Metabolic properties of Eubacterium pyruvativorans, a ruminal 'hyper-ammonia-producing' anaerobe with metabolic properties analogous to those of Clostridium kluyveri

R. John Wallace, Lal C. Chaudhary, Eiichi Miyagawa, Nest McKain, Nicola D. Walker

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

wEubacterium pyruvativorans 1-6(T) is a non-saccharolytic, arnino-acid-fermenting anaerobe from the rumen, isolated by its ability to grow on pancreatic casein hydrolysate (PCH) as sole C source. This study investigated its metabolic properties and its likely ecological niche. Additional growth was supported by pyruvate, vinyl acetate, and, to a lesser extent, lactate and crotonate, and also by a mixture of amino acids (alanine, glycine, serine and threonine) predicted to be catabolized to pyruvate. No single amino acid supported growth, and peptides were required for growth on amino acids. Alanine, followed by leucine, serine and proline, were used most extensively during growth, but only alanine and asparate were extensively modified before incorporation. Growth on PCH, but not on pyruvate, was increased by the addition of acetate, propionate and butyrate. L-Lactate was fermented incompletely, mainly to acetate, but no laclate-C was incorporated. Propionate and butyrate were utilized during growth, forming valerate and caproate, respectively. Labelling experiments suggested a metabolic pattern where two C atoms of butyrate, valerate and caproate were derived from amino acids, with the others being formed from acetate, propionate and butyrate. The metabolic strategy of E pyruvativorans therefore resembles that of Clostridium kluyveri, which ferments ethanol only when the reaction is coupled to acetate, propionate or butyrate utilization. The fermentative niche of E pyruvativorans appears to be to scavenge amino acids, lactate and possibly other metabolites in order to generate ATIP via acetate formation, using volatile fatty acid elongation with C-2 units derived from other substrates to dispose of reducing equivalents.

Original languageEnglish
Pages (from-to)2921-2930
Number of pages10
JournalMicrobiology
Volume150
Issue number9
DOIs
Publication statusPublished - Sep 2004

Keywords

  • amino acids
  • Selenomonas ruminantium
  • SP-NOV
  • bacteria
  • rumen
  • fermentation
  • Peptostreptococcus
  • monensin
  • peptides
  • sheep

Cite this

Metabolic properties of Eubacterium pyruvativorans, a ruminal 'hyper-ammonia-producing' anaerobe with metabolic properties analogous to those of Clostridium kluyveri. / Wallace, R. John; Chaudhary, Lal C.; Miyagawa, Eiichi; McKain, Nest; Walker, Nicola D.

In: Microbiology , Vol. 150, No. 9, 09.2004, p. 2921-2930.

Research output: Contribution to journalArticle

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title = "Metabolic properties of Eubacterium pyruvativorans, a ruminal 'hyper-ammonia-producing' anaerobe with metabolic properties analogous to those of Clostridium kluyveri",
abstract = "wEubacterium pyruvativorans 1-6(T) is a non-saccharolytic, arnino-acid-fermenting anaerobe from the rumen, isolated by its ability to grow on pancreatic casein hydrolysate (PCH) as sole C source. This study investigated its metabolic properties and its likely ecological niche. Additional growth was supported by pyruvate, vinyl acetate, and, to a lesser extent, lactate and crotonate, and also by a mixture of amino acids (alanine, glycine, serine and threonine) predicted to be catabolized to pyruvate. No single amino acid supported growth, and peptides were required for growth on amino acids. Alanine, followed by leucine, serine and proline, were used most extensively during growth, but only alanine and asparate were extensively modified before incorporation. Growth on PCH, but not on pyruvate, was increased by the addition of acetate, propionate and butyrate. L-Lactate was fermented incompletely, mainly to acetate, but no laclate-C was incorporated. Propionate and butyrate were utilized during growth, forming valerate and caproate, respectively. Labelling experiments suggested a metabolic pattern where two C atoms of butyrate, valerate and caproate were derived from amino acids, with the others being formed from acetate, propionate and butyrate. The metabolic strategy of E pyruvativorans therefore resembles that of Clostridium kluyveri, which ferments ethanol only when the reaction is coupled to acetate, propionate or butyrate utilization. The fermentative niche of E pyruvativorans appears to be to scavenge amino acids, lactate and possibly other metabolites in order to generate ATIP via acetate formation, using volatile fatty acid elongation with C-2 units derived from other substrates to dispose of reducing equivalents.",
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AB - wEubacterium pyruvativorans 1-6(T) is a non-saccharolytic, arnino-acid-fermenting anaerobe from the rumen, isolated by its ability to grow on pancreatic casein hydrolysate (PCH) as sole C source. This study investigated its metabolic properties and its likely ecological niche. Additional growth was supported by pyruvate, vinyl acetate, and, to a lesser extent, lactate and crotonate, and also by a mixture of amino acids (alanine, glycine, serine and threonine) predicted to be catabolized to pyruvate. No single amino acid supported growth, and peptides were required for growth on amino acids. Alanine, followed by leucine, serine and proline, were used most extensively during growth, but only alanine and asparate were extensively modified before incorporation. Growth on PCH, but not on pyruvate, was increased by the addition of acetate, propionate and butyrate. L-Lactate was fermented incompletely, mainly to acetate, but no laclate-C was incorporated. Propionate and butyrate were utilized during growth, forming valerate and caproate, respectively. Labelling experiments suggested a metabolic pattern where two C atoms of butyrate, valerate and caproate were derived from amino acids, with the others being formed from acetate, propionate and butyrate. The metabolic strategy of E pyruvativorans therefore resembles that of Clostridium kluyveri, which ferments ethanol only when the reaction is coupled to acetate, propionate or butyrate utilization. The fermentative niche of E pyruvativorans appears to be to scavenge amino acids, lactate and possibly other metabolites in order to generate ATIP via acetate formation, using volatile fatty acid elongation with C-2 units derived from other substrates to dispose of reducing equivalents.

KW - amino acids

KW - Selenomonas ruminantium

KW - SP-NOV

KW - bacteria

KW - rumen

KW - fermentation

KW - Peptostreptococcus

KW - monensin

KW - peptides

KW - sheep

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SN - 1350-0872

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