Measurement of the rate of substrate cycling between acetate and acetyl-CoA in sheep muscle in vivo. Effects of infusion of acetate

B Crabtree, S A Marr, Susan Elizabeth Anderson, J C MacRae

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

1. The rate of substrate cycling between acetate and acetyl-CoA in sheep hindlimb muscle was measured from the decrease of [14C]acetate specific radioactivity across the tissue in vivo. 2. The rate of cycling was 0.005-0.015 mumol/min per g and, in two of the three sheep investigated, was approximately doubled as a result of infusing 2 mol acetate/day intravenously. 3. The results indicate that this cycle contributes only approx. 0.5% of the total heat produced by the animal and would probably make only a relatively small contribution to any heat increment of feeding associated with diets producing large amounts of acetate.
Original languageEnglish
Pages (from-to)821-827
Number of pages7
JournalBiochemical Journal
Volume243
Issue number3
DOIs
Publication statusPublished - 1 May 1987

Fingerprint

Substrate Cycling
Acetyl Coenzyme A
Muscle
Sheep
Acetates
Muscles
Substrates
Hot Temperature
Radioactivity
Hindlimb
Nutrition
Enthalpy
Animals
Tissue
Diet

Keywords

  • Acetic Acid
  • Acetic Acids
  • Acetyl Coenzyme A
  • Animals
  • Body Temperature Regulation
  • Citric Acid Cycle
  • Male
  • Muscles
  • Sheep

Cite this

Measurement of the rate of substrate cycling between acetate and acetyl-CoA in sheep muscle in vivo. Effects of infusion of acetate. / Crabtree, B; Marr, S A; Anderson, Susan Elizabeth; MacRae, J C.

In: Biochemical Journal, Vol. 243, No. 3, 01.05.1987, p. 821-827.

Research output: Contribution to journalArticle

Crabtree, B ; Marr, S A ; Anderson, Susan Elizabeth ; MacRae, J C. / Measurement of the rate of substrate cycling between acetate and acetyl-CoA in sheep muscle in vivo. Effects of infusion of acetate. In: Biochemical Journal. 1987 ; Vol. 243, No. 3. pp. 821-827.
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AB - 1. The rate of substrate cycling between acetate and acetyl-CoA in sheep hindlimb muscle was measured from the decrease of [14C]acetate specific radioactivity across the tissue in vivo. 2. The rate of cycling was 0.005-0.015 mumol/min per g and, in two of the three sheep investigated, was approximately doubled as a result of infusing 2 mol acetate/day intravenously. 3. The results indicate that this cycle contributes only approx. 0.5% of the total heat produced by the animal and would probably make only a relatively small contribution to any heat increment of feeding associated with diets producing large amounts of acetate.

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