Isolation and characterization of 10 microsatellite loci in the common dormouse Muscardinus avellanarius

Darlina Md. Naim, Stephen J. Kemp, Sandra Elizabeth Telfer, Phillip C. Watts

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Abstract

To assess the level and spatial pattern of genetic diversity of the common dormouse, Muscardinus avellanarius, we developed polymorphic microsatellite loci from partial genomic libraries enriched for microsatellite motifs. Ten dinucleotide polymorphic microsatellites were isolated and levels of genetic diversity were assessed in 139 individuals from Bontuchel, (Denbighshire, Wales). We observed high levels of heterozygosity (mean H-O = 0.792 +/- 0.077; mean H-E = 0.730 +/- 0.084) and a large number of alleles (N-a = 6-17). There was no evidence of deviations from Hardy-Weinberg conditions, or genotypic disequilibrium between any pairs of loci. This is the first description of microsatellite primers from a common dormouse and these loci are currently being used to quantify dormouse spatial genetic structure.

Original languageEnglish
Pages (from-to)1010-1012
Number of pages3
JournalMolecular Ecology Resources
Volume9
Issue number3
Early online date20 Feb 2009
DOIs
Publication statusPublished - May 2009

Keywords

  • conservation
  • microsatellites
  • Muscardinus avellanarius
  • population structure
  • DNA

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