Melatonin suppresses the induction of AP-1 transcription factor components in the pars tuberalis of the pituitary

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Abstract

In ovine pars tuberalis cells which express high affinity Mel la melatonin receptors, the ability of melatonin to directly stimulate or inhibit AP-1 transcription factor gene expression was studied. Effects of melatonin upon mRNA expression by forskolin, serum and IGF-1 were also investigated. Northern analysis showed melatonin had no direct stimulatory nor inhibitory effect upon transcription or translation. Melatonin was able to significantly inhibit forskolin-stimulated induction of c-fos and jun B mRNA whilst forskolin had no effect upon c-jun or jun D. Induction of c-Fos translation by forskolin was also inhibited by melatonin. Serum induced c-fos and c-jun, but melatonin was unable to affect these changes. Similarly IGF-1 stimulated c-fos and melatonin had no effect upon this induction. From these results it can be concluded that melatonin has no independent effects on expression of the AP-1 genes, rather its primary function is to inhibit cell activities through cyclic AMP-dependent routes of gene activation.

Original languageEnglish
Pages (from-to)71-80
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume123
Issue number1
Publication statusPublished - 14 Oct 1996

Keywords

  • melatonin
  • pars tuberalis
  • gene expression
  • c-fos
  • AP-1
  • pituitary
  • ovine pituitary
  • adenylate-cyclase
  • protein-kinase
  • FOS proteins
  • cells
  • secretion
  • prolactin
  • signal
  • acid
  • Jun

Cite this

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title = "Melatonin suppresses the induction of AP-1 transcription factor components in the pars tuberalis of the pituitary",
abstract = "In ovine pars tuberalis cells which express high affinity Mel la melatonin receptors, the ability of melatonin to directly stimulate or inhibit AP-1 transcription factor gene expression was studied. Effects of melatonin upon mRNA expression by forskolin, serum and IGF-1 were also investigated. Northern analysis showed melatonin had no direct stimulatory nor inhibitory effect upon transcription or translation. Melatonin was able to significantly inhibit forskolin-stimulated induction of c-fos and jun B mRNA whilst forskolin had no effect upon c-jun or jun D. Induction of c-Fos translation by forskolin was also inhibited by melatonin. Serum induced c-fos and c-jun, but melatonin was unable to affect these changes. Similarly IGF-1 stimulated c-fos and melatonin had no effect upon this induction. From these results it can be concluded that melatonin has no independent effects on expression of the AP-1 genes, rather its primary function is to inhibit cell activities through cyclic AMP-dependent routes of gene activation.",
keywords = "melatonin, pars tuberalis, gene expression, c-fos, AP-1, pituitary, ovine pituitary, adenylate-cyclase, protein-kinase, FOS proteins, cells, secretion, prolactin, signal, acid, Jun",
author = "Alexander Ross and Perry Barrett and Julian Mercer and Morgan, {Peter John}",
year = "1996",
month = "10",
day = "14",
language = "English",
volume = "123",
pages = "71--80",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1",

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TY - JOUR

T1 - Melatonin suppresses the induction of AP-1 transcription factor components in the pars tuberalis of the pituitary

AU - Ross, Alexander

AU - Barrett, Perry

AU - Mercer, Julian

AU - Morgan, Peter John

PY - 1996/10/14

Y1 - 1996/10/14

N2 - In ovine pars tuberalis cells which express high affinity Mel la melatonin receptors, the ability of melatonin to directly stimulate or inhibit AP-1 transcription factor gene expression was studied. Effects of melatonin upon mRNA expression by forskolin, serum and IGF-1 were also investigated. Northern analysis showed melatonin had no direct stimulatory nor inhibitory effect upon transcription or translation. Melatonin was able to significantly inhibit forskolin-stimulated induction of c-fos and jun B mRNA whilst forskolin had no effect upon c-jun or jun D. Induction of c-Fos translation by forskolin was also inhibited by melatonin. Serum induced c-fos and c-jun, but melatonin was unable to affect these changes. Similarly IGF-1 stimulated c-fos and melatonin had no effect upon this induction. From these results it can be concluded that melatonin has no independent effects on expression of the AP-1 genes, rather its primary function is to inhibit cell activities through cyclic AMP-dependent routes of gene activation.

AB - In ovine pars tuberalis cells which express high affinity Mel la melatonin receptors, the ability of melatonin to directly stimulate or inhibit AP-1 transcription factor gene expression was studied. Effects of melatonin upon mRNA expression by forskolin, serum and IGF-1 were also investigated. Northern analysis showed melatonin had no direct stimulatory nor inhibitory effect upon transcription or translation. Melatonin was able to significantly inhibit forskolin-stimulated induction of c-fos and jun B mRNA whilst forskolin had no effect upon c-jun or jun D. Induction of c-Fos translation by forskolin was also inhibited by melatonin. Serum induced c-fos and c-jun, but melatonin was unable to affect these changes. Similarly IGF-1 stimulated c-fos and melatonin had no effect upon this induction. From these results it can be concluded that melatonin has no independent effects on expression of the AP-1 genes, rather its primary function is to inhibit cell activities through cyclic AMP-dependent routes of gene activation.

KW - melatonin

KW - pars tuberalis

KW - gene expression

KW - c-fos

KW - AP-1

KW - pituitary

KW - ovine pituitary

KW - adenylate-cyclase

KW - protein-kinase

KW - FOS proteins

KW - cells

KW - secretion

KW - prolactin

KW - signal

KW - acid

KW - Jun

M3 - Article

VL - 123

SP - 71

EP - 80

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1

ER -