Abstract
Infestation of sheep with the ectoparasitic mite Psoroptes ovis, results in a severe allergic dermatitis. Currently, little is known about the allergens/antigens that stimulate the allergic response. We have isolated an 836-bp cDNA from a P. ovis cDNA library which displays strong homology to cysteine proteases and, in particular, to the group I house dust mite allergens Der p 1, Der f 1 and Eur m 1. The cDNA was expressed in Escherchia coli, fused to a hexahistidine tag and the recombinant protein (Pso o 1) purified using a nickel-affinity column. The recombinant Pso o 1 was tested for recognition by immunoglobulin (Ig) G and IgE in serum from P. ovis naive and P. ovis infested sheep. Using Western blots, both classes of antibody to Pso o 1 were detected in postinfestation serum. In enzyme-linked immunosorbent assays, a pronounced IgG-antibody response to Pso o 1 was detected in five of five sheep 3 weeks postinfestation. The IgE-antibody response to whole mite extract was poor in four of five animals. However, a marked IgE response occurred in the fifth animal, and IgE anti Pso o 1 was detected in the serum.
Original language | English |
---|---|
Pages (from-to) | 413-422 |
Number of pages | 10 |
Journal | Parasite Immunology |
Volume | 24 |
Publication status | Published - 2002 |
Keywords
- Psoroptes ovis
- sheep-arthropoda
- sheep scab
- cysteine protease
- allergen
- antigen
- P-I
- MOLECULAR CHARACTERIZATION
- MAJOR ALLERGEN
- IGE ANTIBODIES
- BINDING
- DERMATOPHAGOIDES
- PTERONYSSINUS
- PURIFICATION
- EXPRESSION
- PROTEINS