Isolation of hyperglycaemic peptides from the corpus cardiacum of the american cockroach, Periplaneta americana

Karl J. Siegert*, Peter J. Morgan, William Mordue

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two peptides, HGHI and HGHII, have been isolated from the CC (corpus cardiacum) of the American cockroach, Periplaneta americana, both showing hyperglycaemic and phosphorylase activating potency when tested in adult cockroaches. The isolation procedure involved extraction of CC with 80% methanol and two steps of HPLC (high-performance liquid chromatography). In the first step extracted material was eluted in 0.1% TFA (trifluoroacetic acid) from a molecular size exclusion column. Estimates of molecular mass indicate that both peptides have a molecular mass of about 1000 daltons. In the second step reversed-phase HPLC utilizing a solvent system consisting of 0.1% TFA and acetonitrile was used; a gradient starting at 25% acetonitrile, with a slope 0.3% acetonitrile min-1 was run over 25 min. The first major peak, HGHI, eluted after 13.7 min and caused a strong hyperglycaemic response as well as strong activation of fat body glycogen phosphorylase when the equivalent of 0.1 of a pair was tested. For HGHII eluting after 21.3 min the hyperglycaemic effect as well as phosphorylase activation were only 50% of the response obtained for HGHI when the equivalent of 0.1 of a pair was injected.

Original languageEnglish
Pages (from-to)365-371
Number of pages7
JournalInsect Biochemistry
Volume16
Issue number2
DOIs
Publication statusPublished - 1 Jan 1986

Keywords

  • corpora cardiaca
  • fat body glycogen phosphorylase
  • haemolymph carbohydrates
  • HGHI
  • HGHII
  • HPLC
  • Hyperglycaemic peptides
  • Periplaneta americana

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