Abstract
The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraporitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002% unspecified plant extract, 1% alginic acid from Laminaria digitata, and 98.998% algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses greater than or equal to1 mg ml(-1). A single dose of 1 mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at I day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the I mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined. (C) 2002 Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 101-113 |
Number of pages | 13 |
Journal | Veterinary Immunology and Immunopathology |
Volume | 86 |
Issue number | 1-2 |
Early online date | 4 Mar 2002 |
DOIs | |
Publication status | Published - May 2002 |
Keywords
- ergosan
- alginic acid
- rainbow trout
- peritoneal exudate cells
- humoral immune system
- phagocytes
- respiratory burst
- immunomodulation
- gene expression
- salmo-salar L
- nonspecific defense-mechanisms
- muscle cell-proliferation
- carp cyprinus-carpio
- aeromonas-salmonicida
- Atlantic salmon
- in-vitro
- tetraselmis-suecica
- immune-response
- sodium alginate