Immunohistochemical detection of a germline BRCA1 mutation in a breast and ovarian cancer family

A C Schofield, S Payne, V G Ross, I D Miller, S D Heys, N E Haites

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tumours from four individuals in a breast and ovarian cancer family with a known deleterious germline BRCA1 mutation, were analyzed using BRCA1 antibodies. In addition, we examined tumours from 96 female patients with early-onset breast cancer, who were not selected because of any family history. Paraffin-embedded tumour sections were examined by standard immunohistochemical analysis. Three familial tumours from BRCA1 carriers displayed focal negativity. This observation was not seen in a non-mutation carrier from the same family. It was found that 9/96 (9%) early-onset breast rumours had total BRCA1 negativity. In addition, 2/2 (100%) medullary breast carcinomas displayed negativity for both antibodies. Our results indicate that BRCA1 antibodies can discriminate between familial tumours with and without a deleterious mutation from one family. Further mutation studies in early-onset breast cancer group will be necessary to evaluate the use of immunohistochemistry as a rapid, initial screening technique to identify BRCA1 mutations. (C) 2000 Harcourt Publishers Ltd.

Original languageEnglish
Pages (from-to)286-291
Number of pages6
JournalBreast
Volume9
Publication statusPublished - 2000

Keywords

  • RAPID DETECTION
  • GENE
  • CARCINOMAS
  • FEATURES
  • TUMOR

Cite this

Schofield, A. C., Payne, S., Ross, V. G., Miller, I. D., Heys, S. D., & Haites, N. E. (2000). Immunohistochemical detection of a germline BRCA1 mutation in a breast and ovarian cancer family. Breast, 9, 286-291.

Immunohistochemical detection of a germline BRCA1 mutation in a breast and ovarian cancer family. / Schofield, A C ; Payne, S ; Ross, V G ; Miller, I D ; Heys, S D ; Haites, N E .

In: Breast, Vol. 9, 2000, p. 286-291.

Research output: Contribution to journalArticle

Schofield, AC, Payne, S, Ross, VG, Miller, ID, Heys, SD & Haites, NE 2000, 'Immunohistochemical detection of a germline BRCA1 mutation in a breast and ovarian cancer family', Breast, vol. 9, pp. 286-291.
Schofield AC, Payne S, Ross VG, Miller ID, Heys SD, Haites NE. Immunohistochemical detection of a germline BRCA1 mutation in a breast and ovarian cancer family. Breast. 2000;9:286-291.
Schofield, A C ; Payne, S ; Ross, V G ; Miller, I D ; Heys, S D ; Haites, N E . / Immunohistochemical detection of a germline BRCA1 mutation in a breast and ovarian cancer family. In: Breast. 2000 ; Vol. 9. pp. 286-291.
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AU - Haites, N E

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N2 - Tumours from four individuals in a breast and ovarian cancer family with a known deleterious germline BRCA1 mutation, were analyzed using BRCA1 antibodies. In addition, we examined tumours from 96 female patients with early-onset breast cancer, who were not selected because of any family history. Paraffin-embedded tumour sections were examined by standard immunohistochemical analysis. Three familial tumours from BRCA1 carriers displayed focal negativity. This observation was not seen in a non-mutation carrier from the same family. It was found that 9/96 (9%) early-onset breast rumours had total BRCA1 negativity. In addition, 2/2 (100%) medullary breast carcinomas displayed negativity for both antibodies. Our results indicate that BRCA1 antibodies can discriminate between familial tumours with and without a deleterious mutation from one family. Further mutation studies in early-onset breast cancer group will be necessary to evaluate the use of immunohistochemistry as a rapid, initial screening technique to identify BRCA1 mutations. (C) 2000 Harcourt Publishers Ltd.

AB - Tumours from four individuals in a breast and ovarian cancer family with a known deleterious germline BRCA1 mutation, were analyzed using BRCA1 antibodies. In addition, we examined tumours from 96 female patients with early-onset breast cancer, who were not selected because of any family history. Paraffin-embedded tumour sections were examined by standard immunohistochemical analysis. Three familial tumours from BRCA1 carriers displayed focal negativity. This observation was not seen in a non-mutation carrier from the same family. It was found that 9/96 (9%) early-onset breast rumours had total BRCA1 negativity. In addition, 2/2 (100%) medullary breast carcinomas displayed negativity for both antibodies. Our results indicate that BRCA1 antibodies can discriminate between familial tumours with and without a deleterious mutation from one family. Further mutation studies in early-onset breast cancer group will be necessary to evaluate the use of immunohistochemistry as a rapid, initial screening technique to identify BRCA1 mutations. (C) 2000 Harcourt Publishers Ltd.

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KW - CARCINOMAS

KW - FEATURES

KW - TUMOR

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