Immunostaining of Skeletal Tissues

Anke J Roelofs; Cosimo De Bari

doi:10.1007/978-1-4939-8997-3_25

Immunostaining of Skeletal Tissues

Anke J Roelofs^* (Corresponding Author), Cosimo De Bari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.

Original language	English
Pages (from-to)	437-450
Number of pages	14
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1914
DOIs	https://doi.org/10.1007/978-1-4939-8997-3_25
Publication status	Published - 8 Feb 2019

Keywords

immunohistochemistry
immunofluorescence
paraffin section
cryosection
antigen retrieval
skeletal tissues
demineralization
Immunohistochemistry
Demineralization
Skeletal tissues
Paraffin section
Antigen retrieval
Cryosection
Immunofluorescence

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title = "Immunostaining of Skeletal Tissues",

abstract = "Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.",

keywords = "immunohistochemistry, immunofluorescence, paraffin section, cryosection, antigen retrieval, skeletal tissues, demineralization, Immunohistochemistry, Demineralization, Skeletal tissues, Paraffin section, Antigen retrieval, Cryosection, Immunofluorescence",

author = "Roelofs, {Anke J} and {De Bari}, Cosimo",

note = "This chapter is dedicated to the memory of Dr. Tobias Kurth, who wrote the previous edition. We are grateful for his invaluable contribution to optimizing the protocols described herein. We thank the staff of the Microscopy and Histology Facility at the Institute of Medical Sciences for their support, and Susan Clark for critical reviewing of the manuscript. We are grateful for support to our research from Arthritis Research UK (grants 19271, 19429, 20050, 20775), the Medical Research Council (grants MR/L020211/1, MR/L022893/1), and Tenovus Scotland (grant G13/14). ",

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doi = "10.1007/978-1-4939-8997-3_25",

language = "English",

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AU - Roelofs, Anke J

AU - De Bari, Cosimo

N1 - This chapter is dedicated to the memory of Dr. Tobias Kurth, who wrote the previous edition. We are grateful for his invaluable contribution to optimizing the protocols described herein. We thank the staff of the Microscopy and Histology Facility at the Institute of Medical Sciences for their support, and Susan Clark for critical reviewing of the manuscript. We are grateful for support to our research from Arthritis Research UK (grants 19271, 19429, 20050, 20775), the Medical Research Council (grants MR/L020211/1, MR/L022893/1), and Tenovus Scotland (grant G13/14).

PY - 2019/2/8

Y1 - 2019/2/8

N2 - Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.

AB - Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.

KW - immunohistochemistry

KW - immunofluorescence

KW - paraffin section

KW - cryosection

KW - antigen retrieval

KW - skeletal tissues

KW - demineralization

KW - Immunohistochemistry

KW - Demineralization

KW - Skeletal tissues

KW - Paraffin section

KW - Antigen retrieval

KW - Cryosection

KW - Immunofluorescence

U2 - 10.1007/978-1-4939-8997-3_25

DO - 10.1007/978-1-4939-8997-3_25

M3 - Article

C2 - 30729481

VL - 1914

SP - 437

EP - 450

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Immunostaining of Skeletal Tissues

Abstract

Keywords

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