MULTIPLICITY AND EXPRESSION OF XYLANASES IN THE RUMEN CELLULOLYTIC BACTERIUM RUMINOCOCCUS-FLAVEFACIENS

H J FLINT, J X ZHANG, J MARTIN

Research output: Contribution to journalArticle

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Abstract

The cellulolytic rumen bacterium Ruminococcus flavefaciens 17 was found to produce multiple xylanases ranging in apparent molecular weight from 55 to 200 kDa. A 55 kDa xylanase showed constitutive synthesis, but formation of the larger enzymes was increased in cultures grown with avicel, straw, or xylan, compared with cellobiose, as the energy source. At least six xylanases were detected in cultures grown with oat straw or oat xylan. Polyclonal antibodies were raised against the amino (A) or carboxy terminal (C) domains of the bifunctional XYNA product of the cloned R. flavefaciens xynA gene. Both antibody preparations recognized several xylanases larger than 80 kDa from R. flavefaciens cells grown with avicel, straw, or xylan, indicating the production of multiple, antigenically related enzymes during growth on these substrates. Neither antibody preparation recognized the constitutive 55-kDa xylanase.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalCurrent Microbiology
Volume29
Issue number3
Publication statusPublished - Sep 1994

Keywords

  • CELL-WALLS
  • DOMAINS
  • SEQUENCE
  • ENZYME
  • ACID
  • GENE

Cite this

MULTIPLICITY AND EXPRESSION OF XYLANASES IN THE RUMEN CELLULOLYTIC BACTERIUM RUMINOCOCCUS-FLAVEFACIENS. / FLINT, H J ; ZHANG, J X ; MARTIN, J .

In: Current Microbiology, Vol. 29, No. 3, 09.1994, p. 139-143.

Research output: Contribution to journalArticle

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abstract = "The cellulolytic rumen bacterium Ruminococcus flavefaciens 17 was found to produce multiple xylanases ranging in apparent molecular weight from 55 to 200 kDa. A 55 kDa xylanase showed constitutive synthesis, but formation of the larger enzymes was increased in cultures grown with avicel, straw, or xylan, compared with cellobiose, as the energy source. At least six xylanases were detected in cultures grown with oat straw or oat xylan. Polyclonal antibodies were raised against the amino (A) or carboxy terminal (C) domains of the bifunctional XYNA product of the cloned R. flavefaciens xynA gene. Both antibody preparations recognized several xylanases larger than 80 kDa from R. flavefaciens cells grown with avicel, straw, or xylan, indicating the production of multiple, antigenically related enzymes during growth on these substrates. Neither antibody preparation recognized the constitutive 55-kDa xylanase.",
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AB - The cellulolytic rumen bacterium Ruminococcus flavefaciens 17 was found to produce multiple xylanases ranging in apparent molecular weight from 55 to 200 kDa. A 55 kDa xylanase showed constitutive synthesis, but formation of the larger enzymes was increased in cultures grown with avicel, straw, or xylan, compared with cellobiose, as the energy source. At least six xylanases were detected in cultures grown with oat straw or oat xylan. Polyclonal antibodies were raised against the amino (A) or carboxy terminal (C) domains of the bifunctional XYNA product of the cloned R. flavefaciens xynA gene. Both antibody preparations recognized several xylanases larger than 80 kDa from R. flavefaciens cells grown with avicel, straw, or xylan, indicating the production of multiple, antigenically related enzymes during growth on these substrates. Neither antibody preparation recognized the constitutive 55-kDa xylanase.

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