Exophiala angulospora infection in hatchery reared lumpfish (Cyclopterus lumpus) broodstock

Marcia Saraiva, Max J Beckmann, Sara Pflaum, Marianne Pearson , Daniel Carcajona, James W Treasurer, Pieter Van West (Corresponding Author)

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Abstract

Samples from moribund lumpfish were collected in a marine hatchery in Scotland in 2015. Black nodules were noted on the skin, and gills and fungal hyphae were extensively distributed in musculature and internal organs. Multifocal chronic inflammatory lesions displaced structures in all affected organs. Mortalities commenced on completion of spawning in May and were evenly distributed over the second year in the temperature range 11–15°C. The main systemic infection causing agent was initially identified based on morphological characteristics as an Exophiala species. Ribosomal DNA (rDNA) ITS regions of the isolates were subsequently sequenced confirming the isolates belonged to Exophiala genus. All isolates fell in a single phylogenetic cluster, which is represented by Exophiala angulospora. Fish were treated with either formalin or Bronopol or a combination of both, but there was no effect on the pattern or numbers of mortalities. Isolates were also tested against three different concentrations of Latrunculin A, Amphotericin B and Itraconazole with no success. It is of utmost importance to increase the knowledge on pathogen–host interactions to successfully develop sustainable control methods.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalJournal of Fish Diseases
Volume42
Issue number3
Early online date11 Jan 2019
DOIs
Publication statusPublished - Mar 2019

Fingerprint

Cyclopterus
Cylopterus lumpus
Exophiala
breeding stock
hatchery
hatcheries
mortality
Infection
lesion
infection
skin
spawning
Itraconazole
itraconazole
Hyphae
Mortality
amphotericin B
Scotland
Amphotericin B
Ribosomal DNA

Keywords

  • cleaner fish
  • Cyclopterus lumpus
  • Exophiala angulospora
  • fungus
  • lumpfish
  • Fungus
  • Anti-Infective Agents/pharmacology
  • DNA, Ribosomal
  • Scotland
  • Phylogeny
  • Phaeohyphomycosis/drug therapy
  • Exophiala/classification
  • Fish Diseases/drug therapy
  • Animals
  • Antifungal Agents/pharmacology
  • Perciformes
  • Aquaculture
  • GENETICS
  • DISEASE

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Cite this

Saraiva, M., Beckmann, M. J., Pflaum, S., Pearson , M., Carcajona, D., Treasurer, J. W., & Van West, P. (2019). Exophiala angulospora infection in hatchery reared lumpfish (Cyclopterus lumpus) broodstock. Journal of Fish Diseases, 42(3), 335-343. https://doi.org/10.1111/jfd.12940

Exophiala angulospora infection in hatchery reared lumpfish (Cyclopterus lumpus) broodstock. / Saraiva, Marcia; Beckmann, Max J; Pflaum, Sara; Pearson , Marianne; Carcajona, Daniel; Treasurer, James W; Van West, Pieter (Corresponding Author).

In: Journal of Fish Diseases, Vol. 42, No. 3, 03.2019, p. 335-343.

Research output: Contribution to journalArticle

Saraiva, M, Beckmann, MJ, Pflaum, S, Pearson , M, Carcajona, D, Treasurer, JW & Van West, P 2019, 'Exophiala angulospora infection in hatchery reared lumpfish (Cyclopterus lumpus) broodstock', Journal of Fish Diseases, vol. 42, no. 3, pp. 335-343. https://doi.org/10.1111/jfd.12940
Saraiva M, Beckmann MJ, Pflaum S, Pearson M, Carcajona D, Treasurer JW et al. Exophiala angulospora infection in hatchery reared lumpfish (Cyclopterus lumpus) broodstock. Journal of Fish Diseases. 2019 Mar;42(3):335-343. https://doi.org/10.1111/jfd.12940
Saraiva, Marcia ; Beckmann, Max J ; Pflaum, Sara ; Pearson , Marianne ; Carcajona, Daniel ; Treasurer, James W ; Van West, Pieter. / Exophiala angulospora infection in hatchery reared lumpfish (Cyclopterus lumpus) broodstock. In: Journal of Fish Diseases. 2019 ; Vol. 42, No. 3. pp. 335-343.
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abstract = "Samples from moribund lumpfish were collected in a marine hatchery in Scotland in 2015. Black nodules were noted on the skin, and gills and fungal hyphae were extensively distributed in musculature and internal organs. Multifocal chronic inflammatory lesions displaced structures in all affected organs. Mortalities commenced on completion of spawning in May and were evenly distributed over the second year in the temperature range 11–15°C. The main systemic infection causing agent was initially identified based on morphological characteristics as an Exophiala species. Ribosomal DNA (rDNA) ITS regions of the isolates were subsequently sequenced confirming the isolates belonged to Exophiala genus. All isolates fell in a single phylogenetic cluster, which is represented by Exophiala angulospora. Fish were treated with either formalin or Bronopol or a combination of both, but there was no effect on the pattern or numbers of mortalities. Isolates were also tested against three different concentrations of Latrunculin A, Amphotericin B and Itraconazole with no success. It is of utmost importance to increase the knowledge on pathogen–host interactions to successfully develop sustainable control methods.",
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