This study shows the importance of resolving catalytic performance in the regeneration of the reduced form of nicotinamide adenine dinucleotide (NADH) through activity measurements based on NAD+ conversion and the closure of mass balance via by-product quantification. This approach is applied to assess the performance of supported platinum catalysts with varying points of zero charge, utilizing H2 as a reductant. It was found that Pt/SiO2, which exhibits a net negative charge under the reaction conditions, outperforms the neutral Pt/C and positively charged Pt/MgO due to the favorable electrostatic attraction between the catalyst surface and positively charged (+1) nicotinamide ring. NMR spectroscopy identifies side-products formed during NAD+ hydrogenation.
- supported Pt
- mass balance