Internuclear gene silencing in Phytophthora infestans

P van West, S Kamoun, J W van 't Klooster, F Govers

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Transformation of the diploid oomycete plant pathogen Phytophthora infestans with antisense, sense, and promoter-less constructs of the coding sequence of the elicitin gene inf1 resulted in transcriptional silencing of both the transgenes and the endogenous gene. Since heterokaryons obtained by somatic fusion of an inf1-silenced transgenic strain and a wild-type strain displayed stable gene silencing, inf1 silencing is dominant and acts in trans, Inf1 remained silenced in nontransgenic homokaryotic progeny from the silenced heterokaryons, thereby demonstrating that the presence of transgenes is not essential for maintaining the silenced status of the endogenous inf1 gene. These findings support a model reminiscent of paramutation and involving a trans-acting factor that is capable of transferring a silencing signal between nuclei.

Original languageEnglish
Pages (from-to)339-348
Number of pages10
JournalMolecular Cell
Volume3
Publication statusPublished - 1999

Keywords

  • TRANSGENIC PLANTS
  • EPIGENETIC PHENOMENA
  • NEUROSPORA-CRASSA
  • FILAMENTOUS FUNGI
  • VIRUS-RESISTANCE
  • DNA METHYLATION
  • PARAMUTATION
  • SUPPRESSION
  • EXPRESSION
  • COSUPPRESSION

Cite this

van West, P., Kamoun, S., van 't Klooster, J. W., & Govers, F. (1999). Internuclear gene silencing in Phytophthora infestans. Molecular Cell, 3, 339-348.