Molecular cloning of a myoD-like gene from the parasitic nematode, Trichinella spiralis

Bernadette Connolly, K Trenholme, D F Smith

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


The infective larval stage of the nematode Trichinella spiralis is an intracellular parasite of skeletal muscle cells. Infection with T. spiralis results in dedifferentiation of the host cell and the formation of a host/parasite complex. A gene encoding a T. spiralis Helix-Loop-helix (HLH) protein with homology to the myogenic transcription factor, MyoD, and to the Caenorhabditis elegans protein, CeMyoD, has been identified and partially characterized. The tsmyd-1 gene is expressed constitutively during the muscle-larval and adult stages. A purified recombinant Tsmyd-1 protein, expressed in Escherichia coli, binds to a high affinity mouse MyoD DNA binding site in vitro. The present study describes the first HLH gene to be identified in Trichinella.
Original languageEnglish
Pages (from-to)137-149
Number of pages13
JournalMolecular and Biochemical Parasitology
Issue number2
Publication statusPublished - 30 Oct 1996


  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA
  • DNA Primers
  • DNA, Complementary
  • Escherichia coli
  • Gene Expression Regulation, Developmental
  • Genes, Helminth
  • Helix-Loop-Helix Motifs
  • Helminth Proteins
  • Mice
  • Molecular Sequence Data
  • MyoD Protein
  • Protein Binding
  • Recombinant Proteins
  • Sequence Homology, Amino Acid
  • Trichinella spiralis
  • Trichinella
  • Muscle-stage larva
  • Basic helix-loop-helix
  • MyoD
  • CeMyoD
  • Nau


Dive into the research topics of 'Molecular cloning of a myoD-like gene from the parasitic nematode, Trichinella spiralis'. Together they form a unique fingerprint.

Cite this