Immune-modulation of two BATF3 paralogues in rainbow trout Oncorhynchus mykiss 

Jun Wang, Min Liu, Yang Wu, Sohye Yoon, Abdo Alnabulsi, Fuguo Liu, Clara Fernández-Álvarez, Tiehui Wang, Jason W Holland, Chris J Secombes, Jun Zou

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Abstract

Basic leucine zipper transcription factor ATF-like (BATF) -3 is a member of the activator protein 1 (AP‑1) family of transcription factors and is known to play a vital role in regulating differentiation of antigen-presenting cells in mammals. In this study, two BATF3 homologues (termed BATF3a and BATF3b) have been identified in rainbow trout (Oncorhynchus mykiss). Both genes were constitutively expressed in tissues, with particularly high levels of BATF3a in spleen, liver, pyloric caecae and head kidney. BATF3a was also more highly induced by PAMPs and cytokines in cultured cells, with type II IFN a particularly potent inducer. In rIL-4/13 pre-stimulated cells, the viral PAMPS polyI:C and R848 had the most pronounced effect on BATF3 expression. BATF3 expression could also be modulated in vivo, following infection with Yersinia ruckeri, a bacterial pathogen causing redmouth disease in salmonids, or with the rhabdovirus IHNV. The results suggest that BATF3 may be functionally conserved in regulating the differentiation and activation of immune cells in lower vertebrates and could be explored as a potential marker for comparative investigation of leucocyte lineage commitment across the vertebrate phyla.

Original languageEnglish
Pages (from-to)104-114
Number of pages11
JournalMolecular Immunology
Volume99
Early online date7 May 2018
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Oncorhynchus mykiss
Vertebrates
resiquimod
Yersinia ruckeri
Infectious hematopoietic necrosis virus
Rhabdoviridae
Basic-Leucine Zipper Transcription Factors
Head Kidney
Salmonidae
Differentiation Antigens
Transcription Factor AP-1
Antigen-Presenting Cells
Mammals
Cultured Cells
Leukocytes
Transcription Factors
Spleen
Cytokines
Liver
Infection

Keywords

  • Journal Article
  • BATF3
  • transcription factor
  • leucocyte differentiation
  • bacterial and viral infection
  • rainbow trout

Cite this

Wang, J., Liu, M., Wu, Y., Yoon, S., Alnabulsi, A., Liu, F., ... Zou, J. (2018). Immune-modulation of two BATF3 paralogues in rainbow trout Oncorhynchus mykiss . Molecular Immunology, 99, 104-114. https://doi.org/10.1016/j.molimm.2018.04.016

Immune-modulation of two BATF3 paralogues in rainbow trout Oncorhynchus mykiss . / Wang, Jun; Liu, Min; Wu, Yang; Yoon, Sohye; Alnabulsi, Abdo; Liu, Fuguo; Fernández-Álvarez, Clara; Wang, Tiehui; Holland, Jason W; Secombes, Chris J; Zou, Jun.

In: Molecular Immunology, Vol. 99, 01.07.2018, p. 104-114.

Research output: Contribution to journalArticle

Wang, J, Liu, M, Wu, Y, Yoon, S, Alnabulsi, A, Liu, F, Fernández-Álvarez, C, Wang, T, Holland, JW, Secombes, CJ & Zou, J 2018, 'Immune-modulation of two BATF3 paralogues in rainbow trout Oncorhynchus mykiss ' Molecular Immunology, vol. 99, pp. 104-114. https://doi.org/10.1016/j.molimm.2018.04.016
Wang, Jun ; Liu, Min ; Wu, Yang ; Yoon, Sohye ; Alnabulsi, Abdo ; Liu, Fuguo ; Fernández-Álvarez, Clara ; Wang, Tiehui ; Holland, Jason W ; Secombes, Chris J ; Zou, Jun. / Immune-modulation of two BATF3 paralogues in rainbow trout Oncorhynchus mykiss . In: Molecular Immunology. 2018 ; Vol. 99. pp. 104-114.
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abstract = "Basic leucine zipper transcription factor ATF-like (BATF) -3 is a member of the activator protein 1 (AP‑1) family of transcription factors and is known to play a vital role in regulating differentiation of antigen-presenting cells in mammals. In this study, two BATF3 homologues (termed BATF3a and BATF3b) have been identified in rainbow trout (Oncorhynchus mykiss). Both genes were constitutively expressed in tissues, with particularly high levels of BATF3a in spleen, liver, pyloric caecae and head kidney. BATF3a was also more highly induced by PAMPs and cytokines in cultured cells, with type II IFN a particularly potent inducer. In rIL-4/13 pre-stimulated cells, the viral PAMPS polyI:C and R848 had the most pronounced effect on BATF3 expression. BATF3 expression could also be modulated in vivo, following infection with Yersinia ruckeri, a bacterial pathogen causing redmouth disease in salmonids, or with the rhabdovirus IHNV. The results suggest that BATF3 may be functionally conserved in regulating the differentiation and activation of immune cells in lower vertebrates and could be explored as a potential marker for comparative investigation of leucocyte lineage commitment across the vertebrate phyla.",
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N2 - Basic leucine zipper transcription factor ATF-like (BATF) -3 is a member of the activator protein 1 (AP‑1) family of transcription factors and is known to play a vital role in regulating differentiation of antigen-presenting cells in mammals. In this study, two BATF3 homologues (termed BATF3a and BATF3b) have been identified in rainbow trout (Oncorhynchus mykiss). Both genes were constitutively expressed in tissues, with particularly high levels of BATF3a in spleen, liver, pyloric caecae and head kidney. BATF3a was also more highly induced by PAMPs and cytokines in cultured cells, with type II IFN a particularly potent inducer. In rIL-4/13 pre-stimulated cells, the viral PAMPS polyI:C and R848 had the most pronounced effect on BATF3 expression. BATF3 expression could also be modulated in vivo, following infection with Yersinia ruckeri, a bacterial pathogen causing redmouth disease in salmonids, or with the rhabdovirus IHNV. The results suggest that BATF3 may be functionally conserved in regulating the differentiation and activation of immune cells in lower vertebrates and could be explored as a potential marker for comparative investigation of leucocyte lineage commitment across the vertebrate phyla.

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