Metabolism of halogenated bisphosphonates by the cellular slime mould Dictyostelium discoideum

Michael John Rogers, R G Russell, G M Blackburn, M P Williamson, D J Watts

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Methylenebisphosphonate and its monofluoro-, difluoro- and dichloro- derivatives inhibited growth of amoebae of Dictyostelium discoideum. Dichloromethylenebisphosphonate was the most potent inhibitor of amoebal growth whereas difluoromethylenebisphosphonate was the least potent inhibitor. Each of the bisphosphonates was taken up by the amoebae and incorporated into the corresponding beta, gamma-methylene analogue of adenosine triphosphate. Two of the bisphosphonates were also incorporated into the corresponding analogues of diadenosyl tetraphosphate. No correlation was found between the ability of the bisphosphonates to inhibit amoebal growth and the extent to which they were metabolised.
Original languageEnglish
Pages (from-to)414-23
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume189
Issue number1
Publication statusPublished - 30 Nov 1992

Fingerprint

Dictyosteliida
Dictyostelium
Diphosphonates
Metabolism
Amoeba
Clodronic Acid
Growth Inhibitors
Growth
Adenosine Triphosphate
Derivatives

Keywords

  • Adenosine Triphosphate
  • Animals
  • Biological Transport
  • Dictyostelium
  • Diphosphates
  • Diphosphonates
  • Kinetics
  • Magnetic Resonance Spectroscopy
  • Structure-Activity Relationship

Cite this

Rogers, M. J., Russell, R. G., Blackburn, G. M., Williamson, M. P., & Watts, D. J. (1992). Metabolism of halogenated bisphosphonates by the cellular slime mould Dictyostelium discoideum. Biochemical and Biophysical Research Communications, 189(1), 414-23.

Metabolism of halogenated bisphosphonates by the cellular slime mould Dictyostelium discoideum. / Rogers, Michael John; Russell, R G; Blackburn, G M; Williamson, M P; Watts, D J.

In: Biochemical and Biophysical Research Communications, Vol. 189, No. 1, 30.11.1992, p. 414-23.

Research output: Contribution to journalArticle

Rogers, MJ, Russell, RG, Blackburn, GM, Williamson, MP & Watts, DJ 1992, 'Metabolism of halogenated bisphosphonates by the cellular slime mould Dictyostelium discoideum', Biochemical and Biophysical Research Communications, vol. 189, no. 1, pp. 414-23.
Rogers, Michael John ; Russell, R G ; Blackburn, G M ; Williamson, M P ; Watts, D J. / Metabolism of halogenated bisphosphonates by the cellular slime mould Dictyostelium discoideum. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 189, No. 1. pp. 414-23.
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AB - Methylenebisphosphonate and its monofluoro-, difluoro- and dichloro- derivatives inhibited growth of amoebae of Dictyostelium discoideum. Dichloromethylenebisphosphonate was the most potent inhibitor of amoebal growth whereas difluoromethylenebisphosphonate was the least potent inhibitor. Each of the bisphosphonates was taken up by the amoebae and incorporated into the corresponding beta, gamma-methylene analogue of adenosine triphosphate. Two of the bisphosphonates were also incorporated into the corresponding analogues of diadenosyl tetraphosphate. No correlation was found between the ability of the bisphosphonates to inhibit amoebal growth and the extent to which they were metabolised.

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