Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray

S. A. M. Martin, J. B. Taggart, P. Seear, J. E. Bron, R. Talbot, A. J. Teale, G. E. Sweeney, B. Hoyheim, D. F. Houlihan, D. R. Tocher, J. Zou, C. J. Secombes

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Abstract

Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.

Original languageEnglish
Pages (from-to)33-44
Number of pages12
JournalPhysiological Genomics
Volume32
Issue number1
Early online date5 Sep 2007
DOIs
Publication statusPublished - 19 Dec 2007

Keywords

  • transcriptome
  • immune
  • fish
  • trout oncorhynchus-mykiss
  • double-stranded-RNA
  • Rainbow-trout
  • expression analysis
  • CDNA microarray
  • molecular-cloning
  • DNA vaccination
  • necrosis virus
  • CXC chemokine
  • fugu-rubripes

Cite this

Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray. / Martin, S. A. M.; Taggart, J. B.; Seear, P.; Bron, J. E.; Talbot, R.; Teale, A. J.; Sweeney, G. E.; Hoyheim, B.; Houlihan, D. F.; Tocher, D. R.; Zou, J.; Secombes, C. J.

In: Physiological Genomics, Vol. 32, No. 1, 19.12.2007, p. 33-44.

Research output: Contribution to journalArticle

Martin, SAM, Taggart, JB, Seear, P, Bron, JE, Talbot, R, Teale, AJ, Sweeney, GE, Hoyheim, B, Houlihan, DF, Tocher, DR, Zou, J & Secombes, CJ 2007, 'Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray', Physiological Genomics, vol. 32, no. 1, pp. 33-44. https://doi.org/10.1152/physiolgenomics.00064.2007
Martin, S. A. M. ; Taggart, J. B. ; Seear, P. ; Bron, J. E. ; Talbot, R. ; Teale, A. J. ; Sweeney, G. E. ; Hoyheim, B. ; Houlihan, D. F. ; Tocher, D. R. ; Zou, J. ; Secombes, C. J. / Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray. In: Physiological Genomics. 2007 ; Vol. 32, No. 1. pp. 33-44.
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abstract = "Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.",
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AU - Bron, J. E.

AU - Talbot, R.

AU - Teale, A. J.

AU - Sweeney, G. E.

AU - Hoyheim, B.

AU - Houlihan, D. F.

AU - Tocher, D. R.

AU - Zou, J.

AU - Secombes, C. J.

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AB - Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.

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KW - double-stranded-RNA

KW - Rainbow-trout

KW - expression analysis

KW - CDNA microarray

KW - molecular-cloning

KW - DNA vaccination

KW - necrosis virus

KW - CXC chemokine

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