Influence of ammonia concentration on 15N-ammonia incorporation and de novo amino acid synthesis by the non-cellulolytic ruminal bacteria, Prevotella bryantii B14, Streptococcus bovis ES1 and Selenomonas ruminantium HD4

C. Atasoglu, R. J. Wallace

Research output: Contribution to journalArticle

Abstract

The influence of ammonia concentration on ^{15}N-ammonia incorporation and de novo synthesis of amino acids by three predominant non-cellulolytic species of ruminal bacteria, Prevotella bryantii B_{1}4, Selenomonas ruminantium HD4 and Streptococcus bovis ES1, was investigated. The medium contained pancreatic casein hydrolysate (comprising mainly peptides with some amino acids) at a concentration of 1 g/litre and additions of graded concentrations of 15NH4Cl. When the initial concentration of ammonia increased from 0.045 to 0.436 g N/L in the growth medium, the proportion of cellular nitrogen and amino acid nitrogen derived from ammonia by P. bryantii and S. ruminantium increased (ranging from 0.33 to 0.84 for cellular-nitrogen and from 0.23 to 0.67 for amino acid-nitrogen) (P<0.001), but S. bovis incorporated a fixed proportion of ammonia and peptides in all media except for the lowest ammonia containing medium (P>0.05). Glutamate and aspartate were the most highly labelled amino acids with ^{15}N, whereas ^{15}N enrichment in proline was lower than that in other amino acids in all species, followed by phenylalanine in P. bryantii, lysine in S. ruminantium and phenylalanine, valine and lysine in S. bovis, indicating preferential incorporation of these amino acids from pancreatic casein hydrolysate. The results of the present study, thus, suggest that the concentration of ammonia has an important effect on de novo synthesis of bacterial cellular-nitrogen and amino acids in the non-cellulolytic ruminal bacteria and this effect depends on bacterial species.
Original languageEnglish
Pages (from-to)389-395
Number of pages7
JournalTurkish Journal of Veterinary Animal Science
Volume26
Issue number2
Publication statusPublished - 2002

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Prevotella bryantii
Selenomonas ruminantium
Streptococcus bovis
rumen bacteria
ammonia
amino acids
synthesis
nitrogen
casein hydrolysates
phenylalanine
lysine
aspartic acid
valine
glutamates
proline
culture media
peptides

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@article{a891f75baaf44b2bad57245f29222d75,
title = "Influence of ammonia concentration on 15N-ammonia incorporation and de novo amino acid synthesis by the non-cellulolytic ruminal bacteria, Prevotella bryantii B14, Streptococcus bovis ES1 and Selenomonas ruminantium HD4",
abstract = "The influence of ammonia concentration on ^{15}N-ammonia incorporation and de novo synthesis of amino acids by three predominant non-cellulolytic species of ruminal bacteria, Prevotella bryantii B_{1}4, Selenomonas ruminantium HD4 and Streptococcus bovis ES1, was investigated. The medium contained pancreatic casein hydrolysate (comprising mainly peptides with some amino acids) at a concentration of 1 g/litre and additions of graded concentrations of 15NH4Cl. When the initial concentration of ammonia increased from 0.045 to 0.436 g N/L in the growth medium, the proportion of cellular nitrogen and amino acid nitrogen derived from ammonia by P. bryantii and S. ruminantium increased (ranging from 0.33 to 0.84 for cellular-nitrogen and from 0.23 to 0.67 for amino acid-nitrogen) (P<0.001), but S. bovis incorporated a fixed proportion of ammonia and peptides in all media except for the lowest ammonia containing medium (P>0.05). Glutamate and aspartate were the most highly labelled amino acids with ^{15}N, whereas ^{15}N enrichment in proline was lower than that in other amino acids in all species, followed by phenylalanine in P. bryantii, lysine in S. ruminantium and phenylalanine, valine and lysine in S. bovis, indicating preferential incorporation of these amino acids from pancreatic casein hydrolysate. The results of the present study, thus, suggest that the concentration of ammonia has an important effect on de novo synthesis of bacterial cellular-nitrogen and amino acids in the non-cellulolytic ruminal bacteria and this effect depends on bacterial species.",
author = "C. Atasoglu and Wallace, {R. J.}",
note = "No spares;",
year = "2002",
language = "English",
volume = "26",
pages = "389--395",
journal = "Turkish Journal of Veterinary Animal Science",
number = "2",

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TY - JOUR

T1 - Influence of ammonia concentration on 15N-ammonia incorporation and de novo amino acid synthesis by the non-cellulolytic ruminal bacteria, Prevotella bryantii B14, Streptococcus bovis ES1 and Selenomonas ruminantium HD4

AU - Atasoglu, C.

AU - Wallace, R. J.

N1 - No spares;

PY - 2002

Y1 - 2002

N2 - The influence of ammonia concentration on ^{15}N-ammonia incorporation and de novo synthesis of amino acids by three predominant non-cellulolytic species of ruminal bacteria, Prevotella bryantii B_{1}4, Selenomonas ruminantium HD4 and Streptococcus bovis ES1, was investigated. The medium contained pancreatic casein hydrolysate (comprising mainly peptides with some amino acids) at a concentration of 1 g/litre and additions of graded concentrations of 15NH4Cl. When the initial concentration of ammonia increased from 0.045 to 0.436 g N/L in the growth medium, the proportion of cellular nitrogen and amino acid nitrogen derived from ammonia by P. bryantii and S. ruminantium increased (ranging from 0.33 to 0.84 for cellular-nitrogen and from 0.23 to 0.67 for amino acid-nitrogen) (P<0.001), but S. bovis incorporated a fixed proportion of ammonia and peptides in all media except for the lowest ammonia containing medium (P>0.05). Glutamate and aspartate were the most highly labelled amino acids with ^{15}N, whereas ^{15}N enrichment in proline was lower than that in other amino acids in all species, followed by phenylalanine in P. bryantii, lysine in S. ruminantium and phenylalanine, valine and lysine in S. bovis, indicating preferential incorporation of these amino acids from pancreatic casein hydrolysate. The results of the present study, thus, suggest that the concentration of ammonia has an important effect on de novo synthesis of bacterial cellular-nitrogen and amino acids in the non-cellulolytic ruminal bacteria and this effect depends on bacterial species.

AB - The influence of ammonia concentration on ^{15}N-ammonia incorporation and de novo synthesis of amino acids by three predominant non-cellulolytic species of ruminal bacteria, Prevotella bryantii B_{1}4, Selenomonas ruminantium HD4 and Streptococcus bovis ES1, was investigated. The medium contained pancreatic casein hydrolysate (comprising mainly peptides with some amino acids) at a concentration of 1 g/litre and additions of graded concentrations of 15NH4Cl. When the initial concentration of ammonia increased from 0.045 to 0.436 g N/L in the growth medium, the proportion of cellular nitrogen and amino acid nitrogen derived from ammonia by P. bryantii and S. ruminantium increased (ranging from 0.33 to 0.84 for cellular-nitrogen and from 0.23 to 0.67 for amino acid-nitrogen) (P<0.001), but S. bovis incorporated a fixed proportion of ammonia and peptides in all media except for the lowest ammonia containing medium (P>0.05). Glutamate and aspartate were the most highly labelled amino acids with ^{15}N, whereas ^{15}N enrichment in proline was lower than that in other amino acids in all species, followed by phenylalanine in P. bryantii, lysine in S. ruminantium and phenylalanine, valine and lysine in S. bovis, indicating preferential incorporation of these amino acids from pancreatic casein hydrolysate. The results of the present study, thus, suggest that the concentration of ammonia has an important effect on de novo synthesis of bacterial cellular-nitrogen and amino acids in the non-cellulolytic ruminal bacteria and this effect depends on bacterial species.

M3 - Article

VL - 26

SP - 389

EP - 395

JO - Turkish Journal of Veterinary Animal Science

JF - Turkish Journal of Veterinary Animal Science

IS - 2

ER -