Modulation of glutathione peroxidase activity in human vascular endothelial cells by fatty acids and the cytokine interleukin-1 beta

A J Crosby, K Wahle, G G Duthie

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Abstract

Objectives of this study were (a) to assess the extent of oxidative stress elicited in human endothelial cells by n - 3, n - 6 and n - 9 mono- and polyunsaturated fatty acids and by interleukin-1 beta and (b) to determine how such stress influenced glutathione peroxidase (GSHPx) activity, Fatty acids were co-incubated with human vascular endothelial cells (HUVEC) for 24 h. Lipid peroxidation, monitored as conjugated diene (CD) formation, increased 3-4-fold with increasing eicosapentaenoic and docosahexaenoic acids; 2-3-fold with linoleic acid; decreased by 50% with arachidonic acid and was unchanged with oleic acid. Changes in glutathione peroxidase activity mirrored conjugated diene formation in the HUVEC incubated with fatty acids. Interleukin-lp also increased glutathione peroxidase and conjugated diene formation; the latter increased enzyme activity dose-dependently suggesting a possible role for this oxidation product in the induction of glutathione peroxidase activity. The ability of fish oil fatty acids to induce antioxidant enzymes, particularly those of the glutathione redox system, may be an important mechanism protecting cells and tissues against oxidative and inflammatory cytokine elicited damage.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume1303
Issue number3
Publication statusPublished - 18 Oct 1996

Keywords

  • unsaturated fatty acid
  • cytokine
  • endothelial cell
  • antioxidant
  • antioxidant enzyme
  • conjugated diene
  • (human)
  • low-density-lipoprotein
  • coronary heart-disease
  • superoxide-dismutase
  • antioxidant enzymes
  • lipid-peroxidation
  • redox cycle
  • culture
  • N-3

Cite this

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title = "Modulation of glutathione peroxidase activity in human vascular endothelial cells by fatty acids and the cytokine interleukin-1 beta",
abstract = "Objectives of this study were (a) to assess the extent of oxidative stress elicited in human endothelial cells by n - 3, n - 6 and n - 9 mono- and polyunsaturated fatty acids and by interleukin-1 beta and (b) to determine how such stress influenced glutathione peroxidase (GSHPx) activity, Fatty acids were co-incubated with human vascular endothelial cells (HUVEC) for 24 h. Lipid peroxidation, monitored as conjugated diene (CD) formation, increased 3-4-fold with increasing eicosapentaenoic and docosahexaenoic acids; 2-3-fold with linoleic acid; decreased by 50{\%} with arachidonic acid and was unchanged with oleic acid. Changes in glutathione peroxidase activity mirrored conjugated diene formation in the HUVEC incubated with fatty acids. Interleukin-lp also increased glutathione peroxidase and conjugated diene formation; the latter increased enzyme activity dose-dependently suggesting a possible role for this oxidation product in the induction of glutathione peroxidase activity. The ability of fish oil fatty acids to induce antioxidant enzymes, particularly those of the glutathione redox system, may be an important mechanism protecting cells and tissues against oxidative and inflammatory cytokine elicited damage.",
keywords = "unsaturated fatty acid, cytokine, endothelial cell, antioxidant, antioxidant enzyme, conjugated diene, (human), low-density-lipoprotein, coronary heart-disease, superoxide-dismutase, antioxidant enzymes, lipid-peroxidation, redox cycle, culture, N-3",
author = "Crosby, {A J} and K Wahle and Duthie, {G G}",
year = "1996",
month = "10",
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language = "English",
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pages = "187--192",
journal = "Biochimica et Biophysica Acta - Lipids and Lipid Metabolism",
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TY - JOUR

T1 - Modulation of glutathione peroxidase activity in human vascular endothelial cells by fatty acids and the cytokine interleukin-1 beta

AU - Crosby, A J

AU - Wahle, K

AU - Duthie, G G

PY - 1996/10/18

Y1 - 1996/10/18

N2 - Objectives of this study were (a) to assess the extent of oxidative stress elicited in human endothelial cells by n - 3, n - 6 and n - 9 mono- and polyunsaturated fatty acids and by interleukin-1 beta and (b) to determine how such stress influenced glutathione peroxidase (GSHPx) activity, Fatty acids were co-incubated with human vascular endothelial cells (HUVEC) for 24 h. Lipid peroxidation, monitored as conjugated diene (CD) formation, increased 3-4-fold with increasing eicosapentaenoic and docosahexaenoic acids; 2-3-fold with linoleic acid; decreased by 50% with arachidonic acid and was unchanged with oleic acid. Changes in glutathione peroxidase activity mirrored conjugated diene formation in the HUVEC incubated with fatty acids. Interleukin-lp also increased glutathione peroxidase and conjugated diene formation; the latter increased enzyme activity dose-dependently suggesting a possible role for this oxidation product in the induction of glutathione peroxidase activity. The ability of fish oil fatty acids to induce antioxidant enzymes, particularly those of the glutathione redox system, may be an important mechanism protecting cells and tissues against oxidative and inflammatory cytokine elicited damage.

AB - Objectives of this study were (a) to assess the extent of oxidative stress elicited in human endothelial cells by n - 3, n - 6 and n - 9 mono- and polyunsaturated fatty acids and by interleukin-1 beta and (b) to determine how such stress influenced glutathione peroxidase (GSHPx) activity, Fatty acids were co-incubated with human vascular endothelial cells (HUVEC) for 24 h. Lipid peroxidation, monitored as conjugated diene (CD) formation, increased 3-4-fold with increasing eicosapentaenoic and docosahexaenoic acids; 2-3-fold with linoleic acid; decreased by 50% with arachidonic acid and was unchanged with oleic acid. Changes in glutathione peroxidase activity mirrored conjugated diene formation in the HUVEC incubated with fatty acids. Interleukin-lp also increased glutathione peroxidase and conjugated diene formation; the latter increased enzyme activity dose-dependently suggesting a possible role for this oxidation product in the induction of glutathione peroxidase activity. The ability of fish oil fatty acids to induce antioxidant enzymes, particularly those of the glutathione redox system, may be an important mechanism protecting cells and tissues against oxidative and inflammatory cytokine elicited damage.

KW - unsaturated fatty acid

KW - cytokine

KW - endothelial cell

KW - antioxidant

KW - antioxidant enzyme

KW - conjugated diene

KW - (human)

KW - low-density-lipoprotein

KW - coronary heart-disease

KW - superoxide-dismutase

KW - antioxidant enzymes

KW - lipid-peroxidation

KW - redox cycle

KW - culture

KW - N-3

M3 - Article

VL - 1303

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EP - 192

JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

SN - 0005-2760

IS - 3

ER -