Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae

J M Sharples, A A Meharg, S M Chambers, J W G Cairney

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Arsenate resistance is exhibited by the ericoid mycorrhizal fungus Hymenoscyphus ericae collected from As-contaminated mine soils. To investigate the mechanism of arsenate resistance, uptake kinetics for arsenate (H2AsO4-), arsenite (H3AsO3), and phosphate (H2PrO4-) were determined in both arsenate-resistant and -non-resistant H. ericae. The uptake kinetics of H2AsO4-, H3AsO3, and H2PO4- in both resistant and non-resistant isolates were similar. The presence of 5.0 muM H2PO4- repressed uptake of H2AsO4- and exposure to 0.75 mM H2AsO4- repressed H2PO4- uptake in both H. ericae. Mine site H. ericae demonstrated an enhanced As efflux mechanism in comparison with non-resistant H, ericae and lost approximately 90%; of preloaded cellular As (1-h uptake of 0.22 mu mol g(-1) dry weight h(-1) H2AsO4-) over a 5-h period in comparison with non-resistant: H. ericae, which lost 40% of their total absorbed H2AsO4-. As lost from the fungal tissue was in the form of H3AsO3. The results of the present study demonstrate an enhanced H2AsO4- efflux system operating in mine site H. ericae as a mechanism for H2AsO4- resistance. The ecological significance of this mechanism of arsenate resistance is discussed.

Original languageEnglish
Pages (from-to)1327-1334
Number of pages8
JournalPlant Physiology
Volume124
Issue number3
Publication statusPublished - Nov 2000

Keywords

  • PHOSPHATE-UPTAKE SYSTEM
  • HOLCUS-LANATUS L
  • PHOSPHORUS
  • DEFICIENCY
  • TRANSPORT
  • KINETICS
  • PLANTS
  • ROOTS
  • ABSORPTION
  • TOLERANCE

Cite this

Sharples, J. M., Meharg, A. A., Chambers, S. M., & Cairney, J. W. G. (2000). Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. Plant Physiology, 124(3), 1327-1334.

Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. / Sharples, J M ; Meharg, A A ; Chambers, S M ; Cairney, J W G .

In: Plant Physiology, Vol. 124, No. 3, 11.2000, p. 1327-1334.

Research output: Contribution to journalArticle

Sharples, JM, Meharg, AA, Chambers, SM & Cairney, JWG 2000, 'Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae' Plant Physiology, vol. 124, no. 3, pp. 1327-1334.
Sharples JM, Meharg AA, Chambers SM, Cairney JWG. Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. Plant Physiology. 2000 Nov;124(3):1327-1334.
Sharples, J M ; Meharg, A A ; Chambers, S M ; Cairney, J W G . / Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. In: Plant Physiology. 2000 ; Vol. 124, No. 3. pp. 1327-1334.
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abstract = "Arsenate resistance is exhibited by the ericoid mycorrhizal fungus Hymenoscyphus ericae collected from As-contaminated mine soils. To investigate the mechanism of arsenate resistance, uptake kinetics for arsenate (H2AsO4-), arsenite (H3AsO3), and phosphate (H2PrO4-) were determined in both arsenate-resistant and -non-resistant H. ericae. The uptake kinetics of H2AsO4-, H3AsO3, and H2PO4- in both resistant and non-resistant isolates were similar. The presence of 5.0 muM H2PO4- repressed uptake of H2AsO4- and exposure to 0.75 mM H2AsO4- repressed H2PO4- uptake in both H. ericae. Mine site H. ericae demonstrated an enhanced As efflux mechanism in comparison with non-resistant H, ericae and lost approximately 90{\%}; of preloaded cellular As (1-h uptake of 0.22 mu mol g(-1) dry weight h(-1) H2AsO4-) over a 5-h period in comparison with non-resistant: H. ericae, which lost 40{\%} of their total absorbed H2AsO4-. As lost from the fungal tissue was in the form of H3AsO3. The results of the present study demonstrate an enhanced H2AsO4- efflux system operating in mine site H. ericae as a mechanism for H2AsO4- resistance. The ecological significance of this mechanism of arsenate resistance is discussed.",
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T1 - Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae

AU - Sharples, J M

AU - Meharg, A A

AU - Chambers, S M

AU - Cairney, J W G

PY - 2000/11

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N2 - Arsenate resistance is exhibited by the ericoid mycorrhizal fungus Hymenoscyphus ericae collected from As-contaminated mine soils. To investigate the mechanism of arsenate resistance, uptake kinetics for arsenate (H2AsO4-), arsenite (H3AsO3), and phosphate (H2PrO4-) were determined in both arsenate-resistant and -non-resistant H. ericae. The uptake kinetics of H2AsO4-, H3AsO3, and H2PO4- in both resistant and non-resistant isolates were similar. The presence of 5.0 muM H2PO4- repressed uptake of H2AsO4- and exposure to 0.75 mM H2AsO4- repressed H2PO4- uptake in both H. ericae. Mine site H. ericae demonstrated an enhanced As efflux mechanism in comparison with non-resistant H, ericae and lost approximately 90%; of preloaded cellular As (1-h uptake of 0.22 mu mol g(-1) dry weight h(-1) H2AsO4-) over a 5-h period in comparison with non-resistant: H. ericae, which lost 40% of their total absorbed H2AsO4-. As lost from the fungal tissue was in the form of H3AsO3. The results of the present study demonstrate an enhanced H2AsO4- efflux system operating in mine site H. ericae as a mechanism for H2AsO4- resistance. The ecological significance of this mechanism of arsenate resistance is discussed.

AB - Arsenate resistance is exhibited by the ericoid mycorrhizal fungus Hymenoscyphus ericae collected from As-contaminated mine soils. To investigate the mechanism of arsenate resistance, uptake kinetics for arsenate (H2AsO4-), arsenite (H3AsO3), and phosphate (H2PrO4-) were determined in both arsenate-resistant and -non-resistant H. ericae. The uptake kinetics of H2AsO4-, H3AsO3, and H2PO4- in both resistant and non-resistant isolates were similar. The presence of 5.0 muM H2PO4- repressed uptake of H2AsO4- and exposure to 0.75 mM H2AsO4- repressed H2PO4- uptake in both H. ericae. Mine site H. ericae demonstrated an enhanced As efflux mechanism in comparison with non-resistant H, ericae and lost approximately 90%; of preloaded cellular As (1-h uptake of 0.22 mu mol g(-1) dry weight h(-1) H2AsO4-) over a 5-h period in comparison with non-resistant: H. ericae, which lost 40% of their total absorbed H2AsO4-. As lost from the fungal tissue was in the form of H3AsO3. The results of the present study demonstrate an enhanced H2AsO4- efflux system operating in mine site H. ericae as a mechanism for H2AsO4- resistance. The ecological significance of this mechanism of arsenate resistance is discussed.

KW - PHOSPHATE-UPTAKE SYSTEM

KW - HOLCUS-LANATUS L

KW - PHOSPHORUS

KW - DEFICIENCY

KW - TRANSPORT

KW - KINETICS

KW - PLANTS

KW - ROOTS

KW - ABSORPTION

KW - TOLERANCE

M3 - Article

VL - 124

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EP - 1334

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -