Measurement of the rate of substrate cycling between acetate and acetyl-CoA in sheep muscle in vivo. Effects of infusion of acetate

B Crabtree, S A Marr, Susan Elizabeth Anderson, J C MacRae

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8 Citations (Scopus)

Abstract

1. The rate of substrate cycling between acetate and acetyl-CoA in sheep hindlimb muscle was measured from the decrease of [14C]acetate specific radioactivity across the tissue in vivo. 2. The rate of cycling was 0.005-0.015 mumol/min per g and, in two of the three sheep investigated, was approximately doubled as a result of infusing 2 mol acetate/day intravenously. 3. The results indicate that this cycle contributes only approx. 0.5% of the total heat produced by the animal and would probably make only a relatively small contribution to any heat increment of feeding associated with diets producing large amounts of acetate.
Original languageEnglish
Pages (from-to)821-827
Number of pages7
JournalBiochemical Journal
Volume243
Issue number3
DOIs
Publication statusPublished - 1 May 1987

Keywords

  • Acetic Acid
  • Acetic Acids
  • Acetyl Coenzyme A
  • Animals
  • Body Temperature Regulation
  • Citric Acid Cycle
  • Male
  • Muscles
  • Sheep

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