Abstract
Purpose: Study how the dietary intake affects the fecal microbiota of a group of obese individuals after a 6-week very low-energy diet (VLED) and thereafter during a follow-up period of 5, 8, and 12 months. Additionally, we compared two different methods, fluorescent in situ hybridization (FISH) and real-time PCR (qPCR), for the quantification of fecal samples.
Methods: Sixteen subjects participated in a 12-month dietary intervention which consisted of a VLED high in protein and low in carbohydrates followed by a personalized diet plan, combined with exercise and lifestyle counseling. Fecal samples were analyzed using qPCR, FISH, and denaturing gradient gel electrophoresis.
Results: The VLED affected the fecal microbiota, in particular bifidobacteria that decreased approximately two logs compared with the baseline numbers. The change in numbers of the bacterial groups studied followed the dietary intake and not the weight variations during the 12-month intervention. Methanogens were detected in 56 % of the participants at every sampling point, regardless of the dietary intake. Moreover, although absolute numbers of comparable bacterial groups were similar between FISH and qPCR measurements, relative proportions were higher according to FISH results.
Conclusions: Changes in the fecal microbial numbers of obese individuals were primarily affected by the dietary intake rather than weight changes.
| Original language | English |
|---|---|
| Pages (from-to) | 1421-1429 |
| Number of pages | 9 |
| Journal | European Journal of Nutrition |
| Volume | 53 |
| Issue number | 6 |
| Early online date | 31 Dec 2013 |
| DOIs | |
| Publication status | Published - Sep 2014 |
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Keywords
- Human fecal microbiota
- Obesity
- Very low-energy diet
- Weight loss
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Nutrition and Dietetics
- Medicine(all)
Cite this
Impact of a very low-energy diet on the fecal microbiota of obese individuals. / Simões, C. D.; Maukonen, J.; Scott, K. P.; Virtanen, K. A.; Pietiläinen, K. H.; Saarela, M.
In: European Journal of Nutrition, Vol. 53, No. 6, 09.2014, p. 1421-1429.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Impact of a very low-energy diet on the fecal microbiota of obese individuals
AU - Simões, C. D.
AU - Maukonen, J.
AU - Scott, K. P.
AU - Virtanen, K. A.
AU - Pietiläinen, K. H.
AU - Saarela, M.
N1 - Acknowledgments Ms. Jennifer Martin and Ms. Marja-Liisa Jalovaara are greatly acknowledged for the excellent technical assistance. The authors acknowledge the support of the Portuguese Foundation for Science and Technology (FCT; Grant SFRH/BD/40920/2007) and the European Science Foundation (ESF), in the framework of the Research Networking Programme, The European Network for Gastrointestinal Health Research (ENGIHR), Helsinki and Turku University Hospital Research Funds, Finnish Diabetes Research, Novo Nordisk, Biomedicum Helsinki, Jalmari and Rauha Ahokas Foundation, and the Finnish Foundation for Cardiovascular Research.
PY - 2014/9
Y1 - 2014/9
N2 - Purpose: Study how the dietary intake affects the fecal microbiota of a group of obese individuals after a 6-week very low-energy diet (VLED) and thereafter during a follow-up period of 5, 8, and 12 months. Additionally, we compared two different methods, fluorescent in situ hybridization (FISH) and real-time PCR (qPCR), for the quantification of fecal samples. Methods: Sixteen subjects participated in a 12-month dietary intervention which consisted of a VLED high in protein and low in carbohydrates followed by a personalized diet plan, combined with exercise and lifestyle counseling. Fecal samples were analyzed using qPCR, FISH, and denaturing gradient gel electrophoresis. Results: The VLED affected the fecal microbiota, in particular bifidobacteria that decreased approximately two logs compared with the baseline numbers. The change in numbers of the bacterial groups studied followed the dietary intake and not the weight variations during the 12-month intervention. Methanogens were detected in 56 % of the participants at every sampling point, regardless of the dietary intake. Moreover, although absolute numbers of comparable bacterial groups were similar between FISH and qPCR measurements, relative proportions were higher according to FISH results. Conclusions: Changes in the fecal microbial numbers of obese individuals were primarily affected by the dietary intake rather than weight changes.
AB - Purpose: Study how the dietary intake affects the fecal microbiota of a group of obese individuals after a 6-week very low-energy diet (VLED) and thereafter during a follow-up period of 5, 8, and 12 months. Additionally, we compared two different methods, fluorescent in situ hybridization (FISH) and real-time PCR (qPCR), for the quantification of fecal samples. Methods: Sixteen subjects participated in a 12-month dietary intervention which consisted of a VLED high in protein and low in carbohydrates followed by a personalized diet plan, combined with exercise and lifestyle counseling. Fecal samples were analyzed using qPCR, FISH, and denaturing gradient gel electrophoresis. Results: The VLED affected the fecal microbiota, in particular bifidobacteria that decreased approximately two logs compared with the baseline numbers. The change in numbers of the bacterial groups studied followed the dietary intake and not the weight variations during the 12-month intervention. Methanogens were detected in 56 % of the participants at every sampling point, regardless of the dietary intake. Moreover, although absolute numbers of comparable bacterial groups were similar between FISH and qPCR measurements, relative proportions were higher according to FISH results. Conclusions: Changes in the fecal microbial numbers of obese individuals were primarily affected by the dietary intake rather than weight changes.
KW - Human fecal microbiota
KW - Obesity
KW - Very low-energy diet
KW - Weight loss
UR - http://www.scopus.com/inward/record.url?scp=84906938884&partnerID=8YFLogxK
U2 - 10.1007/s00394-013-0645-0
DO - 10.1007/s00394-013-0645-0
M3 - Article
C2 - 24378982
AN - SCOPUS:84906938884
VL - 53
SP - 1421
EP - 1429
JO - European Journal of Nutrition
JF - European Journal of Nutrition
SN - 1436-6207
IS - 6
ER -