Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically Informative Nucleotide Sequencing

M. J. Chapela; C. G. Sotelo; R. I. Perez-Martin; P. Calo-Mata; H. Rehbein; Georgina Louise Hold; J. Quinterio; M. Rey-Mendez; C. Rosa; A. Santos

doi:10.1111/j.1365-2621.2002.tb08703.x

Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically Informative Nucleotide Sequencing

M. J. Chapela, C. G. Sotelo, R. I. Perez-Martin, P. Calo-Mata, H. Rehbein, Georgina Louise Hold, J. Quinterio, M. Rey-Mendez, C. Rosa, A. Santos

Applied Medicine

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

An identification technique of commercially important cephalopods based on 16s RNA analysis was developed. A set of primers was designed to amplify a fragment of approximately 200 bp that presents enough variability for reliable species identification. Sequences from this fragment of 9 different authentic species were studied, genetic distances were measured, and a phylogenetic tree was constructed, with individuals of the same species grouped within the same cluster. Eight different types of commercial seafood products, mainly labelled as “squid rings”, were analyzed and sequences were employed for species identification showing that FINS is a suitable technique for identification of processed cephalopods.

Original language	English
Pages (from-to)	1672-1676
Number of pages	4
Journal	Journal of Food Science
Volume	67
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2621.2002.tb08703.x
Publication status	Published - Jun 2002

Keywords

seafood
PCR
species identification
cephalopods
16s RNA gene
FINS

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10.1111/j.1365-2621.2002.tb08703.x

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Chapela, M. J., Sotelo, C. G., Perez-Martin, R. I., Calo-Mata, P., Rehbein, H., Hold, G. L., Quinterio, J., Rey-Mendez, M., Rosa, C., & Santos, A. (2002). Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically Informative Nucleotide Sequencing. Journal of Food Science, 67(5), 1672-1676. https://doi.org/10.1111/j.1365-2621.2002.tb08703.x

Chapela, MJ, Sotelo, CG, Perez-Martin, RI, Calo-Mata, P, Rehbein, H, Hold, GL, Quinterio, J, Rey-Mendez, M, Rosa, C & Santos, A 2002, 'Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically Informative Nucleotide Sequencing', Journal of Food Science, vol. 67, no. 5, pp. 1672-1676. https://doi.org/10.1111/j.1365-2621.2002.tb08703.x

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title = "Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically Informative Nucleotide Sequencing",

abstract = "An identification technique of commercially important cephalopods based on 16s RNA analysis was developed. A set of primers was designed to amplify a fragment of approximately 200 bp that presents enough variability for reliable species identification. Sequences from this fragment of 9 different authentic species were studied, genetic distances were measured, and a phylogenetic tree was constructed, with individuals of the same species grouped within the same cluster. Eight different types of commercial seafood products, mainly labelled as “squid rings”, were analyzed and sequences were employed for species identification showing that FINS is a suitable technique for identification of processed cephalopods. ",

keywords = "seafood, PCR, species identification, cephalopods, 16s RNA gene, FINS",

author = "Chapela, {M. J.} and Sotelo, {C. G.} and Perez-Martin, {R. I.} and P. Calo-Mata and H. Rehbein and Hold, {Georgina Louise} and J. Quinterio and M. Rey-Mendez and C. Rosa and A. Santos",

year = "2002",

month = jun,

doi = "10.1111/j.1365-2621.2002.tb08703.x",

language = "English",

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AU - Chapela, M. J.

AU - Sotelo, C. G.

AU - Perez-Martin, R. I.

AU - Calo-Mata, P.

AU - Rehbein, H.

AU - Hold, Georgina Louise

AU - Quinterio, J.

AU - Rey-Mendez, M.

AU - Rosa, C.

AU - Santos, A.

PY - 2002/6

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KW - seafood

KW - PCR

KW - species identification

KW - cephalopods

KW - 16s RNA gene

KW - FINS

U2 - 10.1111/j.1365-2621.2002.tb08703.x

DO - 10.1111/j.1365-2621.2002.tb08703.x

M3 - Article

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VL - 67

SP - 1672

EP - 1676

JO - Journal of Food Science

JF - Journal of Food Science

IS - 5

ER -

Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically Informative Nucleotide Sequencing

Abstract

Keywords

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