Influence of culture E(h) on the growth and metabolism of the rumen bacteria Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and Streptococcus bovis in batch culture

M. Marounek, R. J. Wallace

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Abstract

One facultatively and three strictly anaerobic rumen bacteria were grown in pH-controlled anaerobic batch cultures in which the E h of the medium was regulated by the addition of titanium (III) citrate solution at values below −50 mV and potassium ferricyanide above −50 mV. Growth occurred over a wide range of E h, with the maximum limit being +360, + 250, +175 and +414 mV for Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and the aerotolerant Streptococcus bovis, respectively. Changes in E h had little influence on the growth yield or ratios of fermentation end-products in these bacteria over a fairly wide range, although the specific growth rate of all species tended to decline at E h values above 0 mV. Abnormal, elongated forms of Sel. ruminantium and B. succinogenes predominated at high E h. It was concluded that O2, and not a high E h, is the toxic factor in “oxidized” anaerobic growth medium, and that it would not normally be necessary to regulate E h closely in experiments where the growth and metabolism of these bacteria is under study, provided that O2-free conditions were maintained.
Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalJournal of General Microbiology
Volume130
Issue number2
DOIs
Publication statusPublished - Feb 1984

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Selenomonas
Streptococcus bovis
Bacteroides
Batch Cell Culture Techniques
Rumen
Bacteria
Growth
Anaerobic Bacteria
Poisons
Fermentation

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title = "Influence of culture E(h) on the growth and metabolism of the rumen bacteria Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and Streptococcus bovis in batch culture",
abstract = "One facultatively and three strictly anaerobic rumen bacteria were grown in pH-controlled anaerobic batch cultures in which the E h of the medium was regulated by the addition of titanium (III) citrate solution at values below −50 mV and potassium ferricyanide above −50 mV. Growth occurred over a wide range of E h, with the maximum limit being +360, + 250, +175 and +414 mV for Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and the aerotolerant Streptococcus bovis, respectively. Changes in E h had little influence on the growth yield or ratios of fermentation end-products in these bacteria over a fairly wide range, although the specific growth rate of all species tended to decline at E h values above 0 mV. Abnormal, elongated forms of Sel. ruminantium and B. succinogenes predominated at high E h. It was concluded that O2, and not a high E h, is the toxic factor in “oxidized” anaerobic growth medium, and that it would not normally be necessary to regulate E h closely in experiments where the growth and metabolism of these bacteria is under study, provided that O2-free conditions were maintained.",
author = "M. Marounek and Wallace, {R. J.}",
note = "Copyright 2004 Elsevier B.V., All rights reserved.",
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AU - Wallace, R. J.

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N2 - One facultatively and three strictly anaerobic rumen bacteria were grown in pH-controlled anaerobic batch cultures in which the E h of the medium was regulated by the addition of titanium (III) citrate solution at values below −50 mV and potassium ferricyanide above −50 mV. Growth occurred over a wide range of E h, with the maximum limit being +360, + 250, +175 and +414 mV for Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and the aerotolerant Streptococcus bovis, respectively. Changes in E h had little influence on the growth yield or ratios of fermentation end-products in these bacteria over a fairly wide range, although the specific growth rate of all species tended to decline at E h values above 0 mV. Abnormal, elongated forms of Sel. ruminantium and B. succinogenes predominated at high E h. It was concluded that O2, and not a high E h, is the toxic factor in “oxidized” anaerobic growth medium, and that it would not normally be necessary to regulate E h closely in experiments where the growth and metabolism of these bacteria is under study, provided that O2-free conditions were maintained.

AB - One facultatively and three strictly anaerobic rumen bacteria were grown in pH-controlled anaerobic batch cultures in which the E h of the medium was regulated by the addition of titanium (III) citrate solution at values below −50 mV and potassium ferricyanide above −50 mV. Growth occurred over a wide range of E h, with the maximum limit being +360, + 250, +175 and +414 mV for Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and the aerotolerant Streptococcus bovis, respectively. Changes in E h had little influence on the growth yield or ratios of fermentation end-products in these bacteria over a fairly wide range, although the specific growth rate of all species tended to decline at E h values above 0 mV. Abnormal, elongated forms of Sel. ruminantium and B. succinogenes predominated at high E h. It was concluded that O2, and not a high E h, is the toxic factor in “oxidized” anaerobic growth medium, and that it would not normally be necessary to regulate E h closely in experiments where the growth and metabolism of these bacteria is under study, provided that O2-free conditions were maintained.

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