Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Andrew Aitken, P Cohen, S Santikarn, Dudley H Williams, Alexander Graham Calder, Alistair Smith, C B Klee

    Research output: Contribution to journalArticle

    167 Citations (Scopus)

    Abstract

    The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly-Asn-Glu-Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.
    Original languageEnglish
    Pages (from-to)314-318
    Number of pages5
    JournalFEBS Letters
    Volume150
    Issue number2
    DOIs
    Publication statusPublished - 27 Dec 1982

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    Calcineurin
    Myristic Acid
    Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
    Fast Atom Bombardment Mass Spectrometry
    Gas chromatography
    Gas Chromatography-Mass Spectrometry
    Mass spectrometry
    Proteins
    Fatty Acids
    High Pressure Liquid Chromatography
    Atoms
    Peptides
    calcineurin phosphatase
    acetonitrile

    Keywords

    • Amino Acids
    • Animals
    • Brain Chemistry
    • Calcium-Binding Proteins
    • Calmodulin-Binding Proteins
    • Cattle
    • Chromatography, Gas
    • Mass Spectrometry
    • Myristic Acid
    • Myristic Acids
    • Nerve Tissue Proteins
    • Protein phosphatase
    • Ca2+
    • Calmodulin
    • Mass spectrometry
    • Fatty acids
    • High-performance liquid High-performance liquid chromatography

    Cite this

    Aitken, A., Cohen, P., Santikarn, S., Williams, D. H., Calder, A. G., Smith, A., & Klee, C. B. (1982). Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. FEBS Letters, 150(2), 314-318. https://doi.org/10.1016/0014-5793(82)80759-X

    Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. / Aitken, Andrew; Cohen, P; Santikarn, S; Williams, Dudley H; Calder, Alexander Graham; Smith, Alistair; Klee, C B.

    In: FEBS Letters, Vol. 150, No. 2, 27.12.1982, p. 314-318.

    Research output: Contribution to journalArticle

    Aitken, A, Cohen, P, Santikarn, S, Williams, DH, Calder, AG, Smith, A & Klee, CB 1982, 'Identification of the NH2-terminal blocking group of calcineurin B as myristic acid', FEBS Letters, vol. 150, no. 2, pp. 314-318. https://doi.org/10.1016/0014-5793(82)80759-X
    Aitken A, Cohen P, Santikarn S, Williams DH, Calder AG, Smith A et al. Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. FEBS Letters. 1982 Dec 27;150(2):314-318. https://doi.org/10.1016/0014-5793(82)80759-X
    Aitken, Andrew ; Cohen, P ; Santikarn, S ; Williams, Dudley H ; Calder, Alexander Graham ; Smith, Alistair ; Klee, C B. / Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. In: FEBS Letters. 1982 ; Vol. 150, No. 2. pp. 314-318.
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    AU - Smith, Alistair

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