Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier

H. Xu, R. Dawson, I. J. Crane, Janet Mary Liversidge

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

PURPOSE. Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial.

METHODS. Confocal immunofluorescence microscopy of ex vivo retinal wholemounts was used to study BRB integrity during leukocyte adhesion and migration during experimental autoimmune uveoretinitis (EAU). Western blot analysis was used to measure levels of TJ proteins in EAU retina and RPE and in normal retina or RPE cultured with cytokines or chemokines.

RESULTS. No evidence for discontinuity or other weakness of the endothelial or epithelial barrier at tricellular corners was observed, and maximum disruption of TJ protein expression was focused in retinal venules correlating with sites of leukocyte extravasation. Areas of maximum TJ protein loss in vivo also correlated with redistribution or loss of ensheathing astrocyte processes on venules but not adjacent capillaries or arterioles. Exposure of normal choroidal and retinal explants ex vivo to cytokines and chemokines alone did not downregulate total occludin-1 or claudin-3 TJ protein expression.

CONCLUSIONS. The data presented herein support an active role for leukocytes in TJ disruption and blood-retina barrier (BRB) breakdown during retinal inflammation and further implicate venule microenvironment as a key factor in leukocyte recruitment to retinal tissue in vivo.

Original languageEnglish
Pages (from-to)2487-2494
Number of pages7
JournalInvestigative Ophthalmology & Visual Science
Volume46
Issue number7
DOIs
Publication statusPublished - Jul 2005

Keywords

  • experimental autoimmune uveoretinitis
  • pigment epithelial-cells
  • central-nervous-system
  • brain-barrier
  • endothelial-cells
  • multiple-sclerosis
  • growth-factor
  • TNF-Alpha
  • expression
  • occludin

Cite this

Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier. / Xu, H.; Dawson, R.; Crane, I. J.; Liversidge, Janet Mary.

In: Investigative Ophthalmology & Visual Science, Vol. 46, No. 7, 07.2005, p. 2487-2494.

Research output: Contribution to journalArticle

Xu, H. ; Dawson, R. ; Crane, I. J. ; Liversidge, Janet Mary. / Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier. In: Investigative Ophthalmology & Visual Science. 2005 ; Vol. 46, No. 7. pp. 2487-2494.
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N2 - PURPOSE. Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial.METHODS. Confocal immunofluorescence microscopy of ex vivo retinal wholemounts was used to study BRB integrity during leukocyte adhesion and migration during experimental autoimmune uveoretinitis (EAU). Western blot analysis was used to measure levels of TJ proteins in EAU retina and RPE and in normal retina or RPE cultured with cytokines or chemokines.RESULTS. No evidence for discontinuity or other weakness of the endothelial or epithelial barrier at tricellular corners was observed, and maximum disruption of TJ protein expression was focused in retinal venules correlating with sites of leukocyte extravasation. Areas of maximum TJ protein loss in vivo also correlated with redistribution or loss of ensheathing astrocyte processes on venules but not adjacent capillaries or arterioles. Exposure of normal choroidal and retinal explants ex vivo to cytokines and chemokines alone did not downregulate total occludin-1 or claudin-3 TJ protein expression.CONCLUSIONS. The data presented herein support an active role for leukocytes in TJ disruption and blood-retina barrier (BRB) breakdown during retinal inflammation and further implicate venule microenvironment as a key factor in leukocyte recruitment to retinal tissue in vivo.

AB - PURPOSE. Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial.METHODS. Confocal immunofluorescence microscopy of ex vivo retinal wholemounts was used to study BRB integrity during leukocyte adhesion and migration during experimental autoimmune uveoretinitis (EAU). Western blot analysis was used to measure levels of TJ proteins in EAU retina and RPE and in normal retina or RPE cultured with cytokines or chemokines.RESULTS. No evidence for discontinuity or other weakness of the endothelial or epithelial barrier at tricellular corners was observed, and maximum disruption of TJ protein expression was focused in retinal venules correlating with sites of leukocyte extravasation. Areas of maximum TJ protein loss in vivo also correlated with redistribution or loss of ensheathing astrocyte processes on venules but not adjacent capillaries or arterioles. Exposure of normal choroidal and retinal explants ex vivo to cytokines and chemokines alone did not downregulate total occludin-1 or claudin-3 TJ protein expression.CONCLUSIONS. The data presented herein support an active role for leukocytes in TJ disruption and blood-retina barrier (BRB) breakdown during retinal inflammation and further implicate venule microenvironment as a key factor in leukocyte recruitment to retinal tissue in vivo.

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