Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity

A. J. Roe, C. O'Byrne, Debbie McLaggan, Ian Rylance Booth

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

The mechanism by which methionine relieves the growth inhibition of Escherichia coli K-12 that is caused by organic weak acid food preservatives was investigated. In the presence of 8 mM acetate the specific growth rate of E. coli Frag1 (in MaclIvaine's minimal medium pH 6.0) is reduced by 50%. Addition of methionine restores growth to 80% of that observed in untreated controls. Similar relief was seen with cultures treated with either benzoate or propionate. Mutants with an elevated intracellular methionine pool were almost completely resistant to the inhibitory effects of acetate, suggesting that the methionine pool becomes limiting for growth in acetate-treated cells. Measurement of the intracellular concentrations of pathway intermediates revealed that the homocysteine pool is increased dramatically in acetate-treated cells, suggesting that acetate inhibits a biosynthetic step downstream from this intermediate. Supplementation of the medium with homocysteine inhibits the growth of E. coli cells. Acetate inhibition of growth arises from the depletion of the intracellular methionine pool with the concomitant accumulation of the toxic intermediate homocysteine and this augments the effect of lowering cytoplasmic pH.

Original languageEnglish
Pages (from-to)2215-2222
Number of pages7
JournalMicrobiology
Volume148
Issue numberPt 7
Publication statusPublished - 2002

Keywords

  • enteric bacteria
  • amino acid synthesis
  • acetate
  • weak acids
  • metabolite toxicity
  • WEAK ACIDS
  • POTASSIUM-TRANSPORT
  • FOOD PRESERVATIVES
  • LIPOPHILIC ACIDS
  • CYTOPLASMIC PH
  • SORBIC ACID
  • BACTERIA
  • K-12
  • EXPRESSION
  • METABOLISM

Cite this

Roe, A. J., O'Byrne, C., McLaggan, D., & Booth, I. R. (2002). Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity. Microbiology , 148(Pt 7), 2215-2222.

Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity. / Roe, A. J.; O'Byrne, C.; McLaggan, Debbie; Booth, Ian Rylance.

In: Microbiology , Vol. 148, No. Pt 7, 2002, p. 2215-2222.

Research output: Contribution to journalArticle

Roe, AJ, O'Byrne, C, McLaggan, D & Booth, IR 2002, 'Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity', Microbiology , vol. 148, no. Pt 7, pp. 2215-2222.
Roe, A. J. ; O'Byrne, C. ; McLaggan, Debbie ; Booth, Ian Rylance. / Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity. In: Microbiology . 2002 ; Vol. 148, No. Pt 7. pp. 2215-2222.
@article{56c9b9a8c0ae461589617f4611b14364,
title = "Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity",
abstract = "The mechanism by which methionine relieves the growth inhibition of Escherichia coli K-12 that is caused by organic weak acid food preservatives was investigated. In the presence of 8 mM acetate the specific growth rate of E. coli Frag1 (in MaclIvaine's minimal medium pH 6.0) is reduced by 50{\%}. Addition of methionine restores growth to 80{\%} of that observed in untreated controls. Similar relief was seen with cultures treated with either benzoate or propionate. Mutants with an elevated intracellular methionine pool were almost completely resistant to the inhibitory effects of acetate, suggesting that the methionine pool becomes limiting for growth in acetate-treated cells. Measurement of the intracellular concentrations of pathway intermediates revealed that the homocysteine pool is increased dramatically in acetate-treated cells, suggesting that acetate inhibits a biosynthetic step downstream from this intermediate. Supplementation of the medium with homocysteine inhibits the growth of E. coli cells. Acetate inhibition of growth arises from the depletion of the intracellular methionine pool with the concomitant accumulation of the toxic intermediate homocysteine and this augments the effect of lowering cytoplasmic pH.",
keywords = "enteric bacteria, amino acid synthesis, acetate, weak acids, metabolite toxicity, WEAK ACIDS, POTASSIUM-TRANSPORT, FOOD PRESERVATIVES, LIPOPHILIC ACIDS, CYTOPLASMIC PH, SORBIC ACID, BACTERIA, K-12, EXPRESSION, METABOLISM",
author = "Roe, {A. J.} and C. O'Byrne and Debbie McLaggan and Booth, {Ian Rylance}",
year = "2002",
language = "English",
volume = "148",
pages = "2215--2222",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "Pt 7",

}

TY - JOUR

T1 - Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity

AU - Roe, A. J.

AU - O'Byrne, C.

AU - McLaggan, Debbie

AU - Booth, Ian Rylance

PY - 2002

Y1 - 2002

N2 - The mechanism by which methionine relieves the growth inhibition of Escherichia coli K-12 that is caused by organic weak acid food preservatives was investigated. In the presence of 8 mM acetate the specific growth rate of E. coli Frag1 (in MaclIvaine's minimal medium pH 6.0) is reduced by 50%. Addition of methionine restores growth to 80% of that observed in untreated controls. Similar relief was seen with cultures treated with either benzoate or propionate. Mutants with an elevated intracellular methionine pool were almost completely resistant to the inhibitory effects of acetate, suggesting that the methionine pool becomes limiting for growth in acetate-treated cells. Measurement of the intracellular concentrations of pathway intermediates revealed that the homocysteine pool is increased dramatically in acetate-treated cells, suggesting that acetate inhibits a biosynthetic step downstream from this intermediate. Supplementation of the medium with homocysteine inhibits the growth of E. coli cells. Acetate inhibition of growth arises from the depletion of the intracellular methionine pool with the concomitant accumulation of the toxic intermediate homocysteine and this augments the effect of lowering cytoplasmic pH.

AB - The mechanism by which methionine relieves the growth inhibition of Escherichia coli K-12 that is caused by organic weak acid food preservatives was investigated. In the presence of 8 mM acetate the specific growth rate of E. coli Frag1 (in MaclIvaine's minimal medium pH 6.0) is reduced by 50%. Addition of methionine restores growth to 80% of that observed in untreated controls. Similar relief was seen with cultures treated with either benzoate or propionate. Mutants with an elevated intracellular methionine pool were almost completely resistant to the inhibitory effects of acetate, suggesting that the methionine pool becomes limiting for growth in acetate-treated cells. Measurement of the intracellular concentrations of pathway intermediates revealed that the homocysteine pool is increased dramatically in acetate-treated cells, suggesting that acetate inhibits a biosynthetic step downstream from this intermediate. Supplementation of the medium with homocysteine inhibits the growth of E. coli cells. Acetate inhibition of growth arises from the depletion of the intracellular methionine pool with the concomitant accumulation of the toxic intermediate homocysteine and this augments the effect of lowering cytoplasmic pH.

KW - enteric bacteria

KW - amino acid synthesis

KW - acetate

KW - weak acids

KW - metabolite toxicity

KW - WEAK ACIDS

KW - POTASSIUM-TRANSPORT

KW - FOOD PRESERVATIVES

KW - LIPOPHILIC ACIDS

KW - CYTOPLASMIC PH

KW - SORBIC ACID

KW - BACTERIA

KW - K-12

KW - EXPRESSION

KW - METABOLISM

M3 - Article

VL - 148

SP - 2215

EP - 2222

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - Pt 7

ER -