Immunostaining of Skeletal Tissues

Anke J Roelofs (Corresponding Author), Cosimo De Bari

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.

Original languageEnglish
Pages (from-to)437-450
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume1914
DOIs
Publication statusPublished - 8 Feb 2019

Fingerprint

Cryoultramicrotomy
Immunohistochemistry
Paraffin Embedding
Antigens
Translational Medical Research
Waxes
Frozen Sections
Paraffin
Formaldehyde
Antibodies

Keywords

  • immunohistochemistry
  • immunofluorescence
  • paraffin section
  • cryosection
  • antigen retrieval
  • skeletal tissues
  • demineralization
  • Immunohistochemistry
  • Demineralization
  • Skeletal tissues
  • Paraffin section
  • Antigen retrieval
  • Cryosection
  • Immunofluorescence

Cite this

Immunostaining of Skeletal Tissues. / Roelofs, Anke J (Corresponding Author); De Bari, Cosimo.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1914, 08.02.2019, p. 437-450.

Research output: Contribution to journalArticle

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