Mining complex bacteria media for all fluorinated compounds made possible by using HPLC Coupled Parallel to Fluorine-Specific and Molecular Specific Detection

Zhiwei Qin, Andrea Raab, Eva Krupp, Hai Deng (Corresponding Author), Jörg Feldmann (Corresponding Author)

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6 Citations (Scopus)

Abstract

The lack of specific fluorine detection at trace levels hampers the screening of microorganisms which can form novel fluorometabolites. So far only target analysis by ESI-MS using accurate mass and certain fragmentation or preconcentration and clean-up procedures has been used to perform 19F and 13C/1H NMR for the identification of novel fluorocompounds. Here we demonstrate that the analysis of complex media from microorganism cultures can be screened using a set of HPLC separations which are coupled parallel to CS-MAS as a fluorine specific detector and the ESI-MS as the molecular detection. This makes it possible to identify fluorine containing species without prior knowledge of the compounds and the use of clean up procedures. Here specifically we identify traces of a fluorometabolite (5′-fluoro-5′-deoxy-adenosine, 5′-FDA) in complex media of Streptomyces cattleya without preconcentration and target analysis.
Original languageEnglish
Pages (from-to)877-882
Number of pages6
JournalJournal of Analytical Atomic Spectrometry
Volume28
Issue number6
DOIs
Publication statusPublished - 15 Mar 2013

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Fluorine
Bacteria
Microorganisms
Adenosine
Screening
Nuclear magnetic resonance
Detectors

Cite this

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title = "Mining complex bacteria media for all fluorinated compounds made possible by using HPLC Coupled Parallel to Fluorine-Specific and Molecular Specific Detection",
abstract = "The lack of specific fluorine detection at trace levels hampers the screening of microorganisms which can form novel fluorometabolites. So far only target analysis by ESI-MS using accurate mass and certain fragmentation or preconcentration and clean-up procedures has been used to perform 19F and 13C/1H NMR for the identification of novel fluorocompounds. Here we demonstrate that the analysis of complex media from microorganism cultures can be screened using a set of HPLC separations which are coupled parallel to CS-MAS as a fluorine specific detector and the ESI-MS as the molecular detection. This makes it possible to identify fluorine containing species without prior knowledge of the compounds and the use of clean up procedures. Here specifically we identify traces of a fluorometabolite (5′-fluoro-5′-deoxy-adenosine, 5′-FDA) in complex media of Streptomyces cattleya without preconcentration and target analysis.",
author = "Zhiwei Qin and Andrea Raab and Eva Krupp and Hai Deng and J{\"o}rg Feldmann",
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T1 - Mining complex bacteria media for all fluorinated compounds made possible by using HPLC Coupled Parallel to Fluorine-Specific and Molecular Specific Detection

AU - Qin, Zhiwei

AU - Raab, Andrea

AU - Krupp, Eva

AU - Deng, Hai

AU - Feldmann, Jörg

PY - 2013/3/15

Y1 - 2013/3/15

N2 - The lack of specific fluorine detection at trace levels hampers the screening of microorganisms which can form novel fluorometabolites. So far only target analysis by ESI-MS using accurate mass and certain fragmentation or preconcentration and clean-up procedures has been used to perform 19F and 13C/1H NMR for the identification of novel fluorocompounds. Here we demonstrate that the analysis of complex media from microorganism cultures can be screened using a set of HPLC separations which are coupled parallel to CS-MAS as a fluorine specific detector and the ESI-MS as the molecular detection. This makes it possible to identify fluorine containing species without prior knowledge of the compounds and the use of clean up procedures. Here specifically we identify traces of a fluorometabolite (5′-fluoro-5′-deoxy-adenosine, 5′-FDA) in complex media of Streptomyces cattleya without preconcentration and target analysis.

AB - The lack of specific fluorine detection at trace levels hampers the screening of microorganisms which can form novel fluorometabolites. So far only target analysis by ESI-MS using accurate mass and certain fragmentation or preconcentration and clean-up procedures has been used to perform 19F and 13C/1H NMR for the identification of novel fluorocompounds. Here we demonstrate that the analysis of complex media from microorganism cultures can be screened using a set of HPLC separations which are coupled parallel to CS-MAS as a fluorine specific detector and the ESI-MS as the molecular detection. This makes it possible to identify fluorine containing species without prior knowledge of the compounds and the use of clean up procedures. Here specifically we identify traces of a fluorometabolite (5′-fluoro-5′-deoxy-adenosine, 5′-FDA) in complex media of Streptomyces cattleya without preconcentration and target analysis.

U2 - 10.1039/C3JA50086A

DO - 10.1039/C3JA50086A

M3 - Article

VL - 28

SP - 877

EP - 882

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 6

ER -