Lens-regulated retinoic acid signalling controls expansion of the developing eye

Jonathan N Smith, Heather M Walker, Hannah Thompson, J. Martin Collinson, Neil Vargesson, Lynda Erskine (Corresponding Author)

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Absence of the developing lens results in severe eye defects, including substantial reductions in eye size. How the lens controls eye expansion and the underlying signalling pathways are very poorly defined. We identified RDH10, a gene crucial for retinoic acid synthesis during embryogenesis, as a key factor downregulated in the peripheral retina (presumptive ciliary body region) of lens-removed embryonic chicken eyes prior to overt reductions in eye size. This is associated with a significant decrease in retinoic acid synthesis by lens-removed eyes. Restoring retinoic acid signalling in lens-removed eyes by implanting beads soaked in retinoic acid or retinal, but not vitamin A, rescued eye size. Conversely, blocking retinoic acid synthesis decreased eye size in lens-containing eyes. Production of collagen II and collagen IX, which are major vitreal proteins, is also regulated by the lens and retinoic acid signalling. These data mechanistically link the known roles of both the lens and retinoic acid in normal eye development, and support a model whereby retinoic acid production by the peripheral retina acts downstream of the lens to support vitreous production and eye expansion.
Original languageEnglish
Article numberdev167171
JournalDevelopment
Volume145
Issue number19
DOIs
Publication statusPublished - 10 Oct 2018

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Tretinoin
Lenses
Crystalline Lens
Retina
Collagen
Body Regions
Ciliary Body
Vitamin A
Embryonic Development
Chickens
Down-Regulation
Genes

Keywords

  • ciliary body
  • vitreous
  • collagen II
  • collagen IX
  • RDH10
  • Vitamin A

Cite this

Lens-regulated retinoic acid signalling controls expansion of the developing eye. / Smith, Jonathan N; Walker, Heather M; Thompson, Hannah; Collinson, J. Martin; Vargesson, Neil; Erskine, Lynda (Corresponding Author).

In: Development, Vol. 145, No. 19, dev167171, 10.10.2018.

Research output: Contribution to journalArticle

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