Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.): Evidence for intramolecular-induced suppression.

N. M. S. Dos Santos, T. T. Hermsen, J. H. W. Rombout, L. Pilstroem, Rene Josephus Maria Stet

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    The coding sequence of the sea bass light chain was obtained by sequential anchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N = 17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this tow variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation. (C) 2001 Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)387-401
    Number of pages14
    JournalDevelopmental and Comparative Immunology
    Volume25
    DOIs
    Publication statusPublished - 2001

    Keywords

    • Dicentrarchus labrax
    • immunoglobulin light chain
    • expression
    • variability
    • DNP494-KLH
    • TROUT ONCORHYNCHUS-MYKISS
    • CYPRINUS-CARPIO L.
    • SALMO-SALAR L
    • RAINBOW-TROUT
    • GENOMIC ORGANIZATION
    • ANTIBODY-RESPONSES
    • CHANNEL CATFISH
    • IMMUNOGLOBULIN
    • FISH
    • GENES

    Cite this

    Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.): Evidence for intramolecular-induced suppression. / Dos Santos, N. M. S.; Hermsen, T. T.; Rombout, J. H. W.; Pilstroem, L.; Stet, Rene Josephus Maria.

    In: Developmental and Comparative Immunology, Vol. 25, 2001, p. 387-401.

    Research output: Contribution to journalArticle

    Dos Santos, N. M. S. ; Hermsen, T. T. ; Rombout, J. H. W. ; Pilstroem, L. ; Stet, Rene Josephus Maria. / Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.): Evidence for intramolecular-induced suppression. In: Developmental and Comparative Immunology. 2001 ; Vol. 25. pp. 387-401.
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    abstract = "The coding sequence of the sea bass light chain was obtained by sequential anchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N = 17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this tow variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation. (C) 2001 Elsevier Science Ltd. All rights reserved.",
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    AU - Hermsen, T. T.

    AU - Rombout, J. H. W.

    AU - Pilstroem, L.

    AU - Stet, Rene Josephus Maria

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    AB - The coding sequence of the sea bass light chain was obtained by sequential anchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N = 17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this tow variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation. (C) 2001 Elsevier Science Ltd. All rights reserved.

    KW - Dicentrarchus labrax

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    KW - variability

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    KW - TROUT ONCORHYNCHUS-MYKISS

    KW - CYPRINUS-CARPIO L.

    KW - SALMO-SALAR L

    KW - RAINBOW-TROUT

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    KW - ANTIBODY-RESPONSES

    KW - CHANNEL CATFISH

    KW - IMMUNOGLOBULIN

    KW - FISH

    KW - GENES

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