Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis

J D Johnston, B B Tournier, H Andersson, M Masson-Pevet, G A Lincoln, D G Hazlerigg

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

In mammals, changing day length modulates endocrine rhythms via nocturnal melatonin secretion. Studies of the pituitary pars tuberalis (PT) suggest that melatonin-regulated clock gene expression is critical to this process. Here, we considered whether clock gene rhythms continue in the PT in the absence of melatonin and whether the effects of melatonin on the expression of these genes are temporally gated. Soay sheep acclimated to long photoperiod (LP) were transferred to constant light for 24 h, suppressing endogenous melatonin secretion. Animals were infused with melatonin at 4-h intervals across the final 24 h, and killed 3 h after infusion. The expression of five clock genes (Per1, Per2, Cry1, Rev-erb alpha, and Bmal1) was measured by in situ hybridization. In sham-treated animals, PT expression of Per1, Per2, and Rev-erb alpha showed pronounced temporal variation despite the absence of melatonin, with peak times occurring earlier than predicted under LP. The time of peak Bmal1 expression remained LP-like, whereas Cry1 expression was continually low. Melatonin infusion induced Cry1 expression at all times and suppressed other genes, but only when they showed high expression in sham-treated animals. Hence, 3 h after melatonin treatment, clock gene profiles were driven to a similar state, irrespective of infusion time. In contrast to the PT, melatonin infusions had no clear effect on clock gene expression in the suprachiasmatic nuclei. Our results provide the first example of acute sensitivity of multiple clock genes to one endocrine stimulus and suggest that rising melatonin levels may reset circadian rhythms in the PT, independently of previous phase.

Original languageEnglish
Pages (from-to)959-965
Number of pages7
JournalEndocrinology
Volume147
DOIs
Publication statusPublished - 2006

Keywords

  • DOPAMINERGIC NEURON ACTIVITY
  • DECODING PHOTOPERIODIC TIME
  • PROLACTIN SECRETION
  • PITUITARY-GLAND
  • SUPRACHIASMATIC NUCLEUS
  • INDIVIDUAL FIBROBLASTS
  • CIRCADIAN-RHYTHMS
  • SEASONAL TIMER
  • SYRIAN-HAMSTER
  • CELLS

Cite this

Johnston, J. D., Tournier, B. B., Andersson, H., Masson-Pevet, M., Lincoln, G. A., & Hazlerigg, D. G. (2006). Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis. Endocrinology, 147, 959-965. https://doi.org/10.1210/en.2005-1100

Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis. / Johnston, J D ; Tournier, B B ; Andersson, H ; Masson-Pevet, M ; Lincoln, G A ; Hazlerigg, D G .

In: Endocrinology, Vol. 147, 2006, p. 959-965.

Research output: Contribution to journalArticle

Johnston, JD, Tournier, BB, Andersson, H, Masson-Pevet, M, Lincoln, GA & Hazlerigg, DG 2006, 'Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis', Endocrinology, vol. 147, pp. 959-965. https://doi.org/10.1210/en.2005-1100
Johnston JD, Tournier BB, Andersson H, Masson-Pevet M, Lincoln GA, Hazlerigg DG. Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis. Endocrinology. 2006;147:959-965. https://doi.org/10.1210/en.2005-1100
Johnston, J D ; Tournier, B B ; Andersson, H ; Masson-Pevet, M ; Lincoln, G A ; Hazlerigg, D G . / Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis. In: Endocrinology. 2006 ; Vol. 147. pp. 959-965.
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AU - Johnston, J D

AU - Tournier, B B

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AU - Lincoln, G A

AU - Hazlerigg, D G

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AB - In mammals, changing day length modulates endocrine rhythms via nocturnal melatonin secretion. Studies of the pituitary pars tuberalis (PT) suggest that melatonin-regulated clock gene expression is critical to this process. Here, we considered whether clock gene rhythms continue in the PT in the absence of melatonin and whether the effects of melatonin on the expression of these genes are temporally gated. Soay sheep acclimated to long photoperiod (LP) were transferred to constant light for 24 h, suppressing endogenous melatonin secretion. Animals were infused with melatonin at 4-h intervals across the final 24 h, and killed 3 h after infusion. The expression of five clock genes (Per1, Per2, Cry1, Rev-erb alpha, and Bmal1) was measured by in situ hybridization. In sham-treated animals, PT expression of Per1, Per2, and Rev-erb alpha showed pronounced temporal variation despite the absence of melatonin, with peak times occurring earlier than predicted under LP. The time of peak Bmal1 expression remained LP-like, whereas Cry1 expression was continually low. Melatonin infusion induced Cry1 expression at all times and suppressed other genes, but only when they showed high expression in sham-treated animals. Hence, 3 h after melatonin treatment, clock gene profiles were driven to a similar state, irrespective of infusion time. In contrast to the PT, melatonin infusions had no clear effect on clock gene expression in the suprachiasmatic nuclei. Our results provide the first example of acute sensitivity of multiple clock genes to one endocrine stimulus and suggest that rising melatonin levels may reset circadian rhythms in the PT, independently of previous phase.

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KW - PROLACTIN SECRETION

KW - PITUITARY-GLAND

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KW - INDIVIDUAL FIBROBLASTS

KW - CIRCADIAN-RHYTHMS

KW - SEASONAL TIMER

KW - SYRIAN-HAMSTER

KW - CELLS

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