Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan

S Peddie, Jun Zou, Christopher John Secombes

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105 Citations (Scopus)

Abstract

The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraporitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002% unspecified plant extract, 1% alginic acid from Laminaria digitata, and 98.998% algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses greater than or equal to1 mg ml(-1). A single dose of 1 mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at I day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the I mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined. (C) 2002 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)101-113
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume86
Issue number1-2
Early online date4 Mar 2002
DOIs
Publication statusPublished - May 2002

Keywords

  • ergosan
  • alginic acid
  • rainbow trout
  • peritoneal exudate cells
  • humoral immune system
  • phagocytes
  • respiratory burst
  • immunomodulation
  • gene expression
  • salmo-salar L
  • nonspecific defense-mechanisms
  • muscle cell-proliferation
  • carp cyprinus-carpio
  • aeromonas-salmonicida
  • Atlantic salmon
  • in-vitro
  • tetraselmis-suecica
  • immune-response
  • sodium alginate

Cite this

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title = "Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan",
abstract = "The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraporitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002{\%} unspecified plant extract, 1{\%} alginic acid from Laminaria digitata, and 98.998{\%} algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses greater than or equal to1 mg ml(-1). A single dose of 1 mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at I day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the I mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined. (C) 2002 Elsevier Science B.V. All rights reserved.",
keywords = "ergosan, alginic acid, rainbow trout, peritoneal exudate cells, humoral immune system, phagocytes, respiratory burst, immunomodulation, gene expression, salmo-salar L, nonspecific defense-mechanisms, muscle cell-proliferation, carp cyprinus-carpio, aeromonas-salmonicida, Atlantic salmon, in-vitro, tetraselmis-suecica, immune-response, sodium alginate",
author = "S Peddie and Jun Zou and Secombes, {Christopher John}",
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T1 - Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan

AU - Peddie, S

AU - Zou, Jun

AU - Secombes, Christopher John

PY - 2002/5

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N2 - The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraporitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002% unspecified plant extract, 1% alginic acid from Laminaria digitata, and 98.998% algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses greater than or equal to1 mg ml(-1). A single dose of 1 mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at I day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the I mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined. (C) 2002 Elsevier Science B.V. All rights reserved.

AB - The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraporitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002% unspecified plant extract, 1% alginic acid from Laminaria digitata, and 98.998% algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses greater than or equal to1 mg ml(-1). A single dose of 1 mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at I day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the I mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined. (C) 2002 Elsevier Science B.V. All rights reserved.

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KW - humoral immune system

KW - phagocytes

KW - respiratory burst

KW - immunomodulation

KW - gene expression

KW - salmo-salar L

KW - nonspecific defense-mechanisms

KW - muscle cell-proliferation

KW - carp cyprinus-carpio

KW - aeromonas-salmonicida

KW - Atlantic salmon

KW - in-vitro

KW - tetraselmis-suecica

KW - immune-response

KW - sodium alginate

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DO - 10.1016/S0165-2427(02)00019-3

M3 - Article

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EP - 113

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

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ER -