Abstract
Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.
Original language | English |
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Pages (from-to) | 33-44 |
Number of pages | 12 |
Journal | Physiological Genomics |
Volume | 32 |
Issue number | 1 |
Early online date | 5 Sept 2007 |
DOIs | |
Publication status | Published - 19 Dec 2007 |
Keywords
- transcriptome
- immune
- fish
- trout oncorhynchus-mykiss
- double-stranded-RNA
- Rainbow-trout
- expression analysis
- CDNA microarray
- molecular-cloning
- DNA vaccination
- necrosis virus
- CXC chemokine
- fugu-rubripes