Identification of plasmids in the genus Marinococcus and complete nucleotide sequence of plasmid pPL1 from Marinococcus halophilus

P Louis, E A Galinski

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Several plasmids were detected in the Gram-positive halophilic eubacterium Marinococcus halophilus and in the related strain M52. The complete nucleotide sequence (3874 bp) of one of these plasmids, pPL1, was determined. Four major open reading frames were identified. Whereas orf3 and orf4 showed no sequence similarities to known proteins, rep displayed a high sequence similarity to replication proteins of rolling circle plasmids. Upstream of this ORE a sequence resembling the double-strand origin was detected. A region probably constituting the single-strand origin was identified. The ORF mob showed sequence similarity with Mob proteins of rolling circle plasmids. The observed characteristics suggest that pPL1 replicates according to the rolling circle mechanism. (C) 1998 Academic Press.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalPlasmid
Volume38
Issue number2
Publication statusPublished - 1997

Keywords

  • HOST-RANGE PLASMID
  • ESCHERICHIA-COLI
  • STRUCTURAL ORGANIZATION
  • DEOXYRIBONUCLEIC-ACID
  • COMPATIBLE SOLUTES
  • CRYPTIC PLASMID
  • COMB-NOV
  • DNA
  • EUBACTERIA
  • BACTERIA

Cite this

Identification of plasmids in the genus Marinococcus and complete nucleotide sequence of plasmid pPL1 from Marinococcus halophilus. / Louis, P ; Galinski, E A .

In: Plasmid, Vol. 38, No. 2, 1997, p. 107-114.

Research output: Contribution to journalArticle

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T1 - Identification of plasmids in the genus Marinococcus and complete nucleotide sequence of plasmid pPL1 from Marinococcus halophilus

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AU - Galinski, E A

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AB - Several plasmids were detected in the Gram-positive halophilic eubacterium Marinococcus halophilus and in the related strain M52. The complete nucleotide sequence (3874 bp) of one of these plasmids, pPL1, was determined. Four major open reading frames were identified. Whereas orf3 and orf4 showed no sequence similarities to known proteins, rep displayed a high sequence similarity to replication proteins of rolling circle plasmids. Upstream of this ORE a sequence resembling the double-strand origin was detected. A region probably constituting the single-strand origin was identified. The ORF mob showed sequence similarity with Mob proteins of rolling circle plasmids. The observed characteristics suggest that pPL1 replicates according to the rolling circle mechanism. (C) 1998 Academic Press.

KW - HOST-RANGE PLASMID

KW - ESCHERICHIA-COLI

KW - STRUCTURAL ORGANIZATION

KW - DEOXYRIBONUCLEIC-ACID

KW - COMPATIBLE SOLUTES

KW - CRYPTIC PLASMID

KW - COMB-NOV

KW - DNA

KW - EUBACTERIA

KW - BACTERIA

M3 - Article

VL - 38

SP - 107

EP - 114

JO - Plasmid

JF - Plasmid

SN - 0147-619X

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