Identification of interleukin-22 in gadoids and examination of its expression level in vaccinated fish

Yolanda Corripio Miyar, Jun Zou, Heather Richmond, Christopher John Secombes

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

This paper reports the cloning and sequencing of interleukin (IL)-22 in two gadoid fish, cod (Gadus morhua) and haddock (Melanogrammus aeglefinus). The complete transcript of this gene was 1002 and 1154bp respectively, of which 492 bp was the open reading frame (ORF) in both genes. High amino acid identity (88.3%) was found between these genes but was less than 50% aa identity to other known genes. The gene organisation of haddock IL-22 consisted of five exons and four introns, as with other IL-10 family members. Expression studies showed that IL-22 is constitutively expressed in gill, with low level expression also observed in gut, gonad and head kidney In a vaccination experiment, haddock were injected intraperitoneally with formalin-killed Vibrio anguillarum or with PBS, and 2 months later challenged by immersion in 10(7) cfu/ml V. anguillarum for 30 min. Head kidney and gill samples were collected prior to challenge and 24, 48 and 72 h post-challenge (hpc) for Real-time PCR analysis of IL-22 expression. No significant changes in IL-22 expression were observed in head kidney tissue but vaccinated fish showed a significantly increased expression of IL-22 24hpc in gill and no mortalities were seen in these fish. In contrast, control fish, which started to succumb to the disease from 72hpc, showed no significant increase in gill expression after challenge. (C) 2009 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)2098-2106
Number of pages9
JournalMolecular Immunology
Volume46
Issue number10
Early online date28 Apr 2009
DOIs
Publication statusPublished - Jun 2009

Keywords

  • Haddock
  • Cod
  • TH17 cytokine
  • Interleukin-22
  • Vaccine efficacy
  • Teleost fish
  • Gadus-Morhua L.
  • T-cell
  • Melanogrammus-Aeglefinus
  • Vibrio-Anguillarum
  • Sequence alignment
  • TH17 cytokines
  • Host-Defense
  • Atlantic Cod
  • Inflammation
  • Responses

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