Inhibitory effect of the anorexic compound oleoylethanolamide on gastric emptying in control and overweight mice

Gabriella Aviello; Isabel Matias; Raffaele Capasso; Stefania Petrosino; Francesca Borrelli; Pierangelo Orlando; Barbara Romano; Francesco Capasso; Vincenzo Di Marzo; Angelo A Izzo

doi:10.1007/s00109-008-0305-7

Inhibitory effect of the anorexic compound oleoylethanolamide on gastric emptying in control and overweight mice

Gabriella Aviello, Isabel Matias, Raffaele Capasso, Stefania Petrosino, Francesca Borrelli, Pierangelo Orlando, Barbara Romano, Francesco Capasso, Vincenzo Di Marzo, Angelo A Izzo

The Rowett Institute of Nutrition and Health

University of Naples Federico II

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Gastric emptying regulates food intake. Oleoylethanolamide (OEA), an endogenous acylethanolamide chemically related to the endocannabinoid anandamide, inhibits food intake, but its effect on gastric emptying is unknown. Here, we investigated the effect and the role of OEA on gastric emptying in mice fed either a standard (STD) or a high-fat diet (HFD) for 14 weeks. Gastric emptying was reduced by OEA, but not by its saturated analog, palmitoylethanolamide. The effect of OEA was unaffected by rimonabant (cannabinoid CB1 receptor antagonist), SR144528 (cannabinoid CB2 receptor antagonist), 5'-iodoresiniferatoxin (transient receptor potential vanilloid type 1 antagonist), or MK886 (peroxisome proliferator-activated receptor-alpha) antagonist. Compared to STD mice, HFD mice showed delayed gastric emptying and higher levels of gastric OEA. HFD-induced increase in OEA levels was accompanied by increased expression of the OEA-synthesizing enzyme N-acyl-phosphatidylethanolamine-selective phospholipase D and decreased expression of the OEA-degrading enzyme fatty acid amide hydrolase. These results might suggest that elevation of gastric OEA could possibly contribute to the delayed gastric emptying observed in HFD-fed animals. HFD regulates OEA levels in the stomach through an increase of its biosynthesis and a decrease of its enzymatic degradation. The inhibitory effect of OEA on gastric emptying here observed might underlie part of the anorexic effects of this compound previously reported.

Original language	English
Pages (from-to)	413-422
Number of pages	10
Journal	Journal of Molecular Medicine
Volume	86
Issue number	4
Early online date	16 Feb 2008
DOIs	https://doi.org/10.1007/s00109-008-0305-7
Publication status	Published - Apr 2008

Bibliographical note

This work was supported by Prin, Regione Campania, “Fondazione Enrico ed Enrica Sovena,” Epitech, S.r.l. (to SP and VDM), and Sanofi-Aventis (to VDM).

Keywords

Animals
Appetite Depressants
Cannabinoids
Eating
Endocannabinoids
Ethanolamines
Gastric Emptying
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Oleic Acids
Palmitic Acids
Journal Article
Research Support, Non-U.S. Gov't

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title = "Inhibitory effect of the anorexic compound oleoylethanolamide on gastric emptying in control and overweight mice",

abstract = "Gastric emptying regulates food intake. Oleoylethanolamide (OEA), an endogenous acylethanolamide chemically related to the endocannabinoid anandamide, inhibits food intake, but its effect on gastric emptying is unknown. Here, we investigated the effect and the role of OEA on gastric emptying in mice fed either a standard (STD) or a high-fat diet (HFD) for 14 weeks. Gastric emptying was reduced by OEA, but not by its saturated analog, palmitoylethanolamide. The effect of OEA was unaffected by rimonabant (cannabinoid CB1 receptor antagonist), SR144528 (cannabinoid CB2 receptor antagonist), 5'-iodoresiniferatoxin (transient receptor potential vanilloid type 1 antagonist), or MK886 (peroxisome proliferator-activated receptor-alpha) antagonist. Compared to STD mice, HFD mice showed delayed gastric emptying and higher levels of gastric OEA. HFD-induced increase in OEA levels was accompanied by increased expression of the OEA-synthesizing enzyme N-acyl-phosphatidylethanolamine-selective phospholipase D and decreased expression of the OEA-degrading enzyme fatty acid amide hydrolase. These results might suggest that elevation of gastric OEA could possibly contribute to the delayed gastric emptying observed in HFD-fed animals. HFD regulates OEA levels in the stomach through an increase of its biosynthesis and a decrease of its enzymatic degradation. The inhibitory effect of OEA on gastric emptying here observed might underlie part of the anorexic effects of this compound previously reported.",

keywords = "Animals, Appetite Depressants, Cannabinoids, Eating, Endocannabinoids, Ethanolamines, Gastric Emptying, Male, Mice, Mice, Inbred C57BL, Mice, Obese, Oleic Acids, Palmitic Acids, Journal Article, Research Support, Non-U.S. Gov't",

author = "Gabriella Aviello and Isabel Matias and Raffaele Capasso and Stefania Petrosino and Francesca Borrelli and Pierangelo Orlando and Barbara Romano and Francesco Capasso and {Di Marzo}, Vincenzo and Izzo, {Angelo A}",

note = "This work was supported by Prin, Regione Campania, “Fondazione Enrico ed Enrica Sovena,” Epitech, S.r.l. (to SP and VDM), and Sanofi-Aventis (to VDM). ",

year = "2008",

month = apr,

doi = "10.1007/s00109-008-0305-7",

language = "English",

volume = "86",

pages = "413--422",

journal = "Journal of Molecular Medicine",

issn = "0946-2716",

publisher = "Springer Verlag",

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T1 - Inhibitory effect of the anorexic compound oleoylethanolamide on gastric emptying in control and overweight mice

AU - Aviello, Gabriella

AU - Matias, Isabel

AU - Capasso, Raffaele

AU - Petrosino, Stefania

AU - Borrelli, Francesca

AU - Orlando, Pierangelo

AU - Romano, Barbara

AU - Capasso, Francesco

AU - Di Marzo, Vincenzo

AU - Izzo, Angelo A

N1 - This work was supported by Prin, Regione Campania, “Fondazione Enrico ed Enrica Sovena,” Epitech, S.r.l. (to SP and VDM), and Sanofi-Aventis (to VDM).

PY - 2008/4

Y1 - 2008/4

N2 - Gastric emptying regulates food intake. Oleoylethanolamide (OEA), an endogenous acylethanolamide chemically related to the endocannabinoid anandamide, inhibits food intake, but its effect on gastric emptying is unknown. Here, we investigated the effect and the role of OEA on gastric emptying in mice fed either a standard (STD) or a high-fat diet (HFD) for 14 weeks. Gastric emptying was reduced by OEA, but not by its saturated analog, palmitoylethanolamide. The effect of OEA was unaffected by rimonabant (cannabinoid CB1 receptor antagonist), SR144528 (cannabinoid CB2 receptor antagonist), 5'-iodoresiniferatoxin (transient receptor potential vanilloid type 1 antagonist), or MK886 (peroxisome proliferator-activated receptor-alpha) antagonist. Compared to STD mice, HFD mice showed delayed gastric emptying and higher levels of gastric OEA. HFD-induced increase in OEA levels was accompanied by increased expression of the OEA-synthesizing enzyme N-acyl-phosphatidylethanolamine-selective phospholipase D and decreased expression of the OEA-degrading enzyme fatty acid amide hydrolase. These results might suggest that elevation of gastric OEA could possibly contribute to the delayed gastric emptying observed in HFD-fed animals. HFD regulates OEA levels in the stomach through an increase of its biosynthesis and a decrease of its enzymatic degradation. The inhibitory effect of OEA on gastric emptying here observed might underlie part of the anorexic effects of this compound previously reported.

AB - Gastric emptying regulates food intake. Oleoylethanolamide (OEA), an endogenous acylethanolamide chemically related to the endocannabinoid anandamide, inhibits food intake, but its effect on gastric emptying is unknown. Here, we investigated the effect and the role of OEA on gastric emptying in mice fed either a standard (STD) or a high-fat diet (HFD) for 14 weeks. Gastric emptying was reduced by OEA, but not by its saturated analog, palmitoylethanolamide. The effect of OEA was unaffected by rimonabant (cannabinoid CB1 receptor antagonist), SR144528 (cannabinoid CB2 receptor antagonist), 5'-iodoresiniferatoxin (transient receptor potential vanilloid type 1 antagonist), or MK886 (peroxisome proliferator-activated receptor-alpha) antagonist. Compared to STD mice, HFD mice showed delayed gastric emptying and higher levels of gastric OEA. HFD-induced increase in OEA levels was accompanied by increased expression of the OEA-synthesizing enzyme N-acyl-phosphatidylethanolamine-selective phospholipase D and decreased expression of the OEA-degrading enzyme fatty acid amide hydrolase. These results might suggest that elevation of gastric OEA could possibly contribute to the delayed gastric emptying observed in HFD-fed animals. HFD regulates OEA levels in the stomach through an increase of its biosynthesis and a decrease of its enzymatic degradation. The inhibitory effect of OEA on gastric emptying here observed might underlie part of the anorexic effects of this compound previously reported.

KW - Animals

KW - Appetite Depressants

KW - Cannabinoids

KW - Eating

KW - Endocannabinoids

KW - Ethanolamines

KW - Gastric Emptying

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Obese

KW - Oleic Acids

KW - Palmitic Acids

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00109-008-0305-7

DO - 10.1007/s00109-008-0305-7

M3 - Article

C2 - 18278475

SN - 0946-2716

VL - 86

SP - 413

EP - 422

JO - Journal of Molecular Medicine

JF - Journal of Molecular Medicine

IS - 4

ER -

Inhibitory effect of the anorexic compound oleoylethanolamide on gastric emptying in control and overweight mice

Abstract

Bibliographical note

Keywords

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