particular, to the group I house dust mite allergens Der p 1, Der f 1 and Eur m 1. The cDNA was expressed in Escherchia coli, fused to a hexahistidine tag and the recombinant protein (Pso o 1) purified using a nickel-affinity column. The recombinant Pso o 1 was tested for recognition by immunoglobulin (Ig) G and IgE in serum from P. ovis naive and P. ovis infested sheep. Using Western blots, both classes of antibody to Pso o 1 were detected in postinfestation serum. In enzyme-linked immunosorbent assays, a pronounced IgG-antibody response to Pso o 1 was detected in five of five sheep 3 weeks postinfestation. The IgE-antibody response to whole mite extract was poor in four of five animals. However, a marked IgE response occurred in the fifth animal, and IgE anti Pso o 1 was detected in the serum.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalParasite Immunology
Volume24
Publication statusPublished - 2002

Keywords

  • Psoroptes ovis
  • sheep-arthropoda
  • sheep scab
  • cysteine protease
  • allergen
  • antigen
  • P-I
  • MOLECULAR CHARACTERIZATION
  • MAJOR ALLERGEN
  • IGE ANTIBODIES
  • BINDING
  • DERMATOPHAGOIDES
  • PTERONYSSINUS
  • PURIFICATION
  • EXPRESSION
  • PROTEINS

Cite this

Machell, J., Van den Broek, A. H. M., Nisbet, A. J., Miller, H. R. P., Isaac, R. E., & Huntley, J. F. (2002). Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens. Parasite Immunology, 24, 413-422.